Relationship between Hemolytic Toxicity and Signal Intensity of Various Organotin Compounds by a Spin‐labeling Technique

Abstract

Various organotin compounds caused strong hemolysis of erythrocytes. To investigate the mechanism of hemolysis, spin-labeling techniques with electron spin resonance (ESR) spectroscopy were used and the relationship between hemolytic toxicity and signal intensity was investigated. Two kinds of spin-labeled stearic acid in which the paramagnetic center was located at different sites on the alkyl chain (5-and 12-doxyl-stearic acids; 5-and 12-NS) were used. It became clear that the decrease of ESR signal intensity was related to the hemolytic toxicity of the organotin compounds.

Different Effects of Di- and Triphenyltin Compounds on Lipid Bilayer Dithionite Permeabilization

“…For this and other reasons, we became in terested in the effect of both compounds on liposome membranes. Only a limited number of reports concerning organotin biological potency is available, and studies on model membranes that address questions regarding the molecular basis of the biological effect of these compounds (Aldridge Street, 1964;Selwyn et al, 1970;Selwyn, 1976;Arkagawa and Wada, 1984;Ambrosini et al, 1996;Cullen et al, 1997;Sato et al, 1997). The objective of the study was to determine the difference be tween the effects of di-and triphenyltin on S20 4~2 ion permeation across a phosphatidylcholine lipo some membrane, and the concomitant difference in localization of the two phenyltins in the mem brane.…”