Fatty acid composition and anticancer activity in colon carcinoma cell lines of
            <i>Prunus dulcis</i>
            seed oil

Meriçli, Filiz; Becer, Eda; Kabadayı, Hilal; Hanoğlu, Azmi; Hanoğlu, Duygu Yiğit; Yavuz, Dudu Özkum; Özek, Temel; Vatansever, Seda

doi:10.1080/13880209.2017.1296003

Cited by 61 publications

(39 citation statements)

References 36 publications

(32 reference statements)

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“…CPS oils were obtained by using supercritical CO2 extraction (Super critical fluid extraction 100-2-FMC system) (Thar Instruments Inc.) method. Fatty acid composition of spiny and thornless CPS oils were investigated by gas chromatography-mass spectrometry (GC-MS) analysis [3]. Colo-320 (ATCC catalogue: CCL 220) and Colo-741 cell lines (ECACC 93052621) are maintained in culture in RPMI-1640 medium (Biochrom, FG1215, Berlin, Germany), 10% FBS (Capricorn Scientific, FBS-11B, Ebsdorfergrund, Germany), 1% penicillin-streptomycin (Biochrom, A2213, Berlin, Germany) and 1% glutamine (EMD Millipore, K0282, Darmstadt, Germany) in a humidified atmosphere at 37 °C and 5% CO2.…”

Section: Methodsmentioning

confidence: 99%

Apoptotic Effects of Opuntia ficus indica L. Seed Oils on Colon Adenocarcinoma Cell Lines

Becer

Kabadayı

Meriçli

et al. 2018

The 2nd International Cell Death Research Congress

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Opuntia ficus indica L. fruit (cactus pear) seed oil is used in traditional and complementary therapies for its numerous health benefits. The aim of this study was to analyse of the fatty acid content and apoptotic induction effects of spiny and thornless Opuntia ficus indica L. seed (CPS) oils. Spiny and thornless Opuntia ficus indica L. seed oils obtained by supercritical CO2 extraction method and analyzed by GC-MS. Different concentrations of almond oils were incubated for 24 h and 48 h with Colo-320 and Colo-741 cells. Cell growth and cytotoxicity were measured by MTT assays. TUNEL assay was used to detect DNA fragmentation in both cell lines. Linoleic acid was dominant fatty acid followed by oleic acid, palmitic acid and elaidic acid in both type of seed oil. In MTT, spiny CPS oil was found to be active against Colo-320 and Colo-741 cells with 1:16 dilution for 48 h. Also, 1:8 and 1:16 dilutions of thornless CPS oil showed significant reduction in the number of viable cells in Colo-320 and Colo-741 cells, respectively. The number of TUNEL positive cells were significantly higher in Colo-320 cells treated with thornless CPS when compare with control group (p < 0.05).We conclude that thornless CPS oil may have anticancer effect on primer colon adenocarcinoma cell lines. The effect can be explained by inducing apoptosis. Thus, they could be a potential novel therapeutic agent in colon cancer therapy.

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Section: Methodsmentioning

confidence: 99%

Apoptotic Effects of Opuntia ficus indica L. Seed Oils on Colon Adenocarcinoma Cell Lines

Becer

Kabadayı

Meriçli

et al. 2018

The 2nd International Cell Death Research Congress

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“…The pharmacogenic and toxicity properties of all studied compounds also are computed and confirmed that most of all studied bioactive compounds especially, caulerpin obey Zaleya decandra [8] Ceramium virgatum [18] Ulva intestinalis [18] Fucus sp. [19] Chlorella vulgaris [20] Torreya Grandis [21] Hazelnut [22] Antimicrobial, [22] anticancer, [23] and antiviral activities [24] Saringosterols (2,3) Terrestrial plant Red macroalgae Green macroalgae Brown macroalgae…”

Section: Introductionmentioning

confidence: 99%

The inhibitory effect of some natural bioactive compounds against SARS-CoV-2 main protease: insights from molecular docking analysis and molecular dynamic simulation

