Protective effect of combined vitamin C and E against ovarian and endometrial toxicity in rats that receiving oral rhodamine B

Laili, Anis Nur; Ananingsih, Intin; Wiyasa, I Wayan Arsana; Indrawan, I Wayan Agung; Barlıanto, Wisnu; Yueniwati, Yuyun

doi:10.1016/j.bgm.2015.11.001

Cited by 7 publications

(4 citation statements)

References 20 publications

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“…Normal uterine epithelial structure is important for implantation of fertilized oocytes and therefore may play a vital role in fertility. This finding agrees with earlier work that vitamin C protect the uterus via protecting endometrial toxicity (Laili et al 2015).…”

Section: Discussionsupporting

confidence: 93%

Vitamin C reduced Pausinytalia Yohimbe induced reproductive toxicity in female Wister rats

2022

JMPB

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Medicinal plants have been used as a source of ailments healing for several years and had gained acceptance as well as widespread use in many cultures of the world. Pausinytalia Yohimbe (P.yohimbe) had been used in folklore medicine by tribes in Nigeria as aphrodisiac. Despite its beneficial effects as an aphrodisiac, we had demonstrated that chronic use of P.yohimbe had adverse effects on both male and female reproductive systems as evidenced by its effects on hormonal and structural changes in the reproductive systems. This study was done to determine if Vitamin C a potent anti oxidant can ameliorate the toxic effects of P.yohimbe.Twenty female Wistar rats weighing 160 -200g with normal estrus cycles were randomly allotted into four experimental groups of five rats each. Group A was the control group while groups B, C and D served as test groups. Group A received distilled water while group B received 5% Vitamin C only, group C received 150mg/kg body weight and D received vitamin C and 150mg/kg body weight of aqueous extract of P. yohimbe respectively for 4 weeks via oral gavage. Estrus cycles of the rats were evaluated daily and were sacrificed after 4 weeks. Blood samples obtained were used for hormonal assay (follicle stimulating hormone (FSH) and Luteinizing hormone (LH), estradiol and progesterone). Ovaries and uteri removed were used for Heamatoxylin and Eosin staining for histology.Vitamin C restored normal estrus cycle, improved gonadotropins (FSH and LH) production, and also ameliorated the degenerative changes in both ovaries and uteri of rats treated with P. yohimbe. Vitamin C had no significant effects on estradiol and progesterone production of rats treated with P. yohimbe.We concluded that Vitamin C ameliorated P. yohimbe induced reproductive toxicity in female Wistar rats. It may be that the mechanism of P. yohimbe induced reproductive tissue destruction and gonadotropins decrease is via oxidative stress. Vitamin C may be recommended for those chronically exposed to P. yohimbe for prevention.

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Section: Discussionsupporting

confidence: 93%

Vitamin C reduced Pausinytalia Yohimbe induced reproductive toxicity in female Wister rats

2022

JMPB

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“…e role of OS in the pathophysiology of endometriosis is well demonstrated in studies suggesting that the reduction OS is a treatment option [24]. Vitamin C and vitamin E can have interactive effects to reduce cellular damage induced by ROS [25]. It seems vitamin C and vitamin E can be used to neutralize the oxidative damage in endometriosis.…”

Section: Discussionmentioning

confidence: 99%

The Effect of Combined Vitamin C and Vitamin E Supplementation on Oxidative Stress Markers in Women with Endometriosis: A Randomized, Triple-Blind Placebo-Controlled Clinical Trial

Amini

Chekini

Nateghi

et al. 2021

Pain Research and Management

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Background. Endometriosis is a chronic and estrogen-dependent pelvic inflammatory disease, which may have various causes, such as oxidative stress. Dysmenorrhea, dyspareunia, and pelvic pain are well-known symptoms of endometriosis. The present clinical trial assessed the role of supplementation with antioxidant vitamins on the indices of oxidative stress as well as the severity of pain in women with endometriosis. Materials and Methods. We enrolled 60 reproductive-aged (15–45 years) women with pelvic pain in this triple-blind clinical trial. They had 1–3 stages of laparoscopic-proven endometriosis. The participants were randomized to group A (n = 30), given vitamin C (1000 mg/day, 2 tablets of 500 mg each) and vitamin E (800 IU/day, 2 tablets of 400 IU each) combination, or group B (n = 30), given placebo pills daily for 8 weeks. Results. Following treatment with vitamin C and vitamin E, we found a significant reduction in MDA and ROS compared with the placebo group. There was no significant decline in total antioxidant capacity after treatment. However, the severity of pelvic pain ( p value <0.001), dysmenorrhea ( p value <0.001), and dyspareunia ( p value <0.001) significantly decreased in the treatment group after 8 weeks of supplementation. Conclusions. The present findings support the potential role of antioxidants in the management of endometriosis. The intake of vitamin C and vitamin E supplements effectively reduced dysmenorrhea severity and improved dyspareunia and severity of pelvic pain.

