In Vitro Cytotoxic Activity of Rodent Tuber Mutant Plant (Typhonium Flagelliforme Lodd.) Against to McF-7 Breast Cancer Cell Line

Abstract

Objective: The objective of this study was to determine the new bioactive compounds through gas chromatography–mass spectrometry analysis and the cytotoxic activity of two rodent tuber mutant plants against breast cancer cells (MCF-7).
 Methods: The bioactive compounds in rodent tuber mutant plants were successfully increased by somaclonal variation using gamma rays irradiation technique. Further, the cytotoxicity activity of rodent tuber mutant plants was tested on breast cancer cell line (MCF-7) performed by 3-(4,5-dimethythiazol-2-yl)-2,5-diphenyltetrazolium bromide assay method.
 Results: This results study confirmed that the presence of phytochemical composition in the tuber of rodent tuber mutant plants KB 6–1–3–4 and KB 6–9–5 was found six bioactive compounds from fatty acid groups which have the potential as an anticancer compound, such as octadecanoic acid, hexadecanoic acid, hexadecanoic acid methyl ester, 9-octadecanoic acid, linolelaidic acid methyl ester, and butanoic acid. The results showed that extracts from rodent tuber mutant plants had a cytotoxicity effect on MCF-7 cancer cells with half maximal inhibitory concentration (IC50) values that were lower than the control (mother plant). In vitro tests of KB 6–1–3–4 and KB 6–9–5 against MCF-7 cancer cell lines have IC50 values of 12.482 μg/mL and 7.043 μg/mL, respectively, while it had a lower cytotoxicity effect with the IC50 value of control plant was 19.113 μg/mL. The mutant plants of KB 6-9-5 have 3 times more effective than control.
 Conclusion: The results of this study clearly indicated that rodent tuber mutant plants have shown promising as an anticancer drug on breast cancer.

Assessment of Wheat Germ Oil Role in the Prevention of Induced Breast Cancer in Rats

“…29 It has been demonstrated that four compounds, oleic acid, palmitic acid, linoleoyl chloride, and hexadecanoic acid, methyl ester, have potential anticancer activity. Two of these four compounds, oleic acid and palmitic acid, have the most anticancer activity, as reported by Liu et al 27 and Sianipar et al, 30 respectively. Table 3 summarizes that oleic acid and palmitic acid have higher concentrations in WGO than in WGP.…”

Effect of Light Exposure on Secondary Metabolite Production and Bioactivities of Syncephalastrum racemosum Endophyte

“…35 Meanwhile, octadecanoic acid, methyl hexadecanoate, hexadecanoic acid, and octadec-9-enoic acid were believed to have potential as anticancer. 36 Based on this study, the anticancer activities could be contributed from the detected chemical compounds in the extracts derived from dark fermentation as analyzed by GC-MS. Searching other potency as antibacterial agent was also conducted by testing the extracts against S. aureus as gram-positive bacteria and E. coli as gram-negative bacteria. Similar with antioxidant potency, extract from fermentation under light condition showed better activity against S. aureus compared to dark condition, although only slight differences in viability were observed (at the concentration of 250, 125 and 31.25 µg/mL).…”

Characterization and Investigation of Stigmasterol Isolated from Rodent Tuber Mutant Plant (<em>Typhonium flagelliforme</em>), Its Molecular Docking as Anticancer on MF-7 Cells

“…In comparison, the ethanol extract has an IC50 value of 1.60 µg/mL [9]. This indicated that the cytotoxic activity of T. flagelliforme mutant extract was three times more effective than wild-type extract, with an IC50 value of 19.11 µg/mL [8].…”

“…T. flagelliforme mutant plant received Plant Variety Protection by the Indonesian Ministry of Agriculture in 2020. T. flagelliforme mutant plant has been shown to have bioactive compounds, and its potential as an anticancer agent has increased [6][7][8]. The ethyl acetate fraction of the superior T. flagelliforme mutant has potential as an anticancer with an IC50 value of 1.09 µg/mL.…”