UVA can cause oxidative stress and photoaging of cells. We established a UVA-induced oxidative stress model of human fibroblasts and focused on the antioxidