Rivicola pingtungensis gen. nov., sp. nov., a new member of the family Neisseriaceae isolated from a freshwater river

Abstract

A bacterial strain, designated Npb-03T, was isolated from a freshwater river in Taiwan and was characterized using a polyphasic taxonomic approach. The cells were Gram-reaction-negative, straight rod-shaped, non-motile, non-spore-forming and facultatively anaerobic. Growth occurred at 10–37 °C (optimum, 30–35 °C), at pH 6.0–8.0 (optimum, pH 6.0–7.0) and with 0–1.0 % NaCl (optimum, 0 %). The predominant fatty acids were summed feature 3 (comprising C16 : 1ω7c and/or C16 : 1ω6c) and C16 : 0. The major isoprenoid quinone was Q-8 and the DNA G+C content was 64.1 mol%. The polar lipid profile consisted of a mixture of phosphatidylethanolamine, phosphatidylglycerol, diphosphatidylglycerol, an uncharacterized aminolipid and three uncharacterized phospholipids. The major polyamines were putrescine, 2-hydroxyputrescine, cadaverine and spermidine. Phylogenetic analyses based on 16S rRNA gene sequences showed that strain Npb-03T forms a distinct lineage with respect to closely related genera within the family Neisseriaceae of the class Betaproteobacteria , most closely related to the genera Aquaspirillum , Laribacter , Leeia and Microvirgula , and the levels of 16S rRNA gene sequence similarity with respect to the type species of related genera are less than 93 %. On the basis of the genotypic and phenotypic data, strain Npb-03T represents a novel genus and species of the family Neisseriaceae , for which the name Rivicola pingtungensis gen. nov., sp. nov. is proposed. The type strain is Npb-03T ( = BCRC 80376T = LMG 26668T = KCTC 23712T).

Aquella oligotrophica gen. nov. sp. nov.: A new member of the family Neisseriaceae isolated from laboratory tap water

“…Several beta-lactamase gene were identified in the genome of P08 (Dong et al, 2015); 5, Rivicola (Sheu et al, 2014). +, Positive reaction; -, negative reaction; Q, quinone; ND, not determined.…”

“…Subsequently, 4 additional genera have been validly described and classified into family Neisseriaceae, namely Amantichitinum (Moß et al, ), Snodgrassella (Kwong & Moran, ), Rivicola (Sheu, Chen, Young, & Chen, ), Crenobacter (Dong et al, ), and Populibacter (Li, Xue, Sang, Lin, & Wang, ). At the time of writing, there are 17 validly described genera included in family Neisseriaceae.…”

“…Other than 16S rRNA gene sequence similarities and phylogenetic analysis, the major distinguishing features among genera for Neisseriaceae are cell morphology, biochemical characteristics, such as oxidase and catalase tests, glucose fermentation, nitrite reduction, and mol% G + C content of the genomic DNA (Garrity et al, ). Most strains inhabit indigenously in mucosal membranes of humans and animals, although environmental species were recently included in this family with representatives isolated from anthill, hot spring sediment, and freshwater river (Dong et al, ; Garrity et al, ; Moß et al, ; Sheu et al, ). During an assessment on laboratory tap water quality, a bacterial strain designated as P08 T was isolated on R2A agar.…”

“…Subsequently, 4 additional genera have been validly described and classified into family Neisseriaceae, namely Amantichitinum (Moß et al, 2013), Snodgrassella (Kwong & Moran, 2013), Rivicola (Sheu, Chen, Young, & Chen, 2014), Crenobacter (Dong et al, 2015), and…”

Craterilacuibacter sinensis gen. nov. sp. nov., isolated from a crater lake in China

“…The genus Neisseria was proposed as the type genus of this family by Trevisan in 1885 [2]. In the last 10 years, seven genera have been assigned to the family Neisseriaceae , namely Amantichitinum [3], Chitiniphilus [4], Chitinivorax [5], Crenobacter [6], Jeongeupia [7], Rivicola [8] and Snodgrassella [9]. The habitats of members of the family Neisseriaceae are variable, including soil, waste or lake water, and human and animal clinical specimens.…”