Vogesella fluminis sp. nov., isolated from a freshwater river, and emended description of the genus Vogesella The genus Vogesella, first described by Grimes et al. , 1997;Chou et al., 2008Chou et al., , 2009Jørgensen et al., 2010;Subhash et al., 2013). The present study was carried out to clarify the taxonomic position of the Vogesella-like bacterial strain Npb-07 T by a polyphasic taxonomic approach.During our investigations on the biodiversity of bacteria in a freshwater river, the Beishi River (GPS location: 22 u 229 130 N 120 u 359 310 E) in the vicinity of Pingtung, Taiwan, several white-coloured bacterial colonies were isolated on R2A agar (BD Difco). Strains showing similar colony morphology were segregated and a representative strain, designated Npb-07 T , was selected for detailed taxonomic analyses. Strain Npb-07 T was maintained on R2A agar at 25 u C. The strain was preserved at 280 u C as a 20 % (v/v) glycerol suspension in R2A broth or by lyophilization with 20 % (w/v) skimmed milk. V. indigofera ATCC 19706T was obtained from the American type Culture

A bacterial strain designated CMJ-9T was isolated from a freshwater shrimp culture pond inTaiwan and characterized using a polyphasic taxonomic approach. Cells of strain CMJ-9 T were strictly aerobic, Gram-negative, motile by a single polar flagellum, poly-b-hydroxybutyratecontaining and formed light-yellow colonies. Growth occurred at 10-37 6C (optimum, 20-30 6C), with 0-0.8 % NaCl (optimum, 0-0.1 %) and at pH 6.0-9.0 (optimum, pH 6.0-7.0). Phylogenetic analyses based on 16S rRNA gene sequences showed that strain CMJ-9 T belonged to the genus Undibacterium, and its closest neighbour was Undibacterium seohonense SHS5-24 T , with 96.7 % sequence similarity. The predominant cellular fatty acids were summed feature 3 (C 16 : 1 v7c and/or C 16 : 1 v6c) and C 16 : 0 . The major cellular hydroxy fatty acid was C 10 : 0 3-OH. The polar lipid profile consisted of the predominant lipids phosphatidylethanolamine, phosphatidylglycerol and diphosphatidylglycerol. The polyamine profile was composed of the major compound putrescine and moderate amounts of 2-hydroxyputrescine. The major respiratory quinone was Q-8 and the DNA G+C content was 47.7 mol%. On the basis of the phylogenetic and phenotypic data, strain CMJ-9 T should be classified within a novel species, for which the name Undibacterium macrobrachii sp. nov. is proposed. The type strain is CMJ-9 T (5BCRC 80406 T 5LMG 26891 T 5KCTC 23916 T ).

A bacterial strain, designated Npb-03T, was isolated from a freshwater river in Taiwan and was characterized using a polyphasic taxonomic approach. The cells were Gram-reaction-negative, straight rod-shaped, non-motile, non-spore-forming and facultatively anaerobic. Growth occurred at 10–37 °C (optimum, 30–35 °C), at pH 6.0–8.0 (optimum, pH 6.0–7.0) and with 0–1.0 % NaCl (optimum, 0 %). The predominant fatty acids were summed feature 3 (comprising C16 : 1ω7c and/or C16 : 1ω6c) and C16 : 0. The major isoprenoid quinone was Q-8 and the DNA G+C content was 64.1 mol%. The polar lipid profile consisted of a mixture of phosphatidylethanolamine, phosphatidylglycerol, diphosphatidylglycerol, an uncharacterized aminolipid and three uncharacterized phospholipids. The major polyamines were putrescine, 2-hydroxyputrescine, cadaverine and spermidine. Phylogenetic analyses based on 16S rRNA gene sequences showed that strain Npb-03T forms a distinct lineage with respect to closely related genera within the family Neisseriaceae of the class Betaproteobacteria , most closely related to the genera Aquaspirillum , Laribacter , Leeia and Microvirgula , and the levels of 16S rRNA gene sequence similarity with respect to the type species of related genera are less than 93 %. On the basis of the genotypic and phenotypic data, strain Npb-03T represents a novel genus and species of the family Neisseriaceae , for which the name Rivicola pingtungensis gen. nov., sp. nov. is proposed. The type strain is Npb-03T ( = BCRC 80376T = LMG 26668T = KCTC 23712T).

