Evaluation of low-level diode laser irradiation and various irrigant solutions on the biological response of stem cells from exfoliated deciduous teeth

Tunç, Hamit; İslam, Aylin; Kabadayı, Hilal; Vatansever, Hafize Seda; Yılmaz, Hasan Güney

doi:10.1016/j.jphotobiol.2019.01.001

Cited by 8 publications

(14 citation statements)

References 35 publications

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“…Table 1 summarizes the studies that evaluated growth factors’ release/expression (Aksel et al, 2020; Atesci et al, 2020; Bracks et al, 2019; Cameron et al, 2019; Chae et al, 2018; Deniz Sungur et al, 2019; Duncan et al, 2017; Galler et al, 2015; Gonçalves et al, 2016; Ivica et al, 2019; Kucukkaya Eren et al, 2021; Li et al, 2020; Liu et al, 2019; Ranc et al, 2018; Sadaghiani et al, 2016; Widbiller et al, 2017; Zeng et al, 2016). Table 2 summarizes information from the in vitro studies that evaluated cell behaviour (Aksel et al, 2020; Alghilan et al, 2017; Atesci et al, 2020; Chae et al, 2018; Deniz Sungur et al, 2019; Galler et al, 2016; Gonçalves et al, 2016; Hashimoto et al, 2018; Huang et al, 2011; Ivica et al, 2019; Kawamura et al, 2016; Kim et al, 2015; Kucukkaya Eren et al, 2021; Li et al, 2020; Liu et al, 2019; Martin et al, 2014; Mollashahi et al, 2016; Pang et al, 2014; Park et al, 2015; Prompreecha et al, 2018; Ring et al, 2008; Sadaghiani et al, 2016; Scott et al, 2018; Shrestha et al, 2016; Tunç et al, 2019; Widbiller et al, 2019), and Table 3 shows studies that evaluated tissue regeneration in immature teeth models (El Ashry et al, 2016; Kawamura et al, 2016; Taweewattanapaisan et al, 2019; Yamauchi et al, 2011). According to the study design, 32 studies were only in vitro , three studies were in vivo , and one study used both models.…”

Section: Resultsmentioning

confidence: 99%

Influence of ethylenediaminetetraacetic acid on regenerative endodontics: A systematic review

et al. 2022

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Background The effects of ethylenediaminetetraacetic acid (EDTA) on regenerative endodontic procedures (REPs) are controversial, because, despite releasing growth factors from dentine, some studies show negative effects on cell behaviour. Objectives The aim of the study was to investigate the influence of the use of EDTA in REP on the growth factors’ release, cell behaviour and tissue regeneration. Methods A systematic search was conducted (PubMed/Medline, Scopus, Cochrane Library, Web of Science, Embase, OpenGrey and reference lists) up to February 2021. Only in vivo and in vitro studies evaluating the effects of EDTA on the biological factors of dentine, pulp/periapical tissues and cell behaviour were eligible. Studies without a control group or available full text were excluded. The growth factors’ release was the primary outcome. Risk of bias in the in vitro and in vivo studies was performed according to Joanna Briggs Institute's Checklist and SYRCLE’s RoB tool, respectively. Results Of the 1848 articles retrieved, 36 were selected. Amongst these, 32 were in vitro, three animal studies and one with both models. The EDTA concentrations ranged from 3% to 15%, at different times. Regarding growth factors’ release (17 studies), 15 studies found significant transforming growth factor (TGF)‐β release after dentine conditioning with EDTA, and most found no influence on vascular endothelial growth factor release. Regarding cell behaviour (26 studies), eight studies showed no influence of EDTA‐treated dentine on cell viability, whereas, five, nine and six studies showed higher cell migration, adhesion and differentiation respectively. No influence of EDTA conditioning was observed in animal studies. In vitro studies had a low risk of bias, whereas animal studies had high risk of bias. Meta‐analysis was unfeasible. Discussion This review found that EDTA increased TGF‐β release and improved cell activity. However, well‐designed histological analyses using immature teeth models are needed. Conclusions High‐quality in vitro evidence suggests that EDTA‐treated dentine positively influences TGF‐β release, cell migration, attachment and differentiation; further research to evaluate its influence on tissue regeneration is necessary due to low methodological quality of the animal studies.

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Section: Resultsmentioning

confidence: 99%

Influence of ethylenediaminetetraacetic acid on regenerative endodontics: A systematic review

et al. 2022

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“…Although the antibacterial effect of ozone therapy has proven, cytotoxic or/and proliferative effect of ozonated water is rarely discussed [26,[31][32][33]. Therefore, the present study aimed to assess the biological response of dental primary pulp cells to various doses of ozonated water by using MTT assay.…”

Section: Discussionmentioning

confidence: 99%

“…In another study, cytotoxicity of OW with concentrations of 5 mg/L, 10 mg/L, and 20 mg/L were tested by using MTT assay at 5, 10 and 15-min time points of interaction with stem cells from human exfoliated deciduous teeth (hSHEDs). The study revealed that the 20 mg/L OW at 5 and 10-min time points showed the highest proliferation rate whereas the 5 mg/L ozonated water showed the highest proliferation rate at the 15-min time point [32]. The authors evaluated the cytotoxicity by using MTT assay and the cell viability results were given in percentages.…”

