Background. Previously, we found a wide range of uric acid exchange parameters and functional relationships of uricemia and uricosuria with the parameters of immunity in healthy rats analyzed. The purpose of this study is to clarify such relationships in patients with neuroendocrine-immune complex dysfunction on the background of chronic pyelonephritis combined with cholecystitis in remission. Material and Methods. The object of observation were 34 men and 10 women aged 24-70 years old, who came to the Truskavets' spa for the rehabilitation treatment. The serum and daily urine levels of the uric acid by uricase method were determined. Immune status evaluated on a set of I and II levels recommended by the WHO. The condition of microbiota is evaluated on the results of sowing of feces and urine. Results. The canonical correlation analysis revealed that raw uricemia determines by 28% nine parameters of immunity (relative blood content of pan-lymphocytes and their CD4 +-, CD56 +-, 0-populations, relative content of polymorphonuclear neutrophils, intensity and completeness of their phagocytosis Staph. aureus and their bactericidal capacity, saliva content of IgG) as well as bacteriuria and content in E. coli feces. Uricemia, normalized by sex and age, determines by 25% another constellation of immunity parameters (relative CD8 + lymphocytes content, CIC, E. coli phagocytosis intensity and completeness, Staph. aureus phagocytosis activity and completeness) as well as content in E. coli feces with impaired enzymatic activity and Klebsiela&Proteus. Instead, uricosuria determines only four parameters of immunity and only by 11,5%. Conclusion. Endogenous uric acid has a modulating overall beneficial effect on a number of immune and microbiota parameters in both healthy rats and people with neuroendocrine-immune complex dysfunction.
zukow@umk.pl 4 OO Bohomolets' Institute of Physiology, Kyїv i.popovych@biph.kiev.ua AbstractBackground. Previously, we found a wide range of uric acid metabolism parameters and functional relationships of uricemia and uricosuria with the parameters of immunity in healthy female rats analyzed. It was found by canonical correlation analysis that both parameters of uric acid metabolism determine the immunity status of rats by 71%. The purpose of this study is to determine the features of immune status in rats with quantitatively and qualitatively different uric acid metabolism. Material and Methods. Experiment was performed on 60 healthy female Wistar rats 220-300 g. Among them 10 animals remained intact, using tap water from drinking ad libitum. The rats of others groups for 6 days administered through the tube various fluids. The serum and urine levels of the uric acid (uricase method) were determined. In the blood, the parameters of immunity were determined. From thymus and spleen made smears-imprints for counting splenocytogram and thymocytogram. For them, as well as immunocytogram and leukocytogram of blood, Shannon's entropy was calculated. Results. Screening of immune profiles of four quantitative and qualitative uric acid metabolism clusters revealed 6 functionalmetabolic patterns, two of which are quasilinear (enhancing and suppressing), with immune extremes at moderately elevated uricemia, two U-shaped with immune optimum at moderately reduced uricemia levels, as well as a non-reactive pattern. Discriminant analysis revealed 14 parameters of immunity, the constellation of which four clusters of metabolism of uric acid clearly distinguish. Conclusion. Endogenous uric acid exerts a modulatory immunotropic effect in healthy female rats.168
Relationships between changes in uric acid parameters metabolism and parameters of immunity and microbiota in patients with neuroendocrine-immune complex dysfunction.
Background. Uric acid is traditionally considered as the final product of human and primate DNA/RNA degradation, devoid of useful physiological activity. However, there is an opinion that the uric acid molecule, by analogy with methylxanthines (caffeine, theophylin, theobromine) has physiological activity. The acquired experience allows us to offer a topic for future research: "Neurotropic and immunotropic activity of endogenous uric acid". Working hypothesis. Uric acid, interacting with A1 and A2a adenosine receptors as well as phosphodiesterase and Na,K-ATPase of neurons, modulates the activity of nerve centers, which in turn modulate immunocytes. Perhaps the direct effect of uric acid on immunocytes, since the existence of theophylline-resistant and theophylline-sensitive subpopulations of Tlymphocytes has long been known. The proposed article is the first swallow of the announced project. Material and Methods. Experiment was performed on 58 healthy female Wistar rats 220-300 g. Among them 10 animals remained intact, using tap water from drinking ad libitum.The rats of others groups for 6 days administered through the tube various fluids at a dose of 1,5 mL/100 g of body mass. The day after the completion of the drinking course the plasma
Background. Neonatal hyperbilirubinemia has been known to damage neural function. Our goal is to determine whether the neurotropic activity of normal bilirubinemia in adults is evident. Methods. The object of observation were 77 volunteers: 30 women and 47 men aged 49±13 (26 ÷ 76) years without clinical diagnosis. Testing was performed twice with an interval of 4 ÷ 10 days. We determined the plasma levels of the direct and free bilirubin, recorded EEG and HRV followed by analysis of correlations between parameters. Results. Significant downregulating effect of bilirubinemia was found on power spectrum density (PSD) theta and delta rhythm. In contrast, bilirubinemia has an upregulating effect on PSD beta rhythm and sympatho-vagal balance. The canonical correlation between direct & free bilirubin levels, on the one hand, and EEG & HRV parameters, on the other hand, is very strong: R=0,808; R2=0,654; χ2(80)=191; p<10-6 (n=154). A similar canonical correlation was found between individual changes in parameters: R=0,753; R2=0,568; χ2(48)=83; p=0,001 (n=74). Conclusion. Even normal bilirubinemia has an downregulating effect on mainly theta and delta rhythm-generating nuclei and vagal tone, while upregulating effects on sympathetic tone and beta rhythm-generating nuclei.
