Ramlibacter rhizophilus sp. nov., isolated from rhizosphere soil of national flower Mugunghwa from South Korea

Abstract

A Gram-negative, aerobic, non-motile, long rods or coccoid without flagellum strain, designated THG-YS3.2.7, was isolated from therhizosphere soil of a Mugunghwa flower collected from Kyung Hee University, Yongin, South Korea. Growth occurred at 10-40 °C (optimum 28-37 °C), at pH 6-8 (optimum 7) and at 0-5 % NaCl (optimum 1 %). The predominant ubiquinone was ubiquinone 8 (Q-8). The major cellular fatty acids were C10 : 0, C10 : 0 3OH, C16 : 0, C17 : 0, C17 : 0 cyclo, C18 : 0, C18 : 3ω6c (6,9,12), summed feature 3 (C16 : 1ω7c and/or C16 : 1ω6c) and summed feature 8 (C18 : 1 ω7c and/or C18 : 1ω6c). The major polar lipids were diphosphatidylglycerol (DPG), phosphatidylglycerol (PG), phosphatidylethanolamine (PE), phosphatidylcholine (PC). The DNA G+C content of strain THG-YS3.2.7 was 69.4 mol%. Based on 16S rRNA gene sequence analysis, the nearest phylogenetic neighbours of strain THG-YS3.2.7 were identified as Ramlibacter henchirensis DSM 14656 (97.92 %), Ramlibacter tataouinensis DSM 14655 (97.90 %), Ramlibacter solisilvae KACC 17567 (97.04 %). DNA-DNA hybridization values between strain THG-YS3.2.7 and R. henchirensis DSM 14656, R. tataouinensis DSM 14655, R. solisilvae KACC 17567 were 32.5±1.5, 43.1±1.1, 42.8±1.1 %, respectively. On the basis of the phylogenetic analysis, chemotaxonomic data, physiological characteristics and DNA-DNA hybridization data, strain THG-YS3.2.7 represent a novel species of the genus Ramlibacter, for which the name Ramlibacter rhizophilus sp. nov. is proposed. The type strain is THG-YS3.2.7 (=KCTC 52083=CCTCC AB 2015357).

Cassava/peanut intercropping improves soil quality via rhizospheric microbes increased available nitrogen contents

“…Ramlibacter tataouinensis TTB310 T (strain TTB310) is a beta-proteobacterium isolated from sand particles [55]. Different species from this genus were isolated in tropical forest soil [56] and the rhizospheric soil of Mugunghwa [57]. Furthermore, studies revealed that the genus of Ramlibacter has phosphatase activity [58] and ginsenoside-converting activity [59].…”

Ramlibacter pinisoli sp. nov., a novel bacterial species isolated from pine garden soil

“…At the time of writing, only seven species had been described, based on the List of Prokaryotic names with Standing in Nomenclature (LPSN): Ramlibacter henchirensis , Ramlibacter tataouinensis , Ramlibacter solisilvae , Ramlibacter rhizophilus , Ramlibacter ginsenosidimutans , Ramlibacter monticola and Ramlibacter alkalitolerans . All of these seven species were isolated from different soil samples collected in forest [2, 3], rhizosphere [4], ginseng [5, 6] and subdesert [1]. The common characteristics of all species of the genus Ramlibacter are Gram-strain-negative and rod- or coccoid-shaped cells.…”

“…They contain ubquinone 8 (Q-8) as the major respiratory quinone and C 16 : 0 , summed feature 3 (C 16 : 1 ω 7 c and/or C 16 : 1 ω 6 c ) and summed feature 8 (C 18 : 1 ω 7 c and/or C 18 : 1 ω 6 c ) as the major fatty acid component. The DNA G+C content of members of this genus ranges from 66.6 to 69.9 mol% [1, 2, 4]. In this study, we report on the isolation and characterization of a novel bacterial species of the genus Ramlibacter , represented by strain MAH-25 T , isolated from soil sampled in a pine garden.…”

“…Growth was tested using several bacterial media such as NA (Difco), tryptone soya agar (TSA; Oxoid), R2A agar (Difco), Luria-Bertani (LB) agar (Oxoid) and MacConkey agar (Oxoid) at 28 °C. Growth at different temperatures (4,10,15,20,25,28,30,35, 40 and 45 °C) and at various pH levels (pH 3.0-10.0, at intervals of 0.5 pH units) was assessed in nutrient broth after 7 days of incubation at 28 °C. pH-dependent growth was tested using two different buffers (final concentration, 100 mM): acetate buffer was used for pH 3.0-6.5 and phosphate buffer was used for pH 7.0-10.0.…”

“…The biochemical and physiological characteristics of strain MAH-25 T and the reference strains of the genus Ramlibacter Strains: 1, MAH-25 T ; 2, R. solisilvae KACC 17567 T ; 3, R. henchirensis KACC 11925 T ; 4, R. tataouinensis DSM 14655 T ; 5, R. rhizophilus KCTC 52083 T . All data were obtained in this study, except a, b and c that were taken from[1,2,4], respectively. All strains are aerobic and rods or coccoid shaped.…”