A Gram-negative, aerobic, non-motile, long rods or coccoid without flagellum strain, designated THG-YS3.2.7, was isolated from therhizosphere soil of a Mugunghwa flower collected from Kyung Hee University, Yongin, South Korea. Growth occurred at 10-40 °C (optimum 28-37 °C), at pH 6-8 (optimum 7) and at 0-5 % NaCl (optimum 1 %). The predominant ubiquinone was ubiquinone 8 (Q-8). The major cellular fatty acids were C10 : 0, C10 : 0 3OH, C16 : 0, C17 : 0, C17 : 0 cyclo, C18 : 0, C18 : 3ω6c (6,9,12), summed feature 3 (C16 : 1ω7c and/or C16 : 1ω6c) and summed feature 8 (C18 : 1 ω7c and/or C18 : 1ω6c). The major polar lipids were diphosphatidylglycerol (DPG), phosphatidylglycerol (PG), phosphatidylethanolamine (PE), phosphatidylcholine (PC). The DNA G+C content of strain THG-YS3.2.7 was 69.4 mol%. Based on 16S rRNA gene sequence analysis, the nearest phylogenetic neighbours of strain THG-YS3.2.7 were identified as Ramlibacter henchirensis DSM 14656 (97.92 %), Ramlibacter tataouinensis DSM 14655 (97.90 %), Ramlibacter solisilvae KACC 17567 (97.04 %). DNA-DNA hybridization values between strain THG-YS3.2.7 and R. henchirensis DSM 14656, R. tataouinensis DSM 14655, R. solisilvae KACC 17567 were 32.5±1.5, 43.1±1.1, 42.8±1.1 %, respectively. On the basis of the phylogenetic analysis, chemotaxonomic data, physiological characteristics and DNA-DNA hybridization data, strain THG-YS3.2.7 represent a novel species of the genus Ramlibacter, for which the name Ramlibacter rhizophilus sp. nov. is proposed. The type strain is THG-YS3.2.7 (=KCTC 52083=CCTCC AB 2015357).
A treatment using a mixture of herbs such as THAN-1 would be useful in the suppression of the growth of pathogenic bacterial and fungal strains.
A novel bacterial strain, designated THG-S11.7 T , was isolated from garden soil in Incheon, South Korea. Cells of the strain were Gram-stain-positive, aerobic, non-motile cocci, and were catalase-and oxidase-positive. Colonies of the strain were white. Strain THG-S11.7T grew optimally at 28 8C, at pH 7.0 and in the presence of 2.0 % NaCl. 16S rRNA gene sequence analysis indicated that the strain was a member of the genus Nocardioides. Strain THG-S11 T and the closest phylogenetic neighbours were below 45.0 % and the DNA G+C content of strain THG-S11.7 T was 72.2 mol%. Strain THG-S11.7 T was characterized chemotaxonomically as having LL-diaminopimelic acid in the cell-wall peptidoglycan and menaquinone MK-8(H 4 ) as the predominant isoprenoid quinone. The major phospholipid was determined to be diphosphatidylglycerol. The major cellular fatty acids of strain THG-S11.7 T were iso-C 15 : 0 , C 16 : 0 and iso-C 16 : 0 .Based on the phenotypic, genotypic and phylogenetic analyses, it is proposed that the isolate represents a novel species of the genus Nocardioides, for which the name Nocardioides albidus sp. nov. is proposed. The type strain is THG-S11.7 T (5KCTC 39607 T 5CCTCC AB 2015297 T ).
A Gram-stain-negative, aerobic, non-motile and coccus-shaped bacterium (THG-3.7 T) was isolated from seawater. Growth occurred at 10-30 C (optimum 25 C), at pH 6-8 (optimum 7) and in the presence of 1-8 % (w/v) NaCl (optimum 4 %). Based on 16S rRNA gene sequence analysis, the nearest phylogenetic neighbours of strain THG-3.7 T were identified as Paraglaciecola mesophila DSM 15026 T (95.3 % similarity), Glaciecola pallidula DSM 14239 T (95.2 %), Paraglaciecola aquimarina KCTC 32108 T (95.1 %), Paraglaciecola arctica KACC 14537 T (94.9 %), Glaciecola nitratireducens KCTC 12276 T (94.7 %) and Paraglaciecola psychrophila CGMCC 1.6130 T (94.7 %). 16S rRNA gene sequence similarities among strain THG-3.7 T and other species were lower than 94.7 %. The polar lipids were phosphatidylethanolamine, phosphatidylglycerol, diphosphatidylglycerol, one unidentified lipid and one unidentified aminolipid. The quinone system was composed of Q-8. The major fatty acids were C 16 : 0 , C 18 : 1 !7c and summed feature 3 (C 16 : 1 !7c and/or iso-C 15 : 0 2-OH). The DNA G+C content of strain THG-3.7 T was 47.9 mol%. On the basis of the data presented, strain THG-3.7 T represents a novel species of the genus Glaciecola, for which the name Glaciecola amylolytica sp. nov. is proposed. The type strain is THG-3.7 T (=KACC 19478 T =CCTCC AB 2017258 T).
scite is a Brooklyn-based organization that helps researchers better discover and understand research articles through Smart Citations–citations that display the context of the citation and describe whether the article provides supporting or contrasting evidence. scite is used by students and researchers from around the world and is funded in part by the National Science Foundation and the National Institute on Drug Abuse of the National Institutes of Health.
hi@scite.ai
10624 S. Eastern Ave., Ste. A-614
Henderson, NV 89052, USA
Copyright © 2024 scite LLC. All rights reserved.
Made with 💙 for researchers
Part of the Research Solutions Family.