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“…For mPEO- b -PPS-rhoda NPs, the viability was slightly affected by the presence of the rhodamine-B in RAW cells lines independent of NP sizes or concentration; however, the viability values are still considered satisfactory and were compared with values observed for the FDA-approved polymer polylactic acid (PLA) . It is important to highlight that rhodamine B is considered toxic to cell lines − at higher concentrations (Figure S9, RhodB); however, viability data values around 70% are considered normal, especially at the higher concentrations of this study (800 μg·mL –1 ). Taking into account the uptake studies, the NPs concentration utilized were 8 times lower (∼100 μg·mL –1 ).…”

Section: Results and Discussionmentioning

confidence: 86%

Poly(ethylene oxide monomethyl ether)-block-poly(propylene succinate) Nanoparticles: Synthesis and Characterization, Enzymatic and Cellular Degradation, Micellar Solubilization of Paclitaxel, and in Vitro and in Vivo Evaluation

Jäger

Syrová³

et al. 2018

Biomacromolecules

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Polyester-based nanostructures are widely studied as drug-delivery systems due to their biocompatibility and biodegradability. They are already used in the clinic. In this work, we describe a new and simple biodegradable and biocompatible system as the Food and Drug Administration approved polyesters (poly-ε-caprolactone, polylactic acid, and poly(lactic- co-glycolic acid)) for the delivery of the anticancer drug paclitaxel (PTX) as a model drug. A hydrophobic polyester, poly(propylene succinate) (PPS), was prepared from a nontoxic alcohol (propylene glycol) and monomer from the Krebs's cycle (succinic acid) in two steps via esterification and melt polycondensation. Furthermore, their amphiphilic block copolyester, poly(ethylene oxide monomethyl ether)- block-poly(propylene succinate) (mPEO- b-PPS), was prepared by three steps via esterification followed by melt polycondensation and the addition of mPEO to the PPS macromolecules. Analysis of the in vitro cellular behavior of the prepared nanoparticle carriers (NPs) (enzymatic degradation, uptake, localization, and fluorescence resonance energy-transfer pair degradation studies) was performed by fluorescence studies. PTX was loaded to the NPs of variable sizes (30, 70, and 150 nm), and their in vitro release was evaluated in different cell models and compared with commercial PTX formulations. The mPEO- b-PPS copolymer analysis displays glass transition temperature < body temperature < melting temperature, lower toxicity (including the toxicity of their degradation products), drug solubilization efficacy, stability against spontaneous hydrolysis during transport in bloodstream, and simultaneous enzymatic degradability after uptake into the cells. The detailed cytotoxicity in vitro and in vivo tumor efficacy studies have shown the superior efficacy of the NPs compared with PTX and PTX commercial formulations.

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Protective effect of combined vitamin C and E against ovarian and endometrial toxicity in rats that receiving oral rhodamine B

Cited by 7 publications

References 20 publications

Vitamin C reduced Pausinytalia Yohimbe induced reproductive toxicity in female Wister rats

Vitamin C reduced Pausinytalia Yohimbe induced reproductive toxicity in female Wister rats

The Effect of Combined Vitamin C and Vitamin E Supplementation on Oxidative Stress Markers in Women with Endometriosis: A Randomized, Triple-Blind Placebo-Controlled Clinical Trial

Poly(ethylene oxide monomethyl ether)-block-poly(propylene succinate) Nanoparticles: Synthesis and Characterization, Enzymatic and Cellular Degradation, Micellar Solubilization of Paclitaxel, and in Vitro and in Vivo Evaluation

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