AbstractWe aim to analyze in bounds KJ Tracey’s immunological homunculus conception the relationships between parameters of electroencephalogram (EEG) and heart rate variability (HRV), on the one hand, and the parameters of bhite blood cell count, on the other hand.MethodsIn basal conditions in 23 men, patients with chronic pyelonephritis and cholecystitis in remission, recorded EEG (“NeuroCom Standard”, KhAI Medica, Ukraine) and HRV (“Cardiolab+VSR”, KhAI Medica, Ukraine). In portion of blood counted up white blood cell count.ResultsRevealed that canonical correlation between constellation EEG and HRV parameters form with blood level of leukocytes 0.92 (p<10-5), with relative content in white blood cell count stubnuclear neutrophiles 0.93 (p<10-5), segmentonucleary neutrophiles 0.89 (p<10-3), eosinophiles 0.87 (p=0.003), lymphocytes 0.77 (p<10-3) and with monocytes 0.75 (p=0.003).ConclusionParameters of white blood cell count significantly modulated by electrical activity some structures of central and autonomic nervous systems.

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Background. Previously, we found a wide range of uric acid exchange parameters and functional relationships of uricemia and uricosuria with the parameters of immunity in healthy rats analyzed. The purpose of this study is to clarify such relationships in patients with neuroendocrine-immune complex dysfunction on the background of chronic pyelonephritis combined with cholecystitis in remission. Material and Methods. The object of observation were 34 men and 10 women aged 24-70 years old, who came to the Truskavets' spa for the rehabilitation treatment. The serum and daily urine levels of the uric acid by uricase method were determined. Immune status evaluated on a set of I and II levels recommended by the WHO. The condition of microbiota is evaluated on the results of sowing of feces and urine. Results. The canonical correlation analysis revealed that raw uricemia determines by 28% nine parameters of immunity (relative blood content of pan-lymphocytes and their CD4 +-, CD56 +-, 0-populations, relative content of polymorphonuclear neutrophils, intensity and completeness of their phagocytosis Staph. aureus and their bactericidal capacity, saliva content of IgG) as well as bacteriuria and content in E. coli feces. Uricemia, normalized by sex and age, determines by 25% another constellation of immunity parameters (relative CD8 + lymphocytes content, CIC, E. coli phagocytosis intensity and completeness, Staph. aureus phagocytosis activity and completeness) as well as content in E. coli feces with impaired enzymatic activity and Klebsiela&Proteus. Instead, uricosuria determines only four parameters of immunity and only by 11,5%. Conclusion. Endogenous uric acid has a modulating overall beneficial effect on a number of immune and microbiota parameters in both healthy rats and people with neuroendocrine-immune complex dysfunction.

Background. Previous experimental and clinical-physiological studies have shown that the Ukrainian phytocomposition “Balm Kryms’kyi” has adaptogenic properties, which are manifested in its vegetative, endocrine, immunotropic, coagulotropic, actotropic and metabolic effects. However, the neurotropic properties of the phytocomposition remain unclear, which is the purpose of this study. Materials and methods. The object of observation were 12 women (44±13 years) and 62 men (44±12 years) with dysfunction of neuro-endocrine-immune complex. HRV and EEG parameters were recorded in the morning in basal conditions. Then the members of the main group used 5 ml of phytocomposition dissolved in 45 ml of tap water, instead in the control group used 50 ml of the latter. After 1,5 hours, the test was repeated. Results. Discriminant analysis revealed 30 EEG parameters and 4 HRV parameters characteristic of the initial state and after consumption of phytocomposition or tap water. Conclusion. Ukrainian phytocomposition “Balm Truskavets” causes immediate neurotropic effects at patients with dysfunction of neuro-endocrine-immune complex.

Relationships between changes in uric acid parameters metabolism and parameters of immunity and microbiota in patients with neuroendocrine-immune complex dysfunction.

