Dietary β-glucan improved growth performance, Vibrio counts, haematological parameters and stress resistance of pompano fish, Trachinotus ovatus Linnaeus, 1758

Huu, Hoang Do; Sang, Huynh Minh; Thủy, Nguyễn Thị Thanh

doi:10.1016/j.fsi.2016.03.161

Cited by 77 publications

(48 citation statements)

References 55 publications

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“…β‐glucan concentration correlates negatively with the quantity of Vibrio sp. in pompano fish Trachinotus ovatus (L. 1758) (Do Huu et al ., ) and for tinfoil barb Barbonymus schwanenfeldii (Bleeker 1854) inclusion of β‐glucan in the diet at 1% resulted in reduced bacterial diversity in the gut compared with those fed at 0.1% (Jung‐Schroers et al ., ). In contrast, β‐glucan did not impact species diversity of either allochthonous or autochthonous microbiota populations in the gut of Nile tilapia (Ran et al ., ).…”

Section: Introductionmentioning

confidence: 99%

Effect of β‐1/3,1/6‐glucan upon immune responses and bacteria in the gut of healthy common carp (Cyprinus carpio)

Harris

Bray

Adamek

et al. 2019

Journal of Fish Biology

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β-glucans are frequently included in the diet of healthy common carp Cyprinus carpio as a pre-emptive measure for combatting disease. In order to study the effect this has on the relationship between the gut bacteria and host immune response, carp were maintained on either a β-glucan free diet or feed containing 0.1% MacroGard ® , a β-1/3, 1/6-glucan, for up to 7 weeks and analysis of innate immune gene expression and molecular analysis of the gut bacteria was performed. The data reveals feeding of MacroGard ® to healthy carp does not induce bactericidal innate immune gene expression in the gut but does appear to alter bacterial species richness that did not have a negative effect on overall health. Analysis of innate immune gene expression within the upper midgut revealed that there were significant changes over time in the expression of Interleukin (il)-1β, inducible nitric oxide synthase (inos), mucin (muc2) and C-reactive protein (crp2). Diet did not affect the number of copies of the bacterial 16s rDNA gene in the gut, used as a as a measure of total bacteria population size. However, PCR-denaturing gradient gel electrophoresis (DGGE) analysis revealed a shift in bacterial species richness with MacroGard feeding. Bactericidal immune gene expression of crp2, muc2 and il-1β was weakly correlated with gut bacteria population size indicating a potentially limited role of these genes in interacting with the gut bacteria in healthy carp in order to maintain gut homeostatic conditions. These findings highlight the importance of considering both host immunity and the microbiome together in order to fully elucidate the effeect of immunomodulants, such as β-glucans, upon gut health.

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Section: Introductionmentioning

confidence: 99%

Effect of β‐1/3,1/6‐glucan upon immune responses and bacteria in the gut of healthy common carp (Cyprinus carpio)

Harris

Bray

Adamek

et al. 2019

Journal of Fish Biology

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“…Antibiotics and chemotherapeutics are effective to control diseases for long time; however, they could bring about side effects such as drug resistance to various pathogens, environmental pollution and health hazard to human (Meena et al, ). Immunostimulant is considered as one of the substitutes for antibiotics because it has no negative effects in fish (Do Huu, Sang, & Thanh Thuy, ). β‐glucan can enhance immunity and control diseases in different fishes, such as rainbow trout (Ghaedi, Keyvanshokooh, Mohammadi Azarm, & Akhlaghi, ), common carp (Falco et al, ) and Atlantic salmon (Refstie, Baeverfjord, Seim, & Elvebø, ; Rodríguez, Valenzuela, Farías, Sandino, & Imarai, ).…”

Section: Introductionmentioning

confidence: 99%

Dietary β‐glucan modulate haematological parameters, cytokines and gene expression in TLR and ERK pathways of rainbow trout ( Oncorhynchus mykiss ) during infection by Aeromonas salmonicida

