Dietary supplementation of mannan oligosaccharide improves the immune responses and survival of marron, Cherax tenuimanus (Smith, 1912) when challenged with different stressors

Survival and Immunity of Marron Cherax cainii (Austin, 2002) Fed Bacillus mycoides Supplemented Diet under Simulated Transport

“…Marron from each treatment group were placed in a ventilated container prior to placing in the polystyrene boxes. Placing the marron in the ventilated container not only avoided the marron from mixing with different treatment groups, but also protected the marron from the moist foam layer and ice-gel bags, and was based on the method used in a previous study [6]. Subsequently, another moist foam layer and ice bag were placed over the marron ventilated containers, before the outer polystyrene box was sealed with a lid.…”

“…Practices and methods used for crustacean handling and live trade may lead to serious physiological stress responses in aquatic animals [1][2][3] including marron [4][5][6]. Marron, Cherax cainii (Austin, 2002), is endemic to Western Australia and recently was introduced into South Africa, Zimbabwe, Japan, USA, China and the Caribbean as a commercial aquaculture species [6][7][8].…”

“…Marron, Cherax cainii (Austin, 2002), is endemic to Western Australia and recently was introduced into South Africa, Zimbabwe, Japan, USA, China and the Caribbean as a commercial aquaculture species [6][7][8].…”

“…Marron are an economically important aquaculture species in Western Australia and they show significant environmental stress tolerance post handling and live transport. Jussila observed no mortality of marron post 24 h handling and simulated transport, whereas detected no mortality of marron up to 36 h under simulated transport [5,6]. Moreover, marron may undergo live transport up to 72 h without mortality, however longer periods of transportation resulted in an average dehydration of 4.5% of body weight [4,5].…”

“…Feed additives such as probiotics and prebiotics can improve stress tolerance and immunity of aquatic animals including marron [6,[11][12][13][14][15][16]. Our previous studies indicated that Bacillus mycoides, a marron origin customised probiotic, possessed a number of favourable probiotic properties [17], significantly improved gastrointestinal (GIT) health of marron [18] and improved immunity against the pathogenic bacterium Vibrio mimicus [19], a dominant bacterial pathogen of freshwater crayfish in aquaculture [20][21][22].…”