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The aim of this experiment was to determine the effects of dietary supplementation of Bio‐Mos® as a source of mannan oligosaccharide (MOS) on the growth, survival, immunity and digestive enzyme activity of freshwater crayfish, Cherax destructor (yabby). A basal diet and another diet with 0.4% of Bio‐Mos® supplemented to the basal diet were formulated. Each diet was provided to eight yabbies (35.14 ± 0.48 g, initial weight) replicated 3 times in individual 250‐L plastic cylindrical tanks for a duration of 56 days. Each tank was provided with an independent recirculating water system filtered with a biological filtration. Growth parameters such as weight, specific growth rate and average weekly gain of Bio‐Mos®‐fed yabbies were higher (P < 0.05) than yabbies fed the control diet. Total haemocyte count, granular cells and semi‐granular cells were also higher in the yabbies fed Bio‐Mos® diet. Protease activity was higher in hepatopancreas, and amylase activity was higher in the guts of yabbies fed Bio‐Mos® diet. The results implies that supplementation of MOS through Bio‐Mos® can effectively improve growth and immunological condition of yabbies.

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The aim of this experiment was to determine the effects of dietary inclusion with mannan oligosaccharide (Bio-Mos, Alltech, Nicholasville, KY, USA) on growth, survival, physiological and immunological conditions and gut morphology of the black tiger prawn (Penaeus monodon). Five diets supplemented with MOS at 0 g kg À1 (control diet), 1, 2, 4 and 8 g kg À1 were fed to the prawn juveniles (0.4 AE 0.06 g, total weight) for the duration of 63 days. Growth was the highest (P < 0.05) when the prawns were fed the 1 g kg À1 MOS included diet. Wet tail muscle index (Tw/B), dry tail muscle index (Td/B) and tail muscle protein (Tmp) were higher (P < 0.05) in the prawns fed MOS included diets MOS compared with the prawns fed the control diet. Total haemocyte counts (THCs) of the prawns fed MOS included diets were higher (P < 0.05) than THCs of the prawns fed the control diet. Epithelium layer and epidermal cell density of the gut of the prawns fed 1 g kg À1 and 2 g kg À1 MOS diets were better than the prawns fed the control and other MOS diets. The results imply a positive effect of dietary supplementation of 1-2 g kg À1 MOS in the culture of black tiger prawns.

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The aim of this experiment was to determine the effects of dietary supplementation with mannan oligosaccharide (MOS; BioMos, Alltech, USA) on the gut microbiota and histology of marron, Cherax tenuimanus. Marron were fed six different diets supplemented with 0%, 0.05%, 0.1%, 0.2%, 0.4%, and 0.8% of MOS for a duration of 112 days. Total bacteria in the gut were highest when marron were fed diets which included 0.1% to 0.4% BioMos, whereas total bacteria/Vibrio spp. ratio was highest in the marron fed 0.4% BioMos diet only. The marron fed 0.2% and 0.4% BioMos diets showed normal morphology and higher densities of microvilli, and thicker layer of gut epithelium than other inclusion rates. The results suggest that application of 0.2% to 0.4% of MOS in the diet improves digestive system health in marron.