Craterilacuibacter sinensis gen. nov. sp. nov., isolated from a crater lake in China

Abstract

Two bacterial strains, designated B2N2-7T and B2N2-12, were isolated from Buteha crater lake in the Greater Khingan Mountain of China. The two strains were Gram-stain-negative, non-spore-forming, motile with a single polar flagellum, short rod-shaped bacteria. They were catalase- and oxidase-positive. Optimal growth occurred at 20–25 ℃, at pH 7.5–8.0 and with 0–1.0 % (w/v) NaCl. Based on phylogenomic analysis, strains B2N2-7T and B2N2-12 were assigned to the family Neisseriaceae , and their 16S rRNA gene sequences showed the highest similarities to that of Aquitalea denitrificans 5YN1-3T (<94.2 %). The predominant cellular fatty acids were C16 : 0 and summed feature 3 (comprising C16 : 1ω7c/C16 : 1 ω6c). The major respiratory quinone was ubiquinone 8 (Q-8). The polar lipids were phosphatidylglycerol (PG), diphosphatidylglycerol (DPG), phosphatidylethanolamine (PE), two unidentified aminophospholipids (APL) and some unidentified lipids (L). The genomic DNA G+C content of strain B2N2-7T was 59.4 mol% according to the genomic sequencing result. Based on the phylogenetic, genotypic and chemotaxonomic analyses, the two strains are proposed to represent a novel species of a new genus in the family Neisseriaceae , named Craterilacuibacter sinensis gen. nov., sp. nov. The type strain of Craterilacuibacter sinensis is B2N2-7T (=CGMCC 1.17189T=KCTC 73735T); B2N2-12 (=CGMCC 1.17190=KCTC 72734) is a second strain of the species.

Paralysiella testudinis gen. nov., sp. nov., isolated from the cloaca of a toad-headed turtle (Mesoclemmys nasuta)

“…Only limited information regarding polar lipid profiles is available about members of the Neisseriaceae. However, the polar lipid profile of strain 26B T differs not significantly from those of distantly related species of the family such as A. oligotropha [2], A. ursilacus [3], C. sinensis [4] and 'Populibacter cortices' [43]. Hence, it is supposed that the closely related species of strain 26B T have similar polar lipid profiles.…”

“…The family Neisseriaceae belongs to the Betaproteobacteria and so far 18 genera are assigned to this family (https://lpsn.dsmz.de/family/neisseriaceae) including Alysiella [1], Amantichitinum [2], Aquella [3], Bergeriella , Conchiformibius [1], Craterilacuibacter [4], Crenobacter [5], Eikenella [6], Kingella [7], Morococcus [8], Neisseria [9], Prolinoborus [10], Rivicola [11] , Simonsiella [12], Snodgrassella [13], Stenoxybacter [14], Uruburuella [15] and Vitreoscilla [16]. Based on 16S rRNA gene similarities using EzBioCloud [17], the threshold value for assignment to this family is approximately 91 %, which is close to the median sequence identity of 92.25 % reported for families but clearly above the threshold sequence identity of 86.5 % proposed by Yarza et al [18] for family demarcation.…”

“… Neisseriaceae species analysed for chemotaxonomic traits share the presence of a quinone system with ubiquinone Q-8 predominating, the major fatty acids C 16 : 0 and C 16 : 1 ω7 c , and the presence of the hydroxylic acid C 12 : 0 3OH. The presence of other hydroxyl acids is variable [1–5, 11, 13, 19–22]. In the polar lipid profiles, the phospholipids diphosphatidylglycerol, phosphatidylglycerol and phosphatidylethanolamine are usually predominant.…”

“…ursilacus [3], C. sinensis [4] and ‘ Populibacter cortices ’ [43]. Hence, it is supposed that the closely related species of strain 26B T have similar polar lipid profiles.…”

“…Unlike the well-known human pathogens Neisseria meningitidis and Neisseria gonorrhoeae, Neisseriaceae species are considered apathogenic or do not cause severe diseases. Species have been isolated from a wide diversity of habitats such as human sputum, oral cavities of sheep, cats and dogs, rectal content of Tibetan Plateau pika, gut of the Western honeybee, water of a karst cave, water of a crater lake, sediment lake water, laboratory tap water, sample of a hot spring, and soil of an ant hill [1][2][3][4][5][6][7][8][9][10][11][12][13][14][15][16]. Neisseriaceae species analysed for chemotaxonomic traits share the presence of a quinone system with ubiquinone Q-8 predominating, the major fatty acids C 16 : 0 and C 16 : 1 ω7c, and the presence of the hydroxylic acid C 12 : 0 3OH.…”