Sun

et al. 2019

Aquac Res

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To explore the role of β‐glucan (0, 0.05%, 0.1% and 0.2%) in resisting bacteria, rainbow trout (Oncorhynchus mykiss) was challenged with Aeromonas salmonicida after 42‐day β‐glucan administration, and then sampled at 0, 2, 4 and 6 days post infection (dpi). The data showed that 0.2% β‐glucan reduced the accumulated mortality rates compared with the ICG (infected control group) (p < .05). The white blood cells, red blood cells and haemoglobin were higher in the 0.2% β‐glucan group (BG) than the ICG (p < .05). 0.1% and 0.2% β‐glucan elevated serum total antioxidative capability and glutathione activity but alleviated the increase of serum alkaline phosphatase activity and glucose concentration (p < .05) during infection. Serum TNF‐α, IL‐1β, IL‐8 and IgM in three BGs elevated remarkably on 6 dpi compared with the ICG (p < .05). Expression of tnf, il1b and cxcl8 of the head kidney in the 0.1% and 0.2% BGs was higher than the ICG on 4 dpi while ighm expression in the 0.2% BG was higher than in the ICG on 2 and 6 dpi (p < .05). 0.1% and 0.2% β‐glucan increased the expression of tlr5m, tlr5s, tmek and myd88 in the spleen after infection (p < .05). Similarly, 0.2% β‐glucan up‐regulated the expression of tmek, myd88, oncmyk‐dab and c3 in head kidney (p < .05). Overall, 0.2% dietary β‐glucan effectively decreased accumulated mortality rate by modulating the biochemistry process, cytokines, and activating TLR and ERK signalling pathways during A. salmonicida infection.

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“…There seems to be a dose-dependent effect when b-glucans are orally administered with the feed. The optimal level for pampona fish (Trachinotus ovatus) and striped catfish (Pangasianodon hypothalamus) seems to lie between 0.5 and 1 g/kg of feed (Soltanian, Adloo, Hafeziyeh, & Ghadimi, 2014;Huu et al, 2016). In the present study, common carp were fed with a diet supplemented with 1% Macro-Gard â , which might be a proper concentration for positive effects of b-glucans.…”

Section: Discussionmentioning

confidence: 90%

“…in the b-glucan fed carp. Reduced intestinal Vibrio counts were also seen in pompano fish (Trachinotus ovatus) when fed any level (0.5, 1, 2 or 4 g/kg) of b-glucan (Huu, Sang, & Thuy, 2016;).…”

Section: Discussionmentioning

confidence: 94%

Response of the intestinal mucosal barrier of carp (Cyprinus carpio) to a bacterial challenge by Aeromonas hydrophila intubation after feeding with β‐1,3/1,6‐glucan

Jung‐Schroers¹,

Adamek²,

Harris

et al. 2018

Journal of Fish Diseases

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The effect of dietary β-glucan on the bacterial community in the gut of common carp (Cyprinus carpio) was examined after oral application of Aeromonas hydrophila. Carp received either feed supplemented with 1% MacroGard , a β-1,3/1,6-glucan, or a β-glucan-free diet. Fourteen days after feeding, half of the carp from each group were intubated with 10 colony-forming units (CFU) of a pathogenic strain of A. hydrophila. Gut samples were taken 12 hr to 7 days after application and analysed using microbiological and molecular biological techniques (NGS, RT-PCR-DGGE). The reaction of the mucosa and the microbiota to an A. hydrophila intubation differed in carp fed with β-glucan compared to carp from the control group. In β-glucan fed carp, the total bacterial amount was lower but the number of bacterial species was higher. Bacterial composition was different for carp from both treatment groups. The number of mucin filled goblet cells was reduced in carp fed the β-glucan diet. Mucus was obviously released from the goblet cells and was probably washed out of the gut together with high numbers of bacteria. This might be protective against pathogenic bacteria and, therefore, feeding with β-glucan may provide protection against infections of the gut in carp.

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Dietary β-glucan improved growth performance, Vibrio counts, haematological parameters and stress resistance of pompano fish, Trachinotus ovatus Linnaeus, 1758

Cited by 77 publications

References 55 publications

Effect of β‐1/3,1/6‐glucan upon immune responses and bacteria in the gut of healthy common carp (Cyprinus carpio)

Effect of β‐1/3,1/6‐glucan upon immune responses and bacteria in the gut of healthy common carp (Cyprinus carpio)

Dietary β‐glucan modulate haematological parameters, cytokines and gene expression in TLR and ERK pathways of rainbow trout ( Oncorhynchus mykiss ) during infection by Aeromonas salmonicida

Response of the intestinal mucosal barrier of carp (Cyprinus carpio) to a bacterial challenge by Aeromonas hydrophila intubation after feeding with β‐1,3/1,6‐glucan

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