Undibacterium squillarum sp. nov., isolated from a freshwater shrimp culture pond

Abstract

A bacterial strain designated T was isolated from a freshwater shrimp culture pond inTaiwan and characterized using a polyphasic taxonomic approach. Cells of strain CMJ-15 T were facultatively anaerobic, Gram-stain-negative and motile by a single polar flagellum and formed light-yellow colonies. Growth occurred at 20-40 6C (optimum, 25-30 6C), with 0-0.7 % NaCl (optimum, 0-0.2 %) and at pH 5.0-9.0 (optimum, pH 6.0-7.0). Phylogenetic analyses based on 16S rRNA gene sequences showed that strain CMJ-15 T belonged to the genus Undibacterium and its closest neighbour was Undibacterium oligocarboniphilum EM 1 T , with sequence similarity of 96.3 %. The predominant cellular fatty acids were summed feature 3 (C 16 : 1 v7c and/or C 16 : 1 v6c), C 16 : 0 and C 18 : 1 v7c. The major cellular hydroxy fatty acid was C 10 : 0 3-OH. The polar lipid profile consisted of phosphatidylethanolamine, phosphatidylglycerol and diphosphatidylglycerol. The polyamine profile was composed of the major compound putrescine and moderate amounts of 2-hydroxyputrescine. The major respiratory quinone was Q-8 and the DNA G+C content was 52.2 mol%. On the basis of these phylogenetic and phenotypic data, strain CMJ-15 T should be classified as representing a novel species, for which the name Undibacterium squillarum sp. nov. is proposed. The type strain is CMJ-15 T (5BCRC 80404 T 5LMG 26892T 5KCTC 23917 T ).

Detection of bacterial endosymbionts in freshwater crustaceans: the applicability of non-degenerate primers to amplify the bacterial 16S rRNA gene

“…1). Undibacterium is a genus of typically aquatic bacteria, isolated from various sources of freshwater, e.g., drinking water, purified water, waterfalls, freshwater streams, freshwater shrimp culture ponds and also from permafrost soil (Kämpfer et al, 2007; Eder et al, 2011; Liu et al, 2013; Kim et al, 2014; Sheu et al., 2014a; Sheu et al., 2014b; Du et al., 2015; Nguyen, Lee & Kim, 2015; Chen et al., 2017). In addition, it was isolated from shrimp intestines (Rungrassamee et al, 2014) and from zebrafish Danio rerio (Kämpfer et al, 2016).…”

Elucidation of health risks using metataxonomic and antibiotic resistance profiles of microbes in flood affected waterbodies, Kerala 2018

“…Analysis of the Periyar river detected an increase in the Undibacterium of Burkholderiaceae family PF (Figure 7). The bacterium is generally found in drinking water and has also been isolated from soil, freshwater streams and ponds (Chen, Hsieh, Young, & Sheu, 2017; Kim et al, 2014; Sheu, Lin, Chen, & Chen, 2014). The order Betaproteobacteriales was significantly increased in the IAF scenario (65.96%) and is reported to be dominant in the oligotrophic groundwater ecosystem as well as freshwater habitats.…”

Undibacterium crateris sp. nov., isolated from water of crater lake

“…[3]. To date, the genus Undibacterium consists of 13 species isolated from water [1, 3–7], soil [7–9], aquatic animals [10, 11] and human intestinal tract [12]. The genus is characterized by Gram-stain-negative, oxidase-positive, non-spore-forming, rod-shaped bacteria with a flagellum.…”

“…The genus Undibacterium was stablished by Kämpfer et al with the description of the type species Undibacterium pigrum [1], as a member of the family Oxalobacteraceae, order Burkholderiales, class Betaproteobacteria [2], and subsequently emended by Eder et al [3]. To date, the genus Undibacterium consists of 13 species isolated from water [1,[3][4][5][6][7], soil [7][8][9], aquatic animals [10,11] and human intestinal tract [12]. The genus is characterized by Gram-stain-negative, oxidasepositive, non-spore-forming, rod-shaped bacteria with a flagellum.…”