This study aimed to investigate the effect of Rhodamine B exposure on oxidative stress and cervical epithelial cells proliferation in the uterus. Twenty eight female Wistar albino rats were divided into four groups (n = 7 each): one control (untreated) group; and three Rhodamine B groups at several doses (4.5, 9, 18 mg/200 g body weight/day) for 36 days. Colometric analysis of malondialdehyde (MDA) level as a marker of lipid peroxidation and histological analysis of the cervical epithelial cells proliferation was performed. The MDA levels and proliferation of epithelial cells were significantly higher in all Rhodamine B groups compared to control group (P < 0.05). The MDA levels were increased in a dose-dependent manner in the Rhodamine B groups. Moreover, the proliferation of epithelial cells was also increased by Rhodamine B in a dose-dependent manner. In conclusion, subchronic Rhodamine B administration induces lipid peroxidation and cervical epithelial cells proliferation in a dose dependent manner.

ABSTRAKTingkat keberhasilan pemberian Air Susu Ibu pada dua bulan pertama masih rendah, padahal masa ini merupakan masa percepatan pertumbuhan pada bayi, disaat kebutuhan bayi meningkat. Penelitian ini bertujuan untuk menganalisis pengaruh faktor sosial ibu meliputi umur, pendidikan, pekerjaan, pengetahuan, Inisiasi Menyusu Dini (IMD), dukungan suami dan teknik menyusui terhadap keberhasilan menyusui pada dua bulan pertama di RSKDIA Pertiwi Makassar. Metode penelitian ini adalah observasional analitik, dengan purposive sampling sebanyak 131 di Rumah Sakit Khusus Daerah Ibu dan Anak Pertiwi Makassar pada Oktober 2013-Januari 2014. Pengukuran teknik menyusui digunakan lembar observasi/check list yang dinilai oleh peneliti sendiri, sedangkan penilaian keberhasilan menyusui dilakukan melalui kunjungan rumah setelah subjek pulang dari Rumah Sakit, yakni pada satu minggu, dua minggu dan delapan minggu setelah subjek melahirkan, dengan menggunakan kuesioner. Analisis hasil penelitian menggunakan uji Chi-Square. Hasil penelitian menemukan ada pengaruh positif antara pendidikan (p=0,006; OR=2,826), pekerjaan (p=0,001; OR=0,293), pengetahuan (p=0,000; OR=14,792), IMD (p=0,000; OR=6,771), dukungan suami (p=0,000; OR=10,988) dan teknik menyusui (p=0,001; OR=3,784) terhadap keberhasilan menyusui pada dua bulan pertama. Hasil penelitian ini menyimpukan bahwa ibu dengan tingkat pendidikan tinggi, tidak bekerja, mempunyai pengetahuan yang baik, melaksanakan IMD, mempunyai dukungan aktif dari suami, memiliki teknik menyusui yang baik dapat meningkatkan keberhasilan menyusui pada dua bulan pertama.Kata Kunci: Air Susu Ibu, faktor sosial ibu, keberhasilan menyusui dua bulan pertama ABSTRACTThe success rate of breastfeeding in the first two months is still low, whereas this stage is a stage of accelerated growth in babies. This study aims to analyze the influence of maternal social factors including age, education, occupation, knowledge, early initiation of breastfeeding, husband's support, and feeding techniques to successfully breastfeed in the first two months in RSKDIA Pertiwi Makassar. The research method was an observational analytic study, with purposive sampling as many as 131 in Pertiwi Maternal and Child Special Hospital Makassar from October 2013 to January 2014. Breastfeeding technique measurement was using observation sheet/check list assessed by the researchers themselves, while the successful breastfeeding assessment was using questionnaire through home visits after the subjects were home, which were at one week, two weeks and eight weeks after subjects gave birth. Analyses of the research results were using Chi-Square. The study finds positive relationship between education (The results of this study concludes that mothers who have higher levels of education, do not work, have broad knowledge, implement the early initiation of breastfeeding, are supported actively by the husband, and have a good breastfeeding technique can improve the success of breastfeeding in the first two months.

Objectives Preeclampsia affects 3%–8% of all pregnancies. Thymoquinone is the primary compound in black cumin ( Nigella sativa ) and may have potential therapeutic effects in preeclampsia. This research analyses the effects of a black cumin seed ethanol extract on angiotensin II type 1-receptor autoantibody (AT1-AA) serum levels and the expression of the endothelin-1 (ET-1) in the placenta in preeclampsia mouse model. Methods The research design utilizes a post-test only experimental model on a control group design with 6 mice groups (negative control; positive control; and 500, 1000, 1500, and 2000 mg/kg body weight/day). Result The results showed a decrease in serum AT1-AA levels and ET-1 expression in the placenta by increased doses of black cumin with an optimal dose of 1000 mg/kg/day. Conclusions Black cumin seed ethanol extract reduces AT1-AA serum levels and represses ET-1 expression in the placenta in a preeclampsia mouse model.