zukow@umk.pl 4 OO Bohomolets' Institute of Physiology, Kyїv i.popovych@biph.kiev.ua AbstractBackground. Previously, we found a wide range of uric acid metabolism parameters and functional relationships of uricemia and uricosuria with the parameters of immunity in healthy female rats analyzed. It was found by canonical correlation analysis that both parameters of uric acid metabolism determine the immunity status of rats by 71%. The purpose of this study is to determine the features of immune status in rats with quantitatively and qualitatively different uric acid metabolism. Material and Methods. Experiment was performed on 60 healthy female Wistar rats 220-300 g. Among them 10 animals remained intact, using tap water from drinking ad libitum. The rats of others groups for 6 days administered through the tube various fluids. The serum and urine levels of the uric acid (uricase method) were determined. In the blood, the parameters of immunity were determined. From thymus and spleen made smears-imprints for counting splenocytogram and thymocytogram. For them, as well as immunocytogram and leukocytogram of blood, Shannon's entropy was calculated. Results. Screening of immune profiles of four quantitative and qualitative uric acid metabolism clusters revealed 6 functionalmetabolic patterns, two of which are quasilinear (enhancing and suppressing), with immune extremes at moderately elevated uricemia, two U-shaped with immune optimum at moderately reduced uricemia levels, as well as a non-reactive pattern. Discriminant analysis revealed 14 parameters of immunity, the constellation of which four clusters of metabolism of uric acid clearly distinguish. Conclusion. Endogenous uric acid exerts a modulatory immunotropic effect in healthy female rats.168

Background. It was recently shown by our group that bilirubin has a neurotropic activity. It is also shown that endogenous uric acid exerts a noticeable modulating effect on neuro-endocrine adaptation factors. In this study, we supplemented the constellation both of subjects of influence with other nitrogenous metabolites - urea and creatinine, and the objects of influence - with plasma levels of the main adaptation hormones and the severity of trait and reactive anxiety. Materials and Methods. The object of observation were almost healthy volunteers: 30 females (30÷76 y) and 31 males (24÷69 y). In basal conditions determined plasma levels of nitrogenous metabolites as well as cortisol, aldosterone, testosterone, triiodothyronine and calcitonin, estimated the severity of the trait and reactive anxiety, recorded the ongoing HRV and EEG. After 4 or 7 days, repeated testing was performed. Results. By constructing regression models, it was found that direct bilirubin determines the levels of psycho-neuro-endocrine parameters by 66,0%, free bilirubin by 56,7%, uric acid by 40,8%, creatinine by 37,6%, and urea - by 31,1%. Taken together, nitrogenous plasma metabolites determine the severity of trait, but not reactive anxiety, plasma levels of testosterone, cortisol and triiodothyronine, but not calcitonin and aldosterone, as well as a number of HRV and EEG parameters by 70,6%. Conclusion. Nitrogenous plasma metabolites, even in the absence of uremia, are able to influence the state of the psyche, autonomic and central nervous and endocrine systems, apparently through aryl hydrocarbon and adenosine receptors of neurons and endocrinocytes and/or directly.

Background. Earlier have been shown that the immune responses to course of drinking of Naftussya bioactive water from Truskavet's spa are ambiguous and individual. However, at Truskavets' spa water monotherapy is a rare exception for specific contingents, whereas the vast majority of patients use a balneotherapy complex: drinking of Naftussya, application of ozokerite and mineral baths. The immune responses to balneotherapeutic complex are also ambiguous which is a separate manifestation of the multivariate effects of balneological agents as well stressors on the body. Therefore, the purpose of this study is to analyze variants of immune responses to balneotherapeutic complex of Truskavets' spa. Material and methods. The object of observation were 34 men and 10 women aged 24-70 years old, who came to the Truskavets' spa for the treatment of chronic pyelonephritis combined with cholecystitis in remission. The survey was conducted twice, before and after balneotherapy (drinking Naftussya bioactive water three times a day, ozokerite applications, mineral baths every other day for 7-10 days). Immune status evaluated on a set of I and II levels recommended by the WHO. In portion of capillary blood we counted up Leukocytogram and calculated two variants of Adaptation Index as well as two variants of Strain Index by IL Popovych. We calculated also the Entropy of Immunocytogram and Leukocytogram. The condition of Microbiota is evaluated on the results of sowing of feces and urine. Results. Four variants of the immune responses to balneotherapeutic complex have been identified. In 40,9% of patients, initially normal immune status did not change significantly. In 31,8%, the lower boundary level of immunity is completely normalized. In 22,7% moderate immunosuppression is reduced, but not up to normal. However, in 4,5% of people, initially normal level of immunity are transformed into moderate immunosuppression. Discriminant analysis was conducted to identify exactly the parameters of the immunity and microbiota, in which the four immune response clusters differ significantly from each other. 24 parameters were characteristic, 12 of them related to the immune parameters of the blood, one of the saliva, 5 of the feces microbiota and 3 related to urinary syndrome as well as 4 parameters are information. The other 25 parameters were outside the discriminatory model. Conclusion.