Metabolomic association analysis reveals defense related secondary metabolic reprogramming in callus of Scutellaria baicalensis Georgi induced by endophytic Pseudomonas sp. 2B

Xu, Jingyuan; Shi, Ruoyun; Cheng, Yijie; GuangHui, Xie; Zhu, Yan; Qin, Min-Jian

doi:10.1016/j.indcrop.2022.115409

Cited by 4 publications

(5 citation statements)

References 62 publications

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“…In addition, some easily miscible products, such as the plants in the genus of Dendrobium ( Zuo, Zu, Liu, Song, & Yuan, 2021 ) and Ferula ( Wang, Zhang, He, & Sheng, 2018 , Wang et al, 2018 ), can also be considered to establish relevant microbial standards for identification. Finally, the rhizosphere microorganisms were obtained by plate scribing method and verified by callus culture and cultivation experiments ( Guo, Liu, Tang, Chen, & Pei, 2017 , Xu et al, 2022 , Xu et al, 2022 ).…”

Section: Determination and Research Methods Of Micro-markersmentioning

confidence: 99%

“…Exploring the common rhizosphere microorganisms of medicinal plants is of great value for solving the cultivation problems of endangered plants. The imbalance in the regulation of canopy density and soil pH-bacterial diversity might exacerbate the endangered status of Scutellaria tsinyunensis C. Y. Wu & S. Chow ( Xu et al, 2022 , Xu et al, 2022 ). Furthermore, it was revealed that Haliangium and Candidatus Koribacter are important genera for the protection of S. tsinyunensis , which are of great significance for promoting the growth of S. tsinyunensis and protecting the endangered plants.…”

Section: Role and Significance Of Micro-markersmentioning

confidence: 99%

See 1 more Smart Citation

Rhizosphere microbial markers (micro-markers): A new physical examination indicator for traditional Chinese medicines

Yang,

Zheng,

Sun

et al. 2024

Chinese Herbal Medicines

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Section: Determination and Research Methods Of Micro-markersmentioning

confidence: 99%

Section: Role and Significance Of Micro-markersmentioning

confidence: 99%

Rhizosphere microbial markers (micro-markers): A new physical examination indicator for traditional Chinese medicines

Yang,

Zheng,

Sun

et al. 2024

Chinese Herbal Medicines

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“…Calli induction in S. baicalensis was performed as previously described, with some modifications ( Wan et al., 2012 ). Briefly, stem explants (grown in vitro ) were cut aseptically into approximately 10 mm segments and placed on MS medium supplemented with 2.0 mg/L 6-BA (6-Benzylaminopurine) (Xiya Reagent, Linyi, China) and 1.0 mg/L NAA (-naphthalene acetic acid) (Solarbio, Beijing, China), which could promote the induction and proliferation of calli ( Xu et al., 2022 ). After 2 weeks, S. baicalensis calli were subcultured in the same culture medium at 25 ± 1°C.…”

Section: Methodsmentioning

confidence: 99%

Effects of elicitors from culture filtrate of Fusarium solani CL105 on flavonoid production of Scutellaria baicalensis calli

Cui,

Zhang,

Sun

et al. 2024

Front. Plant Sci.

