Telah dilakukan penelitian secara kualitatif terhadap pengaruh antibakteri dari ekstrak kulit batang matoa (Pometia pinnata. Spp.) terhadap bakteri Gram positif Staphylococcus aureus . Sebelum dilakukan uji antibakteri, sampel yang telah diekstrak secara maserasi diuji fitokimia terlebih dahulu untuk menentukan kandungan metabolit sekunder yang telah diketahui berperan sebagai agen antibakteri. Setelah dilakukan uji fitokimia, ekstrak diuji aktivitas antibakterinya terhadap bakteri Staphylococcus aureus dengan menggunakan teknik difusi agar dengan cara sumuran. Ekstrak dilarutkan pada aquades steril dengan perbandingan 2 g ekstrak pada 2 mL air. Untuk kontrol positif, digunakan ciprofloxacin dan aquades steril sebagai kontrol negatif. Hasil penelitian ini menunjukkan bahwa ekstrak kulit batang matoa memiliki aktivitas antibakteri setelah diinkubasi selama 24 jam pada 37 OC. Dari 3 kali pengulangan dengan masing – masing 3 sumuran, didapat zona hambat masing – masing 16.84 mm, 12.5 mm dan 14.5 mm dengan kontrol positif 29.67 mm serta kontrol negatif 0 mm. Hasil yang diperoleh didukung oleh keberadaan metabolit sekunder hasil uji fitokimia yaitu tanin, flavonoid, terpenoid dan saponin.A qualitative study has done of the antibacterial effect of matoa (Pometia pinnata) stem bark extract against Gram-positive bacteria Staphylococcus aureus . Before the antibacterial test , samples were extracted by maceration and then phytochemical tested to measuring the content of secondary metabolites that have been known to act as an antibacterial agent . After being tested of phytochemical , extracts were tested the antibacterial effect against Staphylococcus aureus using agar diffusion technique. Extract was dissolved in sterile distilled water with a ratio of 2 g of extract in 2 mL of water . For the positive control , use of ciprofloxacin and sterile distilled water as a negative control . Results of this study indicate that matoa bark extract has antibacterial effect after incubation for 24 h at 37OC . Of 3 times with each repetition - each 3 wells, the inhibition zone obtained - each 16.84 mm , 12.5 mm and 14.5 mm with 29.67 mm of positive control and a negative control by 0 mm . The results are supported by the presence of secondary metabolites by phytochemical test such as tannins , flavonoids , terpenoids and saponins.
Penelitian ini bertujuan untuk menguji total kandungan flavonoid pada beberapa tanaman obat tradisional. Penentuan kandungan total flavonoid dilakukan dengan menggunakan metode etanol – HCl, sedangkan analisis flavonoid menggunakan Metode spektrofotometeyr UV-VIS . Hasil yang diperoleh menunjukan kandungan total flavonoid pada tanaman ketepeng cina (Cassia alata L) sebesar 26.8633 mg/mL , iler (Coleus scutellariodes L Benth) sebesar 14.425 mg/mL , rumput teki (Cyperus rotundus L) sebesar 6.505 mg/mL, pegagan (Centella asiatica) sebesar 3.816 mg/mL, rumput mutiara (Oldenlandia corymbosa) sebesar 2.686 mg/mL dan waru (Hibiscus tiliaceus L) sebesar 1.425 mg/mL. Kandungan total flavonoid tertinggi terdapat pada daun ketepeng sebesar 26.863 mg/mL, sedangkan kandungan flavonoid terendah terdapat pada daun waru sebesar 1.425 mg/mL. Analisis flavonoid dilakukan pada 𝜆 = 200 - 400 nm dan 𝜆maks = 205 nm. Hasil analisis flavonoid menunjukkan bahwa [A] daun iler panjang gelombang maksimum yaitu 205 nm dan (pita I) 300 nm dan (pita II) 250 nm dengan absorbansi 0.242 positif mengandung flavonol. [B] daun rumput mutiara panjang gelombang maksimum 205 nm dan dapat dilihat pada (pita I) 305 nm dan (pita II) 260 nm dengan absorbansi 0.023 positif mengandung flavonol. [C]) daun ketepeng panjang gelombang maksimum 205 nm dan dapat dilihat pada (pita I) 330 nm dan (pita II) 276 nm dengan absorbansi 0.167 positif mengandung flavonol. [D]) daun pegagan panjang gelombang maksmum 205 nm dan dapat dilihat pada (pita I) 310 nm dan (pita II) 265 nm dengan absorbansi 0.047 positif mengandung flavonol. (E) rumput teki hanya terdapat (I pita) yaitu 295 nm dengan absorbansi 0.029 positif mengandung flavon. (F) Begitupun dengan daun waru hanya terdapat (I pita) yaitu 290 nm dengan