Context: Almond oil is used in traditional and complementary therapies for its numerous health benefits due to high unsaturated fatty acids content.Objectives: This study investigated the composition and in vitro anticancer activity of almond oil from Northern Cyprus and compared with almond oil from Turkey.Materials and methods: Almond oil from Northern Cyprus was obtained by supercritical CO2 extraction and analyzed by GC-MS. Almond oil of Turkey was provided from Turkish pharmacies. Different concentrations of almond oils were incubated for 24 and 48 h with Colo-320 and Colo-741 cells. Cell growth and cytotoxicity were measured by MTT assays. Anticancer and antiprolifetarive activities of almond oils were investigated by immunocytochemistry using antibodies directed against to BMP-2, β-catenin, Ki-67, LGR-5 and Jagged 1.Results: Oleic acid (77.8%; 75.3%), linoleic acid (13.5%; 15.8%), palmitic acid (7.4%; 6.3%), were determined as the major compounds of almond oil from Northern Cyprus and Turkey, respectively. In the MTT assay, both almond oils were found to be active against Colo-320 and Colo-741 cells with 1:1 dilution for both 24 h and 48 h. As a result of immunohistochemical staining, while both almond oils exhibited significant antiproliferative and anticancer activity, these activities were more similar in Colo-320 cells which were treated with Northern Cyprus almond oil.Discussion and conclusion: Almond oil from Northern Cyprus and Turkey may have anticancer and antiproliferative effects on colon cancer cells through molecular signalling pathways and, thus, they could be potential novel therapeutic agents.
Background:
Quercetin is a flavonol from the flavonoid group of polyphenols which positively affects human health due to its
anti-cancer, anti-inflammatory, anti-microbial and cardioprotective effects. The effects of phenolic compounds,
including quercetin, on programmed cell death and cellular senescence have been the subject of research in
recent years.
Objective:
In this study, we aimed to investigate the effects of quercetin on cell viability, apoptosis and cellular senescence
in primary (Colo-320) and metastatic (Colo-741) colon adenocarcinoma cell lines.
Methods:
Cytotoxicity was analyzed via MTT assay in Colo-320 and Colo-741 cell lines. After quercetin treatment,
cellular senescence and apoptosis were evaluated by TUNEL staining, X-Gal staining and indirect peroxidase
technique for immunocytochemical analysis of related proteins such as Bax, Bcl-2, caspase-3, Hsp27, Lamin B1,
p16, cyclin B1.
Results:
The effective dose for inhibition of cell growth in both cell lines was determined to be 25µg/ml quercetin for 48
hours. Increased Bax immunoreactivity following quercetin treatment was significant in both Colo-320 and
Colo-741 cell lines, but decreased Bcl-2 immunoreactivity was significant only in the Colo-320 primary cell line.
In addition, after quercetin administration, the number of TUNEL positive cells and, immunoreactivities for p16,
Lamin B1 and cyclin B1 in both Colo-320 and Colo-741 cells increased.
Conclusion:
Our results suggest that quercetin may only induce apoptosis in primary colon cancer cells. Furthermore,
quercetin also triggered senescence in colon cancer cells but some cells remained alive, suggesting that colon
cancer cells might have escaped from senescence.
Introduction:
Diabetic burn wounds and ulcers are significant complications of diabetic patients. The aim of this study is to investigate the use of platelet rich-plasma (PRP) and/or keratinocyte-like cells (KLCs) in diabetic thermal wound rat model and to evaluate EGF, FGF-2, TGF-β1, COL1α2, MCP-1 and VEGF-α as wound healing markers at gene expression level.
Method:
In this study, we used adipose tissue as the source of mesenchymal stem cells (MSCs) and differentiated MSCs into KLCs. KLCs were characterized and transferred to the burn areas on the dorsum of streptozotocine (STZ)-induced diabetic rats. We prepared PRP from rat blood and evaluated its effect alone or in combination with KLCs. On 3rd, 7th, 10th and 14th days after treatment, wound areas were measured and biopsy samples were excised from the wound areas of the KLCs and/or PRP-treated and untreated diabetic rats to analyze gene expression levels of wound healing markers by qPCR.
Results:
We observed that, wound contraction started earlier in the PRP and/or KLCs-treated groups in comparison to the control group. However, PRP and KLCs when applied in combination showed additive affect in wound healing. In all groups treated with KLCs and/or PRP, the gene expression levels of evaluated growth factors and COL1α2 increased, while MCP-1 levels decreased when compared to the untreated diabetic rats. In addition, the most prominent difference in qPCR results belongs to combined PRP and KLCs-treated group.
Conclusion:
We demonstrated that applying PRP and KLCs in combination has a greater potential for treatment of diabetic burn wounds.
Opuntia ficus indica L. fruit (cactus pear) seed oil is used in traditional and complementary therapies for its numerous health benefits. The aim of this study was to analyse of the fatty acid content and apoptotic induction effects of spiny and thornless Opuntia ficus indica L. seed (CPS) oils. Spiny and thornless Opuntia ficus indica L. seed oils obtained by supercritical CO2 extraction method and analyzed by GC-MS. Different concentrations of almond oils were incubated for 24 h and 48 h with Colo-320 and Colo-741 cells. Cell growth and cytotoxicity were measured by MTT assays. TUNEL assay was used to detect DNA fragmentation in both cell lines. Linoleic acid was dominant fatty acid followed by oleic acid, palmitic acid and elaidic acid in both type of seed oil. In MTT, spiny CPS oil was found to be active against Colo-320 and Colo-741 cells with 1:16 dilution for 48 h. Also, 1:8 and 1:16 dilutions of thornless CPS oil showed significant reduction in the number of viable cells in Colo-320 and Colo-741 cells, respectively. The number of TUNEL positive cells were significantly higher in Colo-320 cells treated with thornless CPS when compare with control group (p < 0.05).We conclude that thornless CPS oil may have anticancer effect on primer colon adenocarcinoma cell lines. The effect can be explained by inducing apoptosis. Thus, they could be a potential novel therapeutic agent in colon cancer therapy.
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