A bacterial strain designated T was isolated from a freshwater shrimp culture pond inTaiwan and characterized using a polyphasic taxonomic approach. Cells of strain CMJ-15 T were facultatively anaerobic, Gram-stain-negative and motile by a single polar flagellum and formed light-yellow colonies. Growth occurred at 20-40 6C (optimum, 25-30 6C), with 0-0.7 % NaCl (optimum, 0-0.2 %) and at pH 5.0-9.0 (optimum, pH 6.0-7.0). Phylogenetic analyses based on 16S rRNA gene sequences showed that strain CMJ-15 T belonged to the genus Undibacterium and its closest neighbour was Undibacterium oligocarboniphilum EM 1 T , with sequence similarity of 96.3 %. The predominant cellular fatty acids were summed feature 3 (C 16 : 1 v7c and/or C 16 : 1 v6c), C 16 : 0 and C 18 : 1 v7c. The major cellular hydroxy fatty acid was C 10 : 0 3-OH. The polar lipid profile consisted of phosphatidylethanolamine, phosphatidylglycerol and diphosphatidylglycerol. The polyamine profile was composed of the major compound putrescine and moderate amounts of 2-hydroxyputrescine. The major respiratory quinone was Q-8 and the DNA G+C content was 52.2 mol%. On the basis of these phylogenetic and phenotypic data, strain CMJ-15 T should be classified as representing a novel species, for which the name Undibacterium squillarum sp. nov. is proposed. The type strain is CMJ-15 T (5BCRC 80404 T 5LMG 26892T 5KCTC 23917 T ).

A bacterial strain, designated T , was isolated from a freshwater river in Taiwan and characterized in a taxonomic study using a polyphasic approach. Cells of strain Npb-02 T wereGram-stain-negative, aerobic, poly-b-hydroxybutyrate-accumulating, rod-shaped and non-motile. Growth occurred at 15-40 8C (optimum 25-30 8C), at pH 7.0-8.0 (optimum pH 7.0) and with 0-1 % NaCl (optimum 0.5 %). Phylogenetic analyses based on 16S rRNA gene sequences showed that strain Npb-02 T belonged to the genus Vogesella and was most closely related to The genus Vogesella (type species, Vogesella indigofera), first established by Grimes et al. (1997) , 1997; Chou et al., 2008Chou et al., , 2009Jørgensen et al., 2010;Sheu et al., 2013;Subhash et al., 2013). The present study was carried out to clarify the taxonomic position of a novel species of the genus Vogesella by using a polyphasic approach.During investigations on the biodiversity of bacteria in the Beishi River (GPS location: 228 229 130 N 1208 359 310 E; pH 7, 25 8C), a freshwater river in the vicinity of Pingtung, Taiwan, a novel bacterium designated Npb-02 T , was isolated and selected for detailed taxonomic analyses. This strain was isolated on R2A agar (BD Difco) after incubation at 25 8C for 3 days, sub-cultured under the same conditions and stored at 280 8C in R2A broth (BD Difco) with 20 % (v/v) glycerol, or stored following lyophilization.Genomic DNA was isolated using a bacterial genomic kit (DP02-150; GeneMark Technology) and the 16S rRNA gene sequence was obtained as described by Chen et al. (2001). Primers fD1 (59-AGAGTTTGATCCTGGCTCAG-39)Abbreviations: DPG, diphosphatidylglycerol; PE, phosphatidylethanolamine; PG, phosphatidylglycerol.The GenBank/EMBL/DDBJ accession number for the 16S rRNA gene sequence of strain T is LK056648.A supplementary figure and a supplementary table are available with the online Supplementary Material.