Multidrug resistance extended spectrum β-lactamase and AmpC producing  Escherichia coli  isolated from the environment of Bogor Slaughterhouse, Indonesia

Sudarwanto, Mirnawati; Lukman, Denny Widaya; Purnawarman, Trioso; Latif, Hadri; Pisestyani, Herwin; Sukmawinata, Eddy

doi:10.1016/j.apjtb.2017.07.012

Cited by 15 publications

(13 citation statements)

References 20 publications

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“…A relatively high level resistance of ESBLproducing bacteria was observed on aztreonam, trime thoprim/sulfamethoxazole, gentamicin, and ciprofloxacin, by 86.36%, 77.27%, 72.73%, and 68.18% consecutively, as the previous study with relatively high level of resistance of ciprofloxacin (50.0%), gentamicin (60.0%), trimethoprimsulfamethoxazole (60%, and ciprofloxacin (40.0%) (Cormican et al, 1996;et alKürekci et al, 2017;Sudarwanto et al, 2017). Other antibiotics such as ertapenem and mempenem from carbapenem class, amikacin (aminoglycoside class), and tigecycline (tetracycline class) still show high level of sensitivity (100%), according to mechanism of action of ESBL which hydrolyze penicilin class antibiotics, first, second, and third generations of cephalosporin, and monobactam antibiotic class, but inactive on carbapenem class (imipenem, meropenem, ertapenem) (Lim et al, 2013;Biutifasari, 2018).…”

Section: Resultssupporting

confidence: 75%

“…Accredited: 36a/E/KPT/2016 http://buletinpeternakan.fapet.ugm.ac.id/ causes resistance to wider spectrum on third generation of cephalosporins and mobobactams; thus, the antiobiotic becomes ineffective. ESBLproducing bacteria can also be resistant to wide array of antibiotic class: aminoglycoside, tetracycline chloramphenicol, and sulfamethoxazole-trimethoprim (Brower et al, 2017;Sudarwanto et al, 2017). Incidence on multidrug resistance to third generation cephalosporins and other antibiotic classes are often found in ESBL-producing bacteria (Masruroh et al, 2016).…”

Section: Issn-0126-4400/e-issn-2407-876xmentioning

confidence: 99%

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Resistance Profile of Extended Spectrum Beta Lactamase-Producing Escherichia coli Bacteria using Vitek® 2 Compact Method

et al. 2020

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This study aimed to determine the resistance profile and the nature of multidrug resistance in Extended Spectrum Beta Lactamase (ESBL)-producing Escherichia coli (E.coli) against several classes of antibiotics. Positive isolates of ESBL-producing E.coli were tested for antibiotic sensitivity using the VITEK® 2 compact method which then analyzed automatically. The results showed an antibiotic resistance profile against ESBL-producing E.coli showed the highest level of antibiotics in beta lactam, amoxicillin, ampicillin, cefazolin, cefotaxime, and ceftriaxone at 100%. Subsequent results found a relatively high level of resistance in the antibiotics aztreonam (86.36%), trimethoprim/sulfamethoxazole (77.27%), gentamicin (72.73%), and ciprofloxacin (68.18%). Antibiotics from carbapenem groups such as ertapenem and memenem, and antibiotics from the aminoglycosides (amicasin) and tigecycline groups of tetracycline still showed a high sensitivity level of 100%. The most common resistance patterns found in ESBL-producing E.coli isolates are AM/AMP/KZ/CTX/CRO/ATM/GM/CIP as much as 22.73%, and AM/AMP/KZ/CTX/CRO/ATM/GM/CIP/SXT patterns of 18.2%. The results of multi-class antibiotic resistance showed that 86.36% had multidrug resistance. The highest multidrug resistance pattern in ESBL-producing E.coli occurred with a BL/AG/Q/SP pattern of 50%. Other patterns of multidrug resistance in ESBL-producing E.coli that can be found in this study are, the BL/AG/Q/SP pattern is 18.20%, the BL/AG/Q/SP pattern is 13.64%, and the BL/AG/Q pattern is 4.55%. The high profile of resistance and the nature of multidrug resistance in ESBL-producing E.coli has the potential to spread these resistant genes, thus risking the use of antibiotics as a public health therapy and animal health, therefore further evaluation and control are needed.

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Section: Resultssupporting

confidence: 75%

Section: Issn-0126-4400/e-issn-2407-876xmentioning

confidence: 99%

Resistance Profile of Extended Spectrum Beta Lactamase-Producing Escherichia coli Bacteria using Vitek® 2 Compact Method

et al. 2020

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“…Contamination of E. coli resistant bacteria can occur from slaughterhouse to consumers table. Recent study showed that E. coli was isolated from the environment of Bogor Slaughterhouse, Indonesia and resistant to penicillin G (100.0%), streptomycin (100.0%), followed by gentamicin (60.0%), trimethoprim-sulfamethoxazole (60.0%), tetracycline (40.0%), ciprofloxacin (40.0%), enrofloxacin (20.0%) (Sudarwanto et al, 2017).…”

Section: Discussionmentioning

confidence: 99%

“…The previous study reported E. coli (80%) isolated from the environment of Bogor slaughterhouse were resistant to at least three antibiotic classes. The spread of these organisms could be a problem of food safety, human, animals, and other pathogen bacteria (Sudarwanto et al, 2017). The increasing occurrence of MDR in E. coli in human and veterinary medicine has become an alarming issue (Poirel et al, 2018).…”

Section: March 2020 | Volume 8 | Issue 3 | Page 282mentioning

confidence: 99%

Antibiotic Resistance in Escherichia coli Isolated from Döner Kebab Sold in Dramaga Bogor, Indonesia

Sari¹,

Pisestyani²,

Lukman³

2020

Adv. Anim. Vet. Sci.