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IntroductionEndophytic fungi can promote secondary metabolite accumulation in medicinal plants. Previously, we observed that the culture filtrate of Fusarium solani CL105 promoted flavonoid production in Scutellaria baicalensis calli. However, the active ingredients and mechanisms associated with this secondary metabolite accumulation remain unclear.MethodsThis study evaluates the effects of different elicitors from the culture filtrate of F. solani CL105 namely, exopolysaccharide (EPS), exoprotein (EP), and other parts (OP), on the flavonoid production in S. baicalensis calli by HPLC. Subsequently, the underlying mechanism of EPS induced flavonoid production in S. baicalensis calli was revealed by transcriptomics and RT-PCR.Results and discussionThe results indicated a significant increase in flavonoid production in S. baicalensis calli following treatment with EPS. Baicalin (1.40 fold), wogonoside (1.91 fold), and wogonin (2.76 fold) were most significantly up-regulated compared with the control. Transcriptome analysis further revealed up-regulation of key enzyme genes (CHS, CHI, FNS, and F6H) involved in flavonoid synthesis after 5 days of EPS treatment. Moreover, the expression of GA2ox and CYP707A—genes involved in gibberellin acid (GA) and abscisic acid biosynthesis (ABA), respectively—were significantly up-regulated. The expression levels of certain transcription factors, including MYB3, MYB8, and MYB13, were also significantly higher than in controls. Our results indicated that EPS was a main active elicitor involved in promoting flavonoid production in S. baicalensis calli. We postulated that EPS might stimulate the expression of MYB3, MYB8, MYB13, GA2ox, and CYP707A, leading to markedly upregulated CHS, CHI, FNS, and F6H expression levels, ultimately promoting flavonoid synthesis. This study provides a novel avenue for large-scale in vitro production of flavonoids in S. baicalensis.

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“…Moreover, secondary metabolites derived from endophytic bacterial cultures have demonstrated cytotoxic, antimicrobial, antiviral, and anticancer activities in previous studies [4]. The growing interest in endophytic bacteria emphasizes their utilization due to their ability to enhance plant defense responses through the formation of secondary metabolites [5]. Endophytic bacteria can produce new secondary metabolites with significant bioactivity potential [6].…”

Section: Introductionmentioning

confidence: 99%

“…Secondary metabolites play a crucial role in plants overcoming diverse environmental stresses. The metabolite-based approach can be used to characterize metabolic alterations, encompassing compounds such as alkaloids, phenolics, terpenoid glycosides, and flavonoids [5]. According to Rahayu et al [8], Hibiscus leaves contain alkaloids, flavonoids, saponins, and tannins.…”

Section: Introductionmentioning

confidence: 99%

Endophytic Bacteria Producing Alkaloid as Antibacterial and Antioxidant Agent from Hibiscus tiliaceus Leaves

Sarjono,

Anggraeni,

Ismiyarto

et al. 2024

J. Kim. Sains Apl.

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Hibiscus tiliaceus is known to have antibacterial activity. One of the antibacterial compounds contained in the hibiscus plant is alkaloid. Alkaloids can also be obtained from endophytic bacteria that are in symbiosis with hibiscus leaves. It is known that endophytes can produce the same or similar secondary metabolites as their host plants. Medicinal plants have been proven to be a source of microorganisms that can make certain metabolites. Our previous research succeeded in isolating the endophytic bacteria Staphylococcus warneri strain 25 from waru leaves (Hibiscus tiliaceus), which had antioxidant and antibacterial activity and contained saponins and alkaloids. In this study, alkaloids were extracted from the endophytic bacteria found in waru leaves. Subsequently, their bioactivity was assessed for antibacterial properties against Escherichia coli, Salmonella typhi, and Staphylococcus aureus using the well diffusion method. Additionally, their antioxidant activity was evaluated through the DPPH method, with gallic acid serving as the standard. A cyclic amine alkaloid compound was isolated, exhibiting a Minimum Inhibitory Concentration (MIC) of 23 ppm against E. coli, S. typhi, and S. aureus bacteria, accompanied by inhibitory zones measuring 7 mm, 8 mm, and 6 mm, respectively. Additionally, the compound had very strong antioxidant activity, with an IC50 value of 50.11 ppm.

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Metabolomic association analysis reveals defense related secondary metabolic reprogramming in callus of Scutellaria baicalensis Georgi induced by endophytic Pseudomonas sp. 2B

Cited by 4 publications

References 62 publications

Rhizosphere microbial markers (micro-markers): A new physical examination indicator for traditional Chinese medicines

Rhizosphere microbial markers (micro-markers): A new physical examination indicator for traditional Chinese medicines

Effects of elicitors from culture filtrate of Fusarium solani CL105 on flavonoid production of Scutellaria baicalensis calli

Endophytic Bacteria Producing Alkaloid as Antibacterial and Antioxidant Agent from Hibiscus tiliaceus Leaves

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