The hemolytic effects of 27 organotin compounds, which are environmental pollutants, were studied with rabbit erythrocytes. Various EC50 values caused by differences in their chemical structures were observed. The hemolytic activities of tri‐n‐butyltins and triphenyltins were higher than that of sodium‐n‐dodecyl sulfate, and the hemolysis by tri‐n‐butyltin chloride proceeded rapidly. Tri‐n‐butyltin chloride showed the highest hemolytic activity (EC50 = 7.48 μM). Methyltin compounds were less active (EC50>364 μM) than any other organotin compound tested. No significant difference in hemolytic activity based on divergences of the anionic functional group which was attached to each triorganotin compound was observed. A structure–activity relationship study was carried out in order to predict EC50 values of a series of tested organotin compounds, using various descriptors which represent their physicochemical properties or molecular structures. In the multiparametric regression analysis, the best regression equation (r= 0.854) for estimation of their hemolytic effects was obtained by adopting the descriptors Index Value (IV), Mean Information Index (I Dw) and Molecular Connectivity Index (0χp).
Various organotin compounds caused strong hemolysis of erythrocytes. To investigate the mechanism of hemolysis, spin-labeling techniques with electron spin resonance (ESR) spectroscopy were used and the relationship between hemolytic toxicity and signal intensity was investigated. Two kinds of spin-labeled stearic acid in which the paramagnetic center was located at different sites on the alkyl chain (5-and 12-doxyl-stearic acids; 5-and 12-NS) were used. It became clear that the decrease of ESR signal intensity was related to the hemolytic toxicity of the organotin compounds.
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