Section: Discussionmentioning

confidence: 99%

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Cytotoxicity assessment of different doses of ozonated water on dental pulp cells

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Yıldırım

Çetiner³

2021

BMC Oral Health

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Background The purpose of this study was to assess the cytotoxicity of various concentrations of ozonated water (OW) on human primary dental pulp cells. Methods Human primary dental pulp cells were isolated from exfoliated primary canine teeth of an 11-year-old patient with good systemic and oral health. Afterwards, cells were divided into 6 experimental groups; four groups of OW in concentrations of 2 mg/L, 4 mg/L, 8 mg/L, and 16 mg/L, untreated control group, and cell culture without cells. Cytotoxicity was evaluated after exposure for 5-min exposure using Mosmann’s Tetrazolium Toxicity (MTT) assay at 0 h and 48 h time points. Data were analyzed using a repeated measures analysis of variance and Post-hoc tests were performed using Bonferroni correction for multiple comparisons. Results All experimental groups showed proliferation at 0 h time point. However, all groups also experienced a decrease in overtime at 48 h time point (p < 0.05). At both time points 2 mg/L OW showed the highest cell viability as well as proliferation. At 0 h time point, the increase in cell viability for all experimental groups was found statistically significant when compared to positive control group (p < 0.05). At 48 h time point, although 8 mg/L and 16 mg/L OW showed statistically significant reduction in compare to 0 h time point, 2 mg/L and 4 mg/L OW groups didn’t experience any statistically significant difference (p < 0.05). Conclusion Considering our findings, due to ozonated water's induced a higher proliferation rate of dental pulp cells, indicating their biocompatibility and a possible adjuvant on irrigating agent in regenerative endodontic procedures.

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“…29 In addition to different cytotoxic properties, various mechanisms for the mitogenic effects of LPLT have also been suggested at specific intensities. 30,31 Therefore, it is reasonable to claim that the cell survival rate is dependently related to the irradiation intensity and the time period.…”

Section: Discussionmentioning

confidence: 99%

Low-Level Laser Irradiation Modulated Viability of Normal and Tumor Human Lymphocytes In Vitro

et al. 2020

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Introduction: Laser radiation is a promising strategy against various malignancies. Recent studies have shown that the application of low-power laser therapy (LPLT) at different doses and exposure times could modulate the growth dynamic of tumor cells. Based on the type of laser, LPLT could potentially trigger cell proliferation, differentiation, and apoptosis in different cell lines. Methods: In this study, MTT assay was used to monitor the effect of low and high laser intensities on the viability of normal and cancer lymphocytes. The protein levels of Ki-67 (a proliferation marker) and Caspase-3 (an apoptosis factor) were measured in human peripheral mononuclear cells (PBMCs) and the B-lymphoblastic cell line (Nalm-6) using flow cytometry after being-exposed to 630-nm LPLT at low (2, 4, 6, and 10 J/cm2 ) and high (15, 30, 60, and 120 J/cm2 ) energy densities in a continuous mode for 48 and 72 hours. Results: By using higher energy densities, 60 and 120 J/cm2 , a significant decrease was shown in the viability of Nalm-6 cells, which reached 6.6 and 10.1% after 48 hours compared to the control cells (P<0.05). Notably, Cell exposure to doses 30, 60, and 120 J/cm2 yielded 7.5, 12.9, and 21.6 cell viability reduction after 72 hours. The collected data showed that the high-intensity parameters of LPLT (15 to 120 J/cm2 ) promoted significant apoptotic changes in the exposed cells coincided with the activation of Caspase-3 compared to the none-treated control cells (P<0.05). The data further showed the stimulation of the Ki-67 factor both in primary PBMCs and the lymphoblastic cell line treated with LPLT at energy densities of 4 and 6 J/cm2 (P<0.05), indicating enhanced cell proliferation. Similar to Nalm-6 cells, primary PBMCs showed apoptosis after 48 hours of being exposed to doses 60, and 120 J/cm2 , indicated by increased Caspase-3 levels (P<0.05). As expected, the Nalm-6 cells were resistant to cytotoxic effects of laser irradiation in the first 48 hours (P>0.05) compared to normal PBMCs. The exposure of Nalm-6 cells to low-intensity laser intensities increased a proliferation rate compared to the PBMCs treated with the same doses. Conclusion: We showed the potency of LPLT in the induction of apoptosis and proliferation in human primary PBMCs and Nalm-6 cells in a dose and time-dependent manner after 72 hours.

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Evaluation of low-level diode laser irradiation and various irrigant solutions on the biological response of stem cells from exfoliated deciduous teeth

Cited by 8 publications

References 35 publications

Influence of ethylenediaminetetraacetic acid on regenerative endodontics: A systematic review

Influence of ethylenediaminetetraacetic acid on regenerative endodontics: A systematic review

Cytotoxicity assessment of different doses of ozonated water on dental pulp cells

Low-Level Laser Irradiation Modulated Viability of Normal and Tumor Human Lymphocytes In Vitro

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