ABSTRAKIkan nila atau Oreochromis niloticus merupakan ikan konsumsi masyarakat di Indonesia. Kasus kematian massal ikan nila terjadi di beberapa lokasi budaya di Jawa, Lombok, dan Sumatera yang disebabkan oleh infeksi Orthomyxovirus, dan disebut sebagai Tilapia Lake Virus (TiLV). Tujuan penelitian ini adalah untuk mendeteksi adanya infeksi TiLV dengan metode semi-nested Reverse Transcriptase-Polymerase Chain Reaction (RT-PCR) pada kasus kematian massal ikan nila. Lokasi pengambilan sampel di Desa Sigerongan Kecamatan Lingsar, Lombok, Nusa Tenggara Barat. Analisis deteksi RT-PCR menggunakan sampel organ otak, ginjal, limpa, dan hati, selanjutnya dilakukan sekuensing. Hasil pengamatan terhadap gejala klinis terhadap ikan nila moribund terlihat kondisi mata yang buram/katarak, serta cekung, abrasi kulit, serta perubahan warna tubuh menjadi lebih gelap. Hasil analisis RT-PCR menunjukkan bahwa kejadian kematian massal pada ikan nila suspektif diakibatkan oleh infeksi RNA virus TiLV. Analisis sekuensing menunjukkan bahwa TiLV dari sampel ikan nila di Lombok mempunyai kesamaan identitas genetik 97% dengan TiLV asal Israel (Genebank Accession Number KU 751816.1).
KATA KUNCI: nila; TiLV; seminested RT-PCR
ABSTRACT:Case study of tilapia lake virus (TiLV) infection on nila (Oreochromis niloticus
<strong>Heavy Metal Accumulation on Green Mussels in the Java Coastal Water.</strong>In many countries, green mussel (<em>Pernaviridis</em>) is harvested as food source. It is also commonly used as indicators for toxic contaminant, i.e. heavy metals, in the coastal waters. In Indonesia, green mussels are mostly cultivated in the north coast of Java where most industrial estates are located. Without further treatment, toxic contaminant of industrial waste may give impact to the surrounding environment, not to mention the adjacent coastal waters. This study was aimed to determine the accumulation of heavy metals, namely Hg, Pb and Cd, in green mussels. Green mussel samples were collected in 2017, taken from four culture sites in the north coast of Java: Panimbang, Jakarta Bay, Brebes and Cirebon. Our results showed that the concentration of Hg, Pb and Cd in green mussel tissues of those locations are: Panimbang = Hg: <0.22 mg/kg, Pb: undetectable, and Cd: 0.068 mg/kg; Teluk Jakarta = Hg: 11.7 mg/kg, Pb: 29.4 mg/kg, and Cd: 0.42 mg/kg; Brebes = Hg: 0.01 mg/kg, Pb: 3.52 mg/kg, and Cd: 0.4 mg/kg; and Cirebon = Hg: 0.01 mg/kg, Pb: 2.66 mg/kg, and Cd: 0.73 mg/kg. These results indicate that only green mussels from Panimbang contain heavy metals below the thresholds allowed to be consumed by humans
ABSTRACT-Fingerling humpback grouperCromileptes altivelis, experimentally infected with a betanodavirus (RGNNV genotype), were kept at 27∞C, 31∞C or 35∞C for 14 days. The numbers of dead fish in ten fish at each water temperature were five at 27∞C, two at 31∞C and one at 35∞C. The coat protein gene of the virus was detected by RT-PCR from four survivors at 27∞C, two survivors at 31∞C, but not from survivors at 35∞C. Histopathologically, vacuolation in the retinal tissues was observed in three survivors at 27∞C, but not in survivors at 31∞C or 35∞C. These results suggest that high water temperature inhibits the viral proliferation in fish.
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