Glaciecola amylolytica sp. nov., an amylase-producing bacterium isolated from seawater

Abstract

A Gram-stain-negative, aerobic, non-motile and coccus-shaped bacterium (THG-3.7 T) was isolated from seawater. Growth occurred at 10-30 C (optimum 25 C), at pH 6-8 (optimum 7) and in the presence of 1-8 % (w/v) NaCl (optimum 4 %). Based on 16S rRNA gene sequence analysis, the nearest phylogenetic neighbours of strain THG-3.7 T were identified as Paraglaciecola mesophila DSM 15026 T (95.3 % similarity), Glaciecola pallidula DSM 14239 T (95.2 %), Paraglaciecola aquimarina KCTC 32108 T (95.1 %), Paraglaciecola arctica KACC 14537 T (94.9 %), Glaciecola nitratireducens KCTC 12276 T (94.7 %) and Paraglaciecola psychrophila CGMCC 1.6130 T (94.7 %). 16S rRNA gene sequence similarities among strain THG-3.7 T and other species were lower than 94.7 %. The polar lipids were phosphatidylethanolamine, phosphatidylglycerol, diphosphatidylglycerol, one unidentified lipid and one unidentified aminolipid. The quinone system was composed of Q-8. The major fatty acids were C 16 : 0 , C 18 : 1 !7c and summed feature 3 (C 16 : 1 !7c and/or iso-C 15 : 0 2-OH). The DNA G+C content of strain THG-3.7 T was 47.9 mol%. On the basis of the data presented, strain THG-3.7 T represents a novel species of the genus Glaciecola, for which the name Glaciecola amylolytica sp. nov. is proposed. The type strain is THG-3.7 T (=KACC 19478 T =CCTCC AB 2017258 T).

A case study of biofilter activation and microbial nitrification in a marine recirculation aquaculture system for rearing Atlantic salmon (Salmo salarL.)

“…However, the differences in rearing conditions allowed us to compare 'cold start' with high stocking densities and low stocking densities. Bernardet, 2015;Bowman & McMeekin, 2015;Deming & Junge, 2015;Kim, 2015;McIlroy & Nielsen, 2014;Nedashkovskaya & Kim, 2019;Xiao et al, 2019). However, few of the dominating taxa detected at genus level in this study were recognized among dominating taxa described in previous studies from aquaculture systems (Brailo et al, 2019;Rurangwa & Verdegem, 2015;Schreier et al, 2010).…”

“…The analysed biofilms in the two biofilters had many taxa in common at the end of the rearing cycle. The dominating taxa common for both biofilters included Neptuniibacter , Glaciecola , Colwellia , Kordia , Flavobacterium , Algibacter , Planctomycetes (OM190) and members within Saprospiraceae and Alteromonadaceae , which were closely related to cultured organoheterotrophic species isolated from seawater and marine habitats worldwide (Arahal et al., 2007; Bernardet, 2015; Bowman & McMeekin, 2015; Deming & Junge, 2015; Kim, 2015; McIlroy & Nielsen, 2014; Nedashkovskaya & Kim, 2019; Xiao et al., 2019). However, few of the dominating taxa detected at genus level in this study were recognized among dominating taxa described in previous studies from aquaculture systems (Brailo et al., 2019; Rurangwa & Verdegem, 2015; Schreier et al., 2010).…”

Manipulation in grazing, viral pressure and resource availability leads to success in the isolation of abundant marine bacteria

“…In the DL and VL treatments (t f ), the most abundant uncultured taxa accounting for a high proportion of fall, winter and spring reads affiliated to a total of 12 species in the NCBI 16S rRNA database (Table S8) which had been isolated in different culture media and/or from other environments than the ones in this study (see references in Table S8). Only three of them had originally been isolated in MA from seawater: Donghicola eburneus (50), Glaciecola amylolytica (although it was isolated at 30°C; 51) and Altibacter lentus (isolated from 2000-m deep seawater; 52). It is also important to note that the cultures here were incubated at room temperature (RT; 20-25°C) and summer and the fall were the seasons with the closest in situ temperature to RT (23.1°C in summer, 19.5°C in fall; 37).…”

Activity-Based Screening of Soil Samples from Nyingchi, Tibet, for Amylase-Producing Bacteria and Other Multifunctional Enzyme Capacities

“…On the other hand, further screening of amylases for specifc adaptive characteristics (e.g., thermophilic, psychrophilic, acidophilic, and alkaliphilic amylases) in a unique natural environment should be focused mainly on [22,23]. To date, microbial amylases with unique properties isolated from water samples (deep sea, hot springs, and lakes) have been well documented [11,20,[24][25][26]. In contrast, soilderived amylase-producing bacteria with superior enzymatic characteristics have received relatively little attention.…”