Immune response of humpback grouper, <i>Cromileptes altivelis</i> (Valenciennes) injected with the recombinant coat protein of betanodavirus

Yuasa, Kei; Koesharyani, Isti; Roza, Des; Mori, Koichiro; Katata, Masahide; Nakai, Toshiko

doi:10.1046/j.1365-2761.2002.00325.x

Cited by 59 publications

(26 citation statements)

References 14 publications

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“…Intramuscular injection of the recombinant RGNNV coat protein, which was expressed in Escherichia coli, induced neutralizing antibodies in the fish Epinephelus septemfasciatus and Chromileptes altivelis, and induced protection against challenge by RGNNV genotype viruses (Tanaka et al 2001, Yuasa et al 2002. A similar vaccine efficacy was obtained in juvenile turbot Scophthalmus maximus by intraperitoneal injection of an oil-emulsified recombinant coat protein from SJNNV (Húsgard et al 2001).…”

mentioning

confidence: 70%

“…We used 16 other betanodavirus isolates from diseased fishes in neutralization tests with the antisera (see Table 1). These, except JFHir96, were used in our previous studies (Iwamoto et al 1999, Curtis et al 2001, Tanaka et al 2001, Yuasa et al 2002. Fish-pathogenic infectious pancreatic necrosis virus (IPNV; VR299 strain), infectious hematopoietic necrosis virus (IHNV; ChAb strain) and viral hemorrhagic septicemia virus (VHSV; Obama25 strain) were also used as reference viruses for neutralization tests.…”

Section: Virus Isolatesmentioning

confidence: 99%

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Serological relationships among genotypic variants of betanodavirus

Mori¹,

Mangyoku²,

Iwamoto³

et al. 2003

Dis. Aquat. Org.

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Betanodaviruses, the causative agents of viral nervous necrosis or viral encephalopathy and retinopathy, are divided into 4 genotypes based on the coat protein gene (RNA2). In the present study, serological relationships among betanodavirus genotypic variants were examined by virus neutralization tests using rabbit antisera raised against purified virions of strains representative of each genotype. All 20 isolates examined shared epitopes for neutralizing, but they fell into 3 major serotypes (A, B, C). This sero-grouping is in part consistent with their genotypes, i.e. Serotype A for striped jack nervous necrosis virus (SJNNV) genotype, Serotype B for tiger puffer nervous necrosis virus (TPNNV) genotype, and Serotype C for both redspotted grouper nervous necrosis virus (RGNNV) and barfin flounder nervous necrosis virus (BFNNV) genotypes. The serological relatedness between RGNNV and BFNNV genotypes may result from their relatively higher similarity in RNA2 sequences. In neutralization tests using antisera of kelp grouper Epinephelus moara, which were raised against recombinant coat proteins representing each genotype, anti-SJNNV and anti-TPNNV sera neutralized only the homologous strain, and anti-RGNNV and anti-BFNNV sera reacted with both RGNNV and BFNNV strains. The present serological findings will be important in investigating the infectivity and host-specificity of betanodaviruses and in developing vaccines for the disease.

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mentioning

confidence: 70%

Section: Virus Isolatesmentioning

confidence: 99%

Serological relationships among genotypic variants of betanodavirus

Mori¹,

Mangyoku²,

Iwamoto³

et al. 2003

Dis. Aquat. Org.

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“…To ensure that the fish are as healthy as possible, a useful and practical approach would be vaccination of fish and boosting their immune system. Various types of vaccines have been developed such as formalininactivated vaccine, subunit vaccine with recombinant coat protein of betanodavirus and nodavirus-like particle vaccine [58,74,111,116,118,121,124]. Vaccination trials have shown protection efficacy against betanodavirus infection.…”

Section: Disease Control Strategiesmentioning

confidence: 99%

Betanodavirus of Marine and Freshwater Fish: Distribution, Genomic Organization, Diagnosis and Control Measures

et al. 2012

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“…Under these circumstances, several kinds of fish nodavirus vaccines have been developed, such as formalin-inactivated vaccine, subunit vaccine with a recombinant coat protein, virus-like particle vaccine, etc. (Húsgar7 et al 2001, Tanaka et al 2001, Yuasa et al 2002, Sommerset et al 2005, Yamashita et al 2005, Liu et al 2006, Thiéry et al 2006.…”

Section: Introductionmentioning

confidence: 99%