The effect of Colchicum pusillum in human colon cancer cells via Wnt/β-catenin pathway

Becer, Eda; Hanoğlu, Duygu Yiğit; Kabadayı, Hilal; Hanoğlu, Azmi; Vatansever, Seda; Yavuz, Dudu Özkum; Meriçli, Filiz; Meriçli, Ali H.

doi:10.1016/j.gene.2018.11.047

Cited by 10 publications

(7 citation statements)

References 20 publications

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“…However, it inhibits the growth of lung, breast, endometrial, renal, and gastric cancers, and there are few studies about its effect on CRC development. A significant increase of Wnt7a expression was detected in ulcerative colitis specimens and CRC cells, implying a potential carcinogenic effect ( 49 , 55 ), whereas Becer et al found that Colchicum pusillum exerted anticancer activities through activating the Wnt7a/β-catenin pathway, and another study also proved that the loss of Wnt7a expression contributed to tumor progression and predicted a poor prognosis of CRC, which indicates a protective role of Wnt7a during CRC carcinogenesis ( 56 , 111 ). Thus, the exact role of Wnt7a in CRC progression still needs to be studied further.…”

Section: Roles Of Wnts In Tumorigenesis and The Progression Of Crcmentioning

confidence: 99%

Emerging Roles of Wnt Ligands in Human Colorectal Cancer

et al. 2020

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Colorectal cancer (CRC) is the fourth leading cause of cancer death worldwide, and constitutive activation of the Wnt signaling pathway is universal in most CRC cases. Wnt ligands (Wnts) are secreted glycoproteins and fundamentally essential for the transduction of Wnt signaling pathway. However, the 19 members of Wnts in humans imply a daunting complexity of Wnt signaling and biological effects, and our understanding of their roles in CRC tumorigenesis is still quite rudimentary. This review will give an overview of the structural characteristics and maturation process of Wnts. The expression pattern of all human Wnts in CRC tissues, including Wnt1, Wnt2,

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Section: Roles Of Wnts In Tumorigenesis and The Progression Of Crcmentioning

confidence: 99%

Emerging Roles of Wnt Ligands in Human Colorectal Cancer

et al. 2020

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“…Finally, UV absorbances of them were measured at 352 nm, and the graph of the calibration was drawn. Moreover, these extracts were used in HPLC for the determination of any tropolone alkaloids (14,15).…”

Section: Total Analysis Of Phytoconstituentsmentioning

confidence: 99%

Phytochemical Profiles, Physicochemical Analysis, and Biological Activity of Three Colchicum Species

Davoodi

Azadbakht

Hosseinimehr

et al. 2021

Jundishapur J Nat Pharm Prod

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Background: Colchicum is a genus of the Colchicaceae family with various isolated compounds, especially tropolone alkaloids and flavonoids, which are used for osteoarthritis, gout, cancer, inflammatory diseases, jaundice, and sexual impotence in different societies. Objectives: The current study aimed to evaluate the phytochemical and physicochemical properties and anti-inflammatory activities of three Colchicum species. Methods: Total tropolone alkaloid, total phenolic/total tannin, and total flavonoid contents were determined using acidic potassium dichromate, Folin-Ciocalteu, and aluminum chloride methods, respectively. Moreover, the HPLC method was used for identification and quantitation purposes of tropolone alkaloids. Physicochemical properties of three Colchicum species, including macroscopic and organoleptic properties, solubility, foreign matter, ash values, and heavy metal contents, were evaluated. Besides, in vitro anti-inflammatory activities of the corms also were determined using the protein denaturation technique as a rapid screening method. Results: The highest levels of tropolone alkaloid, phenolic compounds, tannins, and flavonoids were observed in C. autumnale, C. speciosum, and C. robustum, respectively. HPLC analysis indicated the presence of colchicine, demecolcine, 2-demethyl colchicine, 3-demethyl colchicine, colchicoside, colchifoline, cornigerine, and N-deacetyl-N-formyl colchicine in these Colchicum species. The physicochemical properties of C. speciosum and C. robustum corms are appropriate compared to the standard Colchicum autumnale corm. Moreover, all Colchicum species exhibited high anti-inflammatory activities compared to standard drugs. Conclusions: This study demonstrated that the corm of the Colchicum species contained similar main compounds with different amounts, as well as appropriate physicochemical properties. Moreover, the valuable biological effects of these plants stimulate the cultivations on a large scale.

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“…The medicinal value of the Colchicum species is ascribed to the presence of tropolonic alkaloids, colchicinoids, mainly colchicine [6]. Ancient Greek physicians used the plant as a therapeutic agent for gout.…”

Section: Introductionmentioning

confidence: 99%

Chemical Profiling and Biological Properties of Extracts from Different Parts of Colchicum Szovitsii Subsp. Szovitsii

et al. 2019

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Like other members of the Colchicum genus, C. szovitsii subsp. szovitsii is also of medicinal importance in Turkish traditional medicine. However, its biological properties have not been fully investigated. Herein, we focused on the evaluation of the in vitro antioxidant and enzyme inhibitory effects of flower, root and leaf extracts, obtained using different extraction methods. In addition, a comprehensive (poly)-phenolic and alkaloid profiling of the different extracts was undertaken. In this regard, ultra-high-performance liquid chromatography coupled with quadrupole time-of-flight mass spectrometry (UHPLC-QTOF-MS) allowed us to putatively annotate 195 polyphenols and 87 alkaloids. The most abundant polyphenols were flavonoids (83 compounds), whilst colchicine and 2-demethylcolchicine were some of the most widespread alkaloids in each extract analyzed. However, our findings showed that C. szovitsii leaf extracts were a superior source of both total polyphenols and total alkaloids (being, on average 24.00 and 2.50 mg/g, respectively). Overall, methanolic leaf extracts showed the highest (p < 0.05) ferric reducing antioxidant power (FRAP) reducing power (on average 109.52 mgTE/g) and 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical scavenging (on average 90.98 mgTE/g). Interestingly, each C. szovitsii methanolic extract was more active than the water extracts when considering enzymatic inhibition such as against tyrosinase, glucosidase, and acetylcholinesterase (AChE). Strong correlations (p < 0.01) were also observed between polyphenols/alkaloids and the biological activities determined. Multivariate statistics based on supervised orthogonal projections to latent structures discriminant analysis (OPLS-DA) allowed for the detection of those compounds most affected by the different extraction methods. Therefore, this is the first detailed evidence showing that C. szovitsii subsp. szovitsii might provide beneficial effects against oxidative stress and the associated chronic diseases. Nevertheless, the detailed mechanisms of action need to be further investigated.

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The effect of Colchicum pusillum in human colon cancer cells via Wnt/β-catenin pathway

Cited by 10 publications

References 20 publications

Emerging Roles of Wnt Ligands in Human Colorectal Cancer

Emerging Roles of Wnt Ligands in Human Colorectal Cancer

Phytochemical Profiles, Physicochemical Analysis, and Biological Activity of Three Colchicum Species

Chemical Profiling and Biological Properties of Extracts from Different Parts of Colchicum Szovitsii Subsp. Szovitsii

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