Abdelrheem

Ahmed

El-Mageed

et al. 2020

Journal of Environmental Science and Health, Part A

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This work aimed at evaluating the inhibitory effect of ten natural bioactive compounds (1-10) as potential inhibitors of SARS-CoV-2-3CL main protease (PDB ID: 6LU7) and SARS-CoV main proteases (PDB IDs: 2GTB and 3TNT) by molecular docking analysis. The inhibitory effect of all studied compounds was studied with compared to some proposed antiviral drugs which currently used in COVID-19 treatment such as chloroquine, hydroxychloroquine, azithromycin, remdesivir, baloxvir, lopinavir, and favipiravir. Homology modeling and sequence alignment was computed to evaluate the similarity between the SARS-CoV-2-3CL main protease and other SARS-CoV receptors. ADMET properties of all studied compounds were computed and reported. Also, molecular dynamic (MD) simulation was performed on the compound which has the highest binding affinity inside 6LU7 obtained from molecular docking analysis to study it is stability inside receptor in explicit water solvent. Based on molecular docking analysis, we found that caulerpin has the highest binding affinity inside all studied receptors compared to other bioactive compounds and studied drugs. Our homology modeling and sequence alignment showed that SARS-CoV main protease (PDB ID: 3TNT) shares high similarity with 3CLpro (96.00%). Also, ADMET properties confirmed that caulerpin obeys Lipinski's rule and passes ADMET property, which make it a promising compound to act as a new safe natural drug against SARS-CoV-2-3CL main protease. Finally, MD simulation confirmed that the complex formed between caulerpin and 3CLpro is stable in water explicit and had no major effect on the flexibility of the protein throughout the simulations and provided a suitable basis for our study. Also, binding free energy between caulerpin and 6LU7 confirmed the efficacy of the caulerpin molecule against SARS-CoV-2 main protease. So, this study suggested that caulerpin could be used as a potential candidate in COVID-19 treatment.

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“…Fatty acid compounds in herbal plants have a role as chemopreventive agents or cause cell cycle inhibition and trigger apoptosis in cancer cells [41]. The results of this study revealed that a lower IC 50 value indicates a higher anticancer activity.…”

Section: Discussionmentioning

confidence: 67%

In Vitro Cytotoxic Activity of Rodent Tuber Mutant Plant (Typhonium Flagelliforme Lodd.) Against to McF-7 Breast Cancer Cell Line

Sianipar

Assidqi

Purnamaningsih

et al. 2019

Asian J Pharm Clin Res

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Objective: The objective of this study was to determine the new bioactive compounds through gas chromatography–mass spectrometry analysis and the cytotoxic activity of two rodent tuber mutant plants against breast cancer cells (MCF-7). Methods: The bioactive compounds in rodent tuber mutant plants were successfully increased by somaclonal variation using gamma rays irradiation technique. Further, the cytotoxicity activity of rodent tuber mutant plants was tested on breast cancer cell line (MCF-7) performed by 3-(4,5-dimethythiazol-2-yl)-2,5-diphenyltetrazolium bromide assay method. Results: This results study confirmed that the presence of phytochemical composition in the tuber of rodent tuber mutant plants KB 6–1–3–4 and KB 6–9–5 was found six bioactive compounds from fatty acid groups which have the potential as an anticancer compound, such as octadecanoic acid, hexadecanoic acid, hexadecanoic acid methyl ester, 9-octadecanoic acid, linolelaidic acid methyl ester, and butanoic acid. The results showed that extracts from rodent tuber mutant plants had a cytotoxicity effect on MCF-7 cancer cells with half maximal inhibitory concentration (IC50) values that were lower than the control (mother plant). In vitro tests of KB 6–1–3–4 and KB 6–9–5 against MCF-7 cancer cell lines have IC50 values of 12.482 μg/mL and 7.043 μg/mL, respectively, while it had a lower cytotoxicity effect with the IC50 value of control plant was 19.113 μg/mL. The mutant plants of KB 6-9-5 have 3 times more effective than control. Conclusion: The results of this study clearly indicated that rodent tuber mutant plants have shown promising as an anticancer drug on breast cancer.

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Fatty acid composition and anticancer activity in colon carcinoma cell lines of Prunus dulcis seed oil

Cited by 61 publications

References 36 publications

Apoptotic Effects of Opuntia ficus indica L. Seed Oils on Colon Adenocarcinoma Cell Lines

Apoptotic Effects of Opuntia ficus indica L. Seed Oils on Colon Adenocarcinoma Cell Lines

The inhibitory effect of some natural bioactive compounds against SARS-CoV-2 main protease: insights from molecular docking analysis and molecular dynamic simulation

In Vitro Cytotoxic Activity of Rodent Tuber Mutant Plant (Typhonium Flagelliforme Lodd.) Against to McF-7 Breast Cancer Cell Line

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