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Inisiasi Menyusu Dini (IMD) adalah proses alami dimana bayi mulai menyusu sendiri segera setelah lahir kontak kulit dengan kulit ibunya hingga 1 (satu) jam setelah lahir. Meskipun IMD wajib dilakukan bidan dalam proses persalinan namun belum semua bidan melaksanakannya dengan baik. Terdapat beberapa faktor intrinsik dan ekstrinsik yang mempengaruhi pelaksanaan IMD. Penelitian ini bertujuan mengetahui pengaruh faktor intrinsik yang meliputi karakteristik bidan (umur, pendidikan, masa kerja), pengetahuan, sikap dan motivasi dan faktor ekstrinsik bidan yang meliputi pelatihan, beban kerja dan imbalan terhadap pelaksanaan Inisiasi Menyusu Dini di Puskesmas Rawat Inap Kabupaten Pasuruan. Penelitian ini menggunakan desain penelitian observasional analitik secara case control dan dengan tehnik purposive sampling. Penelitian ini dilaksanakan di 3 (tiga) Puskesmas Rawat Inap Kabupaten Pasuruan yaitu Puskesmas Gondangwetan, Purwodadi dan Winongan pada bulan November-Desember 2013. Besar sampel dalam penelitian ini adalah 40 orang. Setiap subjek diobservasi dengan lembar observasi saat melaksanakan IMD kemudian mengisi kuisioner untuk mengukur faktor intrinsik yaitu karakteristik bidan (umur, pendidikan, masa kerja), pengetahuan, sikap dan motivasi dan faktor ekstrinsik bidan yaitu pelatihan, beban kerja dan imbalan. Analisis hasil penelitian menggunakan uji analisis jalur. Hasil penelitian menemukan bahwa faktor intrinsik dan faktor ekstrinsik bidan berpengaruh terhadap pelaksanaan IMD di Puskesmas Rawat Inap Kabupaten Pasuruan. Variabel yang berpengaruh langsung terhadap pelaksanaan IMD di Puskesmas Rawat Inap Kabupaten Pasuruan adalah sikap bidan (2,78%), beban kerja yaitu jumlah persalinan/minggu (0,26%) dan jumlah jam kerja bidan/minggu (1,54%) serta motivasi (0,44%). Umur (14,51%), pendidikan (2%), masa kerja (7,67%), pengetahuan (0,26%), pelatihan (8%), dan imbalan (0,06%) merupakan faktor yang berpengaruh tidak langsung terhadap pelaksanaan IMD di Puskesmas Rawat Inap Kabupaten Pasuruan. Umur merupakan faktor yang paling berpengaruh terhadap pelaksanaan IMD di Puskesmas Kabupaten Pasuruan.

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Breastfeeding has been normative prevailing standard for feeding and fulfilling nutrition for baby due to its important benefit for mothers and her infant. The most common problem in breastfeeding is lack supply of breast milk after giving birth. The aim of this research was to prove that combination of acupressure and affirmation relaxation method was able to increase breast milk and improve breastfeeding self-efficacy of primiparous mothers in the postpartum period. The study design was true experimental with pre-test and post-test design. There were 24 primiparous mothers who were divided into two groups. Weighing test was used to measure breast milk production and breastfeeding self-efficacy was measured using Indonesian version of Breastfeeding Self-Efficacy Scale. Data were analyzed using Unpaired t-test and Mann Whitney test (α = 0.05). Based on the finding, we could conclude that combination of acupressure and affirmation relaxations could improve breast milk production (p = 0.033) and breastfeeding self-efficacy (p = 0.045) for primiparous mother in the postpartum period encountered in several Independent Midwifery Practice in Malang. This method might be used as a nonpharmacological treatment for postpartum mothers. Health practitioners should disseminate this technique so that postpartum mothers can do that by themselves.

Cervical cancer is the fourth most common cause of cancer in the world in women. The high mortality rate is due to the low awareness of women doing early detection. Behavior can be influenced by threat assessment, one of which is perceived severity. The behavior of early detection of cervical cancer can arise due to the perception of the severity of cervical cancer. Women's concern and desire for early detection of cervical cancer is influenced by women's perceptions of cancer. This study aims to explore the perception of the severity of women at high risk of cervical cancer regarding cervical cancer screening using the IVA method. This type of research is a qualitative research with a descriptive phenomenological approach. The research sample used purposive sampling technique. The study was conducted from April to May 2022 in the working area of ​​the Ujung Batu Health Center, 10 participants were interviewed for 25-35 minutes. The variable in this study is the perception of the severity of cervical cancer screening using the IVA method. The instrument used in this study is an interview guide and analyzed by thematic analysis. The results showed that all participants knew that the severity of cervical cancer can harm women and can cause death. The conclusion is that the awareness of participants in cervical cancer screening is still lacking. All participants understand the severity and seriousness of cervical cancer but have not screened for cervical cancer yet.