Chlorella is an attractive organism for complex recombinant protein production because of its eukaryotic characteristics and low cost for large-scale culture. Protoplasts of C. ellipsoidea were transformed with a vector containing the flounder growth hormone gene (fGH) under the control of the cauliflower mosaic virus 35S promoter, and the phleomycin resistance Sh ble gene under the control of the Chlamydomonas RBCS2 gene promoter. The presence of introduced DNA was first determined by PCR amplification of both the fGH and Sh ble genes from genomic DNA isolated from transformants and fGH protein expression was detected by immunoblot analysis. Over 400 microg of fGH protein expression per one liter culture containing 1 x 10(8) cells/ml was estimated by ELISA. Stable integration of introduced DNA was confirmed by Southern blot analysis of genomic DNA digested with restriction enzymes. The introduced DNA and fGH expression were detected after seven successive transfers in media devoid of phleomycin, but stably remained in the presence of the antibiotic. Flounder fry fed on the transformed Chlorella revealed a 25% growth increase after 30 days of feeding.
Artificial synapses
based on ferroelectric Schottky barrier field-effect
transistors (FE-SBFETs) are experimentally demonstrated. The FE-SBFETs
employ single-crystalline NiSi2 contacts with an atomically
flat interface to Si and Hf0.5Zr0.5O2 ferroelectric layers on silicon-on-insulator substrates. The ferroelectric
polarization switching dynamics gradually modulate the NiSi2/Si Schottky barriers and the potential of the channel, thus programming
the device conductance with input voltage pulses. The short-term synaptic
plasticity is characterized in terms of excitatory/inhibitory post-synaptic
current (EPSC) and paired-pulse facilitation/depression. The EPSC
amplitude shows a linear response to the amplitude of the pre-synaptic
spike. Very low energy/spike consumption as small as ∼2 fJ
is achieved, demonstrating high energy efficiency. Long-term potentiation/depression
results show very high endurance and very small cycle-to-cycle variations
(∼1%) after 105 pulse measurements. Furthermore,
spike-timing-dependent plasticity is also emulated using the gate
voltage pulse as the pre-synaptic spike and the drain voltage pulse
as the post-synaptic spikes. These findings indicate that FE-SBFET
synapses have high potential for future neuromorphic computing applications.
Harvesting the full potential of single crystal semiconductor nanowires (NWs) for advanced nanoscale field-effect transistors (FETs) requires a smart combination of charge control architecture and functional semiconductors. In this article, high performance vertical gate-all-2 around nanowire p-type pFETs are presented. The device concept is based on advanced Ge0.92Sn0.08/Ge group IV epitaxial heterostructures, employing quasi-one-dimensional semiconductor nanowires fabricated with a top-down approach. The advantage of using a heterostructure is the possibility of electronic band engineering with band offsets tunable by changing the semiconductor stoichiometry and elastic strain. The use of a Ge0.92Sn0.08 layer as the source in GeSn/Ge NW pFETs results in a small subthreshold slope of 72 mV/dec and a high ION/IOFF ratio of 3×10 6 . A ~32% drive current enhancement is obtained compared to vertical Ge homojunction NW control devices. More interestingly, the drain-induced-barrier lowering is much smaller with GeSn instead of Ge as the source. The general improvement of the transistor's key figures of merits originates from the valence band offset at the Ge0.92Sn0.08/Ge heterojunction, as well as from a smaller NiGeSn/GeSn contact resistivity.
Vibrio anguillarum, an opportunistic fish pathogen, is the main species responsible for vibriosis, a disease that affects feral and farmed fish and shellfish, and causes considerable economic losses in marine aquaculture. In this study, we used polymerase chain reaction (PCR) to detect V. anguillarum. PCR specificity was evaluated by amplifying the rpoS gene, a general stress regulator, in six strains of V. anguillarum and 36 other bacterial species. PCR amplified a species-specific fragment (689 bp) from V. anguillarum. Furthermore, the PCR assay was sensitive enough to detect rpoS expression from 3 pg of genomic DNA, or from six colony-forming units (CFU) mL(-1) of cultured V. anguillarum. However, the assay was less sensitive when genomic DNA from the infected flounder and prawn was used (limit of detection, 50 ng and 10 ng g(-1) tissue, respectively). These data demonstrate that PCR amplification of the rpoS gene is a sensitive and species-specific method to detect V. anguillarum in practical situations.
The continued downscaling of silicon CMOS technology presents challenges for achieving the required low power consumption. While high mobility channel materials hold promise for improved device performance at low power levels, a material system which enables both high mobility n-FETs and p-FETs, that is compatible with Si technology and can be readily integrated into existing fabrication lines is required. Here, we present high performance, vertical nanowire gate-all-around FETs based on the GeSn-material system grown on Si. While the p-FET transconductance is increased to 850 µS/µm by exploiting the small band gap of GeSn as source yielding high injection velocities, the mobility in n-FETs is increased 2.5-fold compared to a Ge reference device, by using GeSn as channel material. The potential of the material system for a future beyond Si CMOS logic and quantum computing applications is demonstrated via a GeSn inverter and steep switching at cryogenic temperatures, respectively.
Fully silicided source/drain Si gate-all-around (GAA) nanowire (NW) p-FETs with NW diameter of 5 nm are fabricated and characterized from room temperature (RT) down to 5.5 K. Thanks to the improved electrostatics by the scaled NW and 3D GAA structure, close to ideal transfer characteristics are obtained at both RT and 5.5 K with a sharp switching. Benefiting from less defects in Si created by the implantation into silicide (IIS) process, the band tail effects and neutral defects scattering are suppressed. Therefore, the fabricated Si GAA NW p-FETs provide very low subthreshold swing SS of 3.4 mV/dec in the weak inversion region and an average SS th of 14 mV/dec measured from the off-state to the threshold voltage, as well as an improved transconductance G m at 5.5 K.
Neuromorphic computing employs a great number of artificial synapses which transfer information between neurons. Conventional two‐ or three‐terminal artificial synapses with homosynaptic plasticity suffer from a positive feedback loop problem. Synapses with heterosynaptic plasticity are thus required to perform learning, processing and modulating simultaneously. Here, complementary metal‐oxide‐semiconductor compatible artificial synapses based on ferroelectric polarization modulated Schottky diodes (FEMOD) on silicon, which enables heterosynaptic plasticity with multi‐functionalities, high endurance, low power consumption, and high speed, are presented. High accuracy is obtained in the supervised learning simulation of artificial neural networks due to the large number of conductance states, good linearity, and small variations of FEMOD synapses. Boolean functions are demonstrated with only one or two FEMOD devices operating at low voltage and low power consumption. The proposed device structure performs multi‐functions of biological synapse and Boolean logic, thus provides high potential for the future large scale and low power neuromorphic computing applications.
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