Артеріальна гіпертензія може суттєво погіршувати стан здоров'я вагітної та часто спричиняє ускладне ння в пологах та перинатальні ускладнення.Мета дослідження: виявити переваги та недоліки різних схем антигіпертензивної терапії у жінок з артеріальною гіпертензією під час процесу пологів та їх вплив на перебіг пологів та перинатальні результати.
Мета дослідження -з'ясувати характерні особливості структурних трансформацій ендометрія за умов коморбідності в жінок репродуктивного віку із первинним та вторинним непліддям.Матеріали та методи. Проведено аналіз медичних карт та патоморфологічного дослідження біопсій ендометрія жінок репродуктивного віку із діагностованим непліддям. Дизайн обстеження включав гінекологічне, загальноклінічні та профільні інструментальні й лабораторні дослідження, результати патоморфологічного дослідження. Вивчали дані анамнезу, репродуктивної функції, гінекологічних захворювань, соматичного статусу.Результати дослідження та їх обговорення. Усі жінки обстежені в умовах стаціонару. У 65,7 % пацієнток діагностовано первинне непліддя і в 34,3 % -вторинне. Патологічні зміни ендометрія, а саме залозиста гіперплазія, стромально поліпозна трансформація, ендометрити, зареєстровані у 55,6 % серед жінок із первинним непліддям та в 71,4 % -у жінок із вторинним непліддям. Частка ж невстановленого причинного фактора суттєво не відрізнялася в обох групах обстежуваних і становила 0,4 % і 0,6 %. Структура патологій функціонального шару матки при вторинному неплідді морфологічно відрізнялася ознаками більш вираженої проліферативної активності залозистих структур при залозистих гіперплазіях, залозистого і стромального компонентів при поліпах ендометрія та вираженістю проявів хронічного запалення, тоді як при первинному неплідді ознаки проліферативної активності ендометрія були менш вираженими, за винятком тих, що розвинулися за умов тиреопатій. Зважаючи на той факт, що, за результатами наших досліджень, у більшості жінок із вторинним непліддям (81,2 % обстежуваних) зустрічалася екстрагенітальна патологія та перевагу мали тиреопатії, вважаємо доцільним подальше поглиблене вивчення особливостей ендометріальної трансформації у жінок із непліддям та їх можливого зв'язку із порушеннями роботи щитоподібної залози.Висновки. Морфофункціональний стан слизової матки слід вважати ендометріальним фактором складової при плануванні вагітності. У структурі коморбідності у пацієнток із вторинною неплідністю переважає відсоток патології щитоподібної залози. У жінок із тиреопатіями переважно діагностуються проліферативна залозисто-стромальна поліпозна трансформація ендометрія, а також від двох до трьох випадків викиднів у різні терміни вагітності. До особливості ремоде-лювання ендометрія за умов поліморбідності доцільно віднести високу частоту залозисто-кістозної гіперплазії та залозисто-стромальної трансформації.Ключові слова: первинне непліддя; вторинне непліддя; репродуктивний вік; коморбідність; ендометрій.
<p>Background. Currently, miscarriage is considered to be a multietiological disorder with trombofilic violations<br />and hormone deficiency as the leading factors. Despite the achievements in treatment of miscarriage, the<br />frequency of preterm termination of the wanted pregnancies is still high and the number of perinatal losses is<br />significant. Therefore, pathogenetically based therapy, safe for the foetus, is very important in management of<br />pregnancy interruption in the first trimester. A proper drugs administration provides optimal concentration of<br />active ingredients and fast action. The aim is to improve effectiveness of the early threatened abortion treatment<br />in cases of subchorionic hematoma (SCH) by combination of sublingual natural micronized progesterone and<br />tranexamic acid<br />Objective. We examined 50 pregnant women with early threatened abortion with SCH. We studied system<br />of haemostasis, basic hormonal markers and ultrasound criteria of threatened abortion. We compared efficacy<br />of treatment between traditional (supportive) therapy (sedation, spasmolytic, haemostatic drug) and combination<br />of supportive therapy in combination with tranexamic acid and natural micronized progesterone.<br />Results. The result of lab tests showed minimal signs of hypercoagulation, hyperfibrinogenemia and platelet<br />hyperactivity, a significant β-hCG level decrease and approximate decrease in progesterone and free estriol<br />production.<br />Sonographic examination showed presents of local myometrial hypertonus, deformation of fertilized egg,<br />hypoplasia of chorion, low location of fertilized ovum, retarded growth of CRL.<br />The research proved that combined administration of sublingual micronized progesterone and tranexamic<br />acid for the treatment of threatened abortion with SCH has more significant positive effect for pregnancy<br />maintenance due to clinical, biochemical, hormonal and ultrasound results if compared with the group which<br />underwent supportive therapy.<br />Conclusions. Complex application of natural micronized progesterone 100 mg three times a day sublingually<br />and 500 mg of Tranexamic acid dissolved in 200 ml normal saline solution improves the dynamics of the<br />main hormonal, haemostatic and ultrasound markers of abortion and significantly reduces reproductive losses.<br />Tranexamic acid treatment proved a rapid and effective action on hematoma and absence of embryotoxical and<br />сoagulopathyc influence. Tranexamic acid does not cause any significant disorders of hemostatic system. This is<br />very important at the early gestation because of intravascular coagulation, physiological hypercoagulable condition<br />during pregnancy that can cause microthrombosis and disrupt placentation. On the other hand, it is<br />dangerous for the mother’s health because of the increased risk of thrombosis.<br />KEY WORDS: threatened miscarriage, subhorial hematoma, micronized progesterone, tranexamic<br />acid.</p>
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