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This study was aimed to determine the occurrence of antibiotic resistance in Escherichia coli (E. coli) isolated from Döner kebab sold in Dramaga Bogor, West Java Province, Indonesia. A total of 43 samples of Dὂner kebab meat were taken from all of the vendors in the radius of 2 km around the border of IPB University campus, Dramaga Bogor. Isolation and identification of E. coli were referred to the Guideline for Laboratory Analysis on Examination of Microbial Contamination in Meat, Egg, and Milk in SNI 2897:2008 issued by the National Standardization Agency of Indonesia. The resistance against nine antibiotics were tested using Kirby-Bauer disk diffusion method based on the standard of Clinical Laboratory Standards Institute (CLSI) in 2018. The result showed that nine samples were positive containing E. coli (20.9%) and 84.2% of E. coli isolates were resistant to eight antibiotics: gentamicin (57.9%), ampicillin (26.3%), cefotaxime, trimethoprim-sulfamethoxazole, and ciprofloxacin (21.1%), amoxicillin-clavulanate (10.5%), enrofloxacin, and oxytetracycline (5.3%). All of E. coli isolates were susceptible to chloramphenicol (100%). There were 21.1% of isolates also resistant to three or more classes of antibiotics that were known as multi-drug resistant (MDR) with different resistant patterns.The presence of E. coli resistant antibiotic in kebab meat could results serious problems in human health.

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“…Bakteri ini sangat berbahaya karena mampu menyebar malalui vektor lain seperti makanan dari hewani yang berasal dari rumah potong yang telah terkontaminasi. Hasil penelitian Sudarwanto et al (2017) (Sibero et al 2018 1,2 ).…”

Section: Pendahuluanunclassified

Karakterisasi Senyawa Bioaktif Kapang Laut Trichoderma asperellum MT02 dengan Aktivitas Anti-Extended Spectrum β-Lactamase (ESBL) E. coli

et al. 2019

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The Trichoderma asperellum MT02 has been reported to has antibacterial activity against the Extended Spectrum β-Lactamase (ESBL) E. coli based on the screening results through an agar plug method. This study aimed to evaluate the antibacterial activity of T. asperellum MT02 and characterize the composition of the bioactive compounds group possessed in its crude extract. The isolate was cultured in the Malt Extract Broth (MEB) media (static, 27 oC, 15 days). The intracellular metabolites from mycelium were extracted using methanol while extracellular metabolites from broth media were extracted using ethyl acetate. The antibacterial activity of crude extracts was tested using the paper disc diffusion method while bioactive compounds were characterized using the phytochemical method. The results showed that the antibacterial activity of the broth media extract performed a greater activity than the crude extract from the mycelium. The crude extract from mycelia only contained flavonoid and phenol hydroquinone compounds while the crude extract from broth media contains alkaloids, flavonoids, phenols hydroquinone and saponins. Kapang Trichoderma asperellum MT02 telah dilaporkan memiliki potensi sebagai penghasil senyawa antibakteri melawan Extended Spectrum β-Lactamase (ESBL) E. coli berdasarkan hasil penapisan melalui metode agar plug. Penelitian ini bertujuan untuk mengetahui aktivitas antibakteri ekstrak kasar kapang T. asperellum MT02 serta komposisi golongan senyawa bioaktif yang dimiliki. Kapang dikultur pada media Malt Extract Broth (MEB) (statis, 27 oC, 15 hari) di mana metabolit intraseluler dari miselium diekstrak menggunakan metanol sedangkan metabolit ekstraseluler dari media kaldu diekstrak menggunakan etil asetat. Aktivitas antibakteri ekstrak kasar diuji menggunakan metode difusi kertas cakram sedangkan senyawa bioaktif dikarakterisasi menggunakan metode fitokimia. Hasil penelitian menunjukkan bahwa aktivitas antibakteri asal ekstrak media kaldu lebih baik dibandingkan ekstrak kasar asal miselium kapang. Ekstrak kasar kapang asal miselia hanya mengandung senyawa golongan flavonoid dan fenol hidrokuinon sedangkan ekstrak kasar asal media kaldu mengandung alkaloid, flavonoid, fenol hidroquinon dan saponin.

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Multidrug resistance extended spectrum β-lactamase and AmpC producing Escherichia coli isolated from the environment of Bogor Slaughterhouse, Indonesia

Cited by 15 publications

References 20 publications

Resistance Profile of Extended Spectrum Beta Lactamase-Producing Escherichia coli Bacteria using Vitek® 2 Compact Method

Resistance Profile of Extended Spectrum Beta Lactamase-Producing Escherichia coli Bacteria using Vitek® 2 Compact Method

Antibiotic Resistance in Escherichia coli Isolated from Döner Kebab Sold in Dramaga Bogor, Indonesia

Karakterisasi Senyawa Bioaktif Kapang Laut Trichoderma asperellum MT02 dengan Aktivitas Anti-Extended Spectrum β-Lactamase (ESBL) E. coli

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