absorbansi 0.036 positif mengandung flavon. Dapat disimpulkan bahwa berdasarkan uji kualitatif tanaman iler, ketepeng, rumput mutiara, rumput teki dan pegagan mengandung flavonoid kecuali tanaman waru tidak terdeteksi kandungan flavonoidnya.This studyaims to examine the total flavonoid contentin some traditional medicinal plants. Determination of total flavonoid content swere determined using ethanol-HCl, where as flavonoid analysis was analysed by spectrophotometry UV-VIS.The results showed the total flavonoid contentin Ketepeng China plant (Cassia alataL.) was 26.863 mg/ml, Iler plant (Coleus scutellariodes L. Benth) was 14.2464 mg/ml, nut-grass (Cyperus rotundus L.) was 6.505 mg/ml; Pegagan (Centella asiatica) was 3.816 mg/ml; pearl grass (Oldenlandia corymbosa) was 2.686 mg/ml, and Waru (Hibiscus tiliaceus L.) was 1.425 mg/ml. The highest total flavonoid was found in ketepeng(26.8633mg/ml), whereas the lowest was found in waru (1.4246 mg/ml). Flavonoid analysis was performed using a spectrometer with a wavelength (λ) of 200-400 nm, with λmax = 205 nm. Flavonoid analysis using a spectrophotometer, amon others showed that: (1) The maximum wavelength of Iler leaf was 205 nm and can be see non the Band I 300 nm and Band II 250 nm, with 0.242 absorbance. This indicates that this isolate was positive for flavonols; (2) Maximum wavelength of pearl leaf was 205 nm and can be see non the Band I 305 nm and Band II 260 nm, with 0.023 absorbance. This indicates that this isolate was positive for flavonols; (3) Maximum wavelength of ketepeng leaf was 205 nm and can be see non the Band I 330 nm and Band II 276 nm, with 0.167 absorbance. This indicates that this isolate was positive for flavonols; (4) Maximum wavelength of pegagan leaf was 205 nm and can be see non the Band I 310 nm and Band II 265 nm, with 0.047 absorbance. This indicates that this isolate was positive for flavonols; (5) nut-grass had only 1 band, namely 295 nm with absorbance 0.029. This indcates that this isolate was positive for flavons; (6) waru leaf had also only 1 band, namely 290 nm with absorbance 0.036. This this isolate was positive for flavons.
Telah dilakukan penelitian tentang skrining fitokimia dan uji aktivitas antioksidan dari daun nasi (Phrynium capitatum) dengan metode DPPH (1,1-difenil-2-pikrilhidrazil). Skrining fitokimia meliputi pengujian alkaloid, flavonoid, saponin, steroid, triterpenoid dan fenolik. Uji aktivitas antioksidan dilakukan dengan penambahan 2 mL larutan DPPH dengan 0,5 mL larutan ekstrak dengan konsentrasi masing-masing 1000, 750, 500 dan 250 µg/mL. Data yang diproleh kemudian digunakan untuk menghitung nilai IC50 dari ekstrak etanol daun nasi. Hasil pengujian menunjukan bahwa ekstrak etanol daun nasi mengandung senyawa alkaloid, flavonoid, saponin, steroid dan fenolik. Ekstrak etanol daun nasi memiliki aktivitas antioksidan trrtinggi pada konsentrasi 1000 µg/mL sebesar 65,77 % dan semakin menurun aktivitasnya dengan berkurangnya konsentrasi ekstrak, konsentrasi 750, 500 dan 250 µg/mL masing-masing sebesar 53,96 %, 43,8 % dan 19,58 % dengan nilai IC50 pada konsentrasi 701,78 µg/mL.A research on phytochemicals screening and antioxidant activity test of daun nasi (Phrynium capitatum) using DPPH (1,1-diphenyl-2-picrylhydrazyl) method had been conducted. Test for alkaloids, flavonoids, saponins, steroids, triterpenoids and phenolic were included in phytochemical screening. Antioxidant activity was tested through the addition of 2 mL DPPH solution into 0.5 mL of the extract having a concentration of 1000, 750, 500 and 250 µg/mL respectively. The data obtained was used to calculate the IC50 value for the ethanol extract of daun nasi. The results showed that the ethanol extract of daun nasi contains alkaloids, flavonoids, saponins, steroids and phenolic. Ethanol extract of daun nasi has the highest antioxidant activity of 65.77% at concentration of 1000 µg/mL. Its activity decreased with decreasing concentration of the extract, which were 53.96 %, 43.8 % and 19.58 % at concentration of 750, 500 and 250 µg/mL respectively. IC50 values was found at a concentration of 701.78 µg/mL.
Composite resin is one of the restoration material which has the advantage in aesthetic due to its colour. This make the composite resin as a material of choice, unfortunately most of patients who have received composite resin treatment doesn’t have a proper careness to its own restoration after treatment. Aim: to study how many people who use composite resin as restoration material and to study if the restoration has any leakage after treatment. Results: the study showed that women have more composite resin restoration than men. The leakage occurred to three different subject in this research which is the most leakage happened to 2007’s student, followed by 2006’s and 2005’s. Class one Black classification is the most restoration to be found in this study followed by class 4, class 5, class 3, and class 2. The leakage which found in this study mostly occurred to molar restorations, followed by premolars while incicivus was found to be the least. Keywords: resin, composite, leakage, restoration. Abstrak: Resin komposit merupakan salah satu jenis bahan tumpatan yang memiliki keunggulan dalam bidang estetik karena merupakan bahan tumpatan yang sewarna dengan gigi. Hal ini menjadikan resin komposit sebagai bahan pilihan restorasi, namun banyak pasien yang telah mendapatkan perawatan restorasi resin komposit kurang memperhatikan tumpatan pasca restorasi tersebut. Tujuan: penelitian ini bertujuan untuk mengetahui gambaran penggunaan resin komposit sebagai bahan tumpatan gigi pada restorasi kavitas dan mengetahui apakah telah terjadi kebocoran tepi tumpatan resin komposit pada perawatan restorasi kavitas. Hasil penelitian: menunjukkan distribusi penggunaan komposit lebih banyak pada perempuan dibandingkan laki-laki. Terjadi kebocoran tumpatan pada subjek penelitian dari tiga angkatan yang berbeda yaitu terbanyak pada mahasiswa angkatan 2007 kemudian 2006 dan 2005. Tumpatan kavitas kelas I merupakan tumpatan yang paling banyak diikuti kelas 4, kelas 5, kelas 3, dan kelas 2. Kebocoran gigi yang ditemukan pada penelitian ini terjadi paling banyak pada gigi molar kemudian premolar sedangkan insisivus paling sedikit. Kata kunci: resin, komposit, kebocoran, restorasi.
Telah dilakukan penelitian untuk menganalisis kualitas air limbah yang berasal dari Pertambangan Emas Tanpa Izin (PETI) di Desa Bakan Kecamatan Lolayan Kabupaten Bolaang Mongondow. Parameter yang diukur adalah pH, padatan tersuspensi total (TSS), tembaga, kadmium, zink, timbal, arsen, nikel, kromium, merkuri, dan sianida. Pengambilan sampel dilakukan pada empat lokasi di sepanjang aliran air limbah buangan dari lokasi pertambangan emas. Nilai hasil pengukuran selanjutnya dibandingkan dengan Keputusan Menteri Negara Lingkungan Hidup No. 202 Thn. 2004 tentang Baku Mutu Air Limbah Bagi Kegiatan Penambangan Bijih Emas/Tembaga dan Keputusan Menteri Negara Lingkungan Hidup Republik Indonesia Nomor 5 Thn. 2014 tentang Baku Mutu Air Limbah. Hasil penelitian menunjukkan bahwa nilai pH di satu lokasi pengambilan sampel dan nilai TSS di dua lokasi pengambilan sampel berada di luar nilai baku mutu yang telah ditetapkan, sedangkan konsentrasi sianida dan semua logam yang dianalisis masih berada di bawah ambang batas yang ditetapkan.A research aimed to analyze quality of wastewater from Unlicensed Gold Mine in Bakan Village Lolayan District Bolaang Mongondow Regency has been conducted. Parameters examined were pH, total suspended solid (TSS), copper, cadmium, zinc, lead, arsenic, nickel, chromium, mercury, and cynide. Sampling was performed at four locations along the flow of wastewater sourced from unlicensed gold mine. The results were then compared to Decree of the Minister of State for the Environment No. 202 Yrs. 2004 on the Quality Standard of Waste Water from Gold/Copper Ore Mining Activities and Decree of the Minister of State for the Environment No. 5 Yrs. 2014 on the Quality Standard of Waste Water. The results showed that pH value at one location of sampling and TSS value at two locations of sampling were beyond the predefined quality standard, while the concentration of cyanide and all metals analyzed were below the prescribed threshold.
scite is a Brooklyn-based organization that helps researchers better discover and understand research articles through Smart Citations–citations that display the context of the citation and describe whether the article provides supporting or contrasting evidence. scite is used by students and researchers from around the world and is funded in part by the National Science Foundation and the National Institute on Drug Abuse of the National Institutes of Health.
customersupport@researchsolutions.com
10624 S. Eastern Ave., Ste. A-614
Henderson, NV 89052, USA
This site is protected by reCAPTCHA and the Google Privacy Policy and Terms of Service apply.
Copyright © 2024 scite LLC. All rights reserved.
Made with 💙 for researchers
Part of the Research Solutions Family.