Objective: To determine the occurrence of CTX-M producing Escherichia coli (E. coli) from cattle feces in Bogor slaughterhouse, Indonesia. Methods: A total of 220 cattle feces samples were collected from Bogor slaughterhouse from March to April 2015. Presence of extended-spectrum beta-lactamase (ESBL) producing E. coli was detected by disc diffusion test based on the recommendation from Clinical and Laboratory Standards Institute (2014). Bacterial strains which were confirmed as producing ESBLs were further analyzed for the presence of bla genes of the ESBL by PCR. Results: The results showed that CTX-M producing E. coli isolates were detected in 19 samples from 220 samples (8.6%). The β-lactamase genes detected were CTX-M-1 (n = 10) and CTX-M-9 (n = 9). All of the CTX-M producing E. coli isolates showed multidrug resistance phenotypes to at least four antibiotics. The highest incidence of antibiotics resistance was showed to ampicillin (100.0%), cefotaxime (100.0%), and cefpodoxime (100.0%), followed by streptomycin (84.3%), trimethoprim-sulfamethoxazole (73.7%), erythromycin (52.6%), kanamycin (26.3%), doxycycline (10.5%), and ceftazidime (0.0%). Conclusions: Detection of CTX-M-producing E. coli in cattle feces raises important questions as they can represent a potential risk factor to public health.

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Parameter untuk mengetahui hewan sapi sempurna setelah disembelih yaitu dengan melihat refleks kelopak mata dan atau waktu henti darah memancar. Menurut EFSA (2004) kematian merupakan suatu keadaan yang ditandai dengan respirasi fisiologis dan sirkulasi darah telah berhenti sebagai akibat dari pusat sistem tersebut di batang otak secara permanen kehilangan fungsi karena kekurangan oksigen dan energi. Waktu henti darah memancar merupakan indikasi bahwa jantung sudah tidak dapat memompa darah keluar dari tubuh karena tidak ada lagi asupan oksigen darah dalam jantung, sehingga hewan tersebut dapat dikatakan mati. Tujuan dari penelitian ini untuk menghitung waktu henti darah memancar pada penyembelihan sapi dengan metode pemingsanan dan tanpa pemingsanan yang dipotong di rumah potong hewan ruminansia besar (RPHRB), sehingga diperoleh data rataan waktu hewan mati sempurna. Tiga puluh ekor sapi Brahman Cross dibagi menjadi 2 kelompok perlakuan yaitu, sebanyak 15 ekor yang disembelih dengan pemingsanan (kelompok 1) dan sebanyak 15 ekor yang disembelih tanpa pemingsanan (kelompok 2). Waktu henti darah memancar dihitung sesaat setelah hewan disembelih sampai darah berhenti memancar. Hasil dari penelitian diperoleh rataan waktu henti darah memancar pada sapi yang dipingsankan sebelum disembelih adalah sebesar 3,02 menit dan rataan waktu henti darah memancar pada sapi yang disembelih tanpa pemingsanan adalah sebesar 2,13 menit. Selang waktu henti darah memancar antara sapi yang dipingsankan dengan sapi yang tidak dipingsankan sebelum disembelih adalah 53,4 detik. Waktu henti darah memancar dipengaruhi oleh perlakuan hewan sebelum pemotongan, yaitu dengan atau tanpa pemingsanan.Kata kunci: Pemingsanan, sapi, tanpa pemingsanan, waktu henti darah (The Perfect Cow Died after Slaughtered by Stunning and Non Stunning Methods According to Gushing Blood Downtime)Palpebra reflex and gushing blood downtime can be used as parameters to see animals death after slaughtered. Stop bleeding time was an indication that the heart is unable to pump blood out of the body due to no more oxygen in the blood of the heart, so that the cattle can be said has been dead perfectly. The aims of this study was to calculate the stop bleeding time of cattle slaughtered by stunning and non stunning methods, thus obtained the avaraging data of perfectly death time of animals. Thirty catlles’s Brahman Cross divided into two treatment groups, firstly 15 cattle’s were slaughtered by stunning method (group 1) and the second one 15 cattle’s were slaughtered by non stunning method (group 2). Blood gushing downtime was calculated immediately after the animal is slaughtered until the blood stops radiating. The results showed the average blood gushing downtime in cattles that were stunning before slaughtered is 3.02 minutes and the average time to stop blood gushing in cattles of non stunning group is 2.13 minutes. The interval blood gushing downtime between the cattles slaughtered by stunning and non stunning was 53.4 seconds. Blood gushing downtime was affected by the treatment of animals before they were slaughtered.Keywords: cattle, gushing blood downtime, non stunning, stunning.

Mastitis is udder inflammation that can be caused by the entry of pathogenic bacteria through the teat. One of the treatments that can prevent bacteria infection is teat dipping. This research was aimed to identify the effect of teat dipping after milking to the presence of pathogenic bacteria that cause subclinical mastitis (Staphylococcus aureus, Streptococcus agalactiae, and Escherichia coli) and the presence of pathogenic bacteria that cause subclinical mastitis by quarter of udder. Milk samples were taken from normal lactation period of dairy cows, clinically healthy, and positive subclinical mastitis status when the intervention was applied. Sixty seven samples of quarters from 18 dairy cows were identified to the presence of pathogenic bacteria that cause subclinical mastitis before teat dipping and at 1st, 2nd, and 3rd months after teat dipping. Identification of the presence of pathogenic bacteria was done by culturing samples on selective agar media. The presence of common pathogenic bacteria, Staphylococcus aureus, and Escherichia coli decreased significantly after teat dipping, while Streptococcus agalactiae and other pathogenic bacteria did not decrease significantly. The presence of pathogenic bacteria based on the quarter of udder did not show the significance difference. Application of teat dipping after milking was able to decrease the presence of pathogenic bacteria that cause subclinical mastitis. Keywords: pathogenic bacteria, subclinical mastitis, teat dipping AbstrakMastitis merupakan peradangan ambing yang dapat disebabkan oleh masuknya bakteri melalui lubang puting. Salah satu kegiatan yang dapat mencegah infeksi bakteri ke dalam jaringan internal ambing adalah celup puting setelah pemerahan. Penelitian ini bertujuan mengidentifikasi pengaruh celup puting setelah pemerahan terhadap keberadaan bakteri patogen, Staphylococcus aureus, Streptococcus agalactiae, dan Escherichia coli pada sapi perah positif mastitis subklinis serta menganalisa keberadaan bakteri tersebut berdasarkan posisi puting. Sampel susu yang digunakan berasal dari sapi perah dalam masa laktasi normal, sehat secara klinis, dan positif menderita mastitis subklinis sebelum diberi perlakuan celup puting. Sejumlah 67 sampel kuartir dari 18 ekor sapi perah diidentifikasi terhadap keberadaan bakteri patogen, Staphylococcus aureus, Streptococcus agalactiae, dan E. coli JSV 35 (1), Juni 2017

provide a promising prospect for the people of East Barito. Unfortunately, many traditional markets in East Barito do not yet have special kiosks for the sale of pork. Currently, pork in East Barito sold on the roadside of the Tamiang Layang Market. The condition can cause the pork directly exposed to the sun, rain, dust, and other contaminants from the highway, which further may affect the pork quality. Food that sold openly on the roadside has a higher chance of being contaminated by coliform bacteria and pathogenic bacteria such as Escherichia coli, Salmonella sp., Staphylococcus aureus, Bacillus cereus, Clostridium perfringens, and Vibrio cholerae (Cho et al., 2011; Hanashiro et al., 2005; Mankee et al., 2005). Contamination of pathogenic bacteria in meat may result in foodborne disease (CDC 2018). Escherichia coli (E. coli) is a pathogenic bacteria causing foodborne disease that is harmful to human health. E. coli is also known as commensal bacteria which commonly

The purpose of this study was to obtain Escherichia coli isolates from 11 se’i meat sellers in Kupang City and to observe their resistance to13 types of antibiotics. Escherichia coli were isolated and identified based on SNI 2897: 2008. Antibiotic resistance of the Escherichia colii isolates was determined using the Kirby-Bauer method. The results showed that 13 isolates (39.39%) of Escherichia coli in se’i meat samples had a high level of resistance to erythromycin (100%), tetracycline (76.92%), and doxycycline (61.54%). Isolates demonstrated increased resistance to streptomycin (46.15%), cephalothin (38.46%), trimethoprim-sulfamethoxazole (38.46%), amoxicillin (30.77%), chloramphenicol (30.77%), and choline sulfate (30.77%). The antibiotics nalidixic acid and ciprofloxacin demonstrated low Escherichia coli resistance (7.69%). Escherichia coli are sensitive to cefotaxime and gentamicin. A total of 12 isolates (92.31%) experienced MDR. The presence of non-MDR and MDR resistant Escherichia coli in se’i meat can seriously threaten community health.

This study was aimed to determine Enterobacteriaceae contamination that were resistant against aantibiotics in duck meat which was related with food safety for the consumers. Total of 52 samples of ducks were taken from 5 subdistricts in Bogor District, i.e., Ciomas, Gunung Sindur, Klapanunggal, Jasinga, and Parung Panjang. This sample size was calculated based on the assumption of 95% confidence level, 50%predicted prevalence, and 10% standard error. The ducks were collected from small-scale farmers which supplied duck meat to markets, so the ducks were slaughtered by them. The samples of duck meat were handled aseptically and transported in cool box to the laboratory. The total of Enterobacteriaceae was examined using plate count method with violet red bile glucose agar according to Kornacki and Johnson (2001). The examination of antibiotic resistance was done using disc diffusion method and the results were interpreted according to the Clinical and Laboratory Standards Institute (CLSI) Guidelines (CLSI 2012). The data was analysed descriptively. The results showed that the total of Enterobacteriacea were 79679 ± 260463 cfu/g. The majority of Enterobacteriaceae isolated from duck meat (84.6%) showed resistant against 5 of antibiotic (gentamycin, kanamycin, tetracycline, trimethoprim, and ampicillin). The Enterobacteriaceae had resistance against tetracycline (93.2%), trimethoprim (88.6%), and ampicillin (81.8%). Nevertheless, this family was still sensitive againts kanamycin (34.1%) and gentamycin (15.9%). The isolated bacteria (69.2%) showed multi-antibiotic 251

The adulteration of beef using wild boar meat in the city of Bogor has been a serious concern in the society. Monitoring and surveillance of beef products are needed to ensure the halal of animal products and to prevent the transmission risk of zoonotic diseases from wild boar meat to humans. The purpose of this study was to analyse the data of Dinas Pertanian Kota Bogor related to meat adulteration in 2013-2017. The case study approach was used in this research by conducting a collection of primary and secondary data. The primary data obtained from an indepth interview with the chief of veterinary public health, processing, and marketing of livestock products of Dinas Pertanian Kota Bogor. The secondary data were obtained from monitoring and surveillance report of Dinas Pertanian Kota Bogor in 2013-2017. The results showed that 7.86% (3/33 samples) of beef samples contained wild boar meat during the 2013-2017 period. The adulterated beef was found mainly from the meat kiosks in traditional markets. In conclusion, monitoring and surveillance related to meat adulteration problem is needed to be improved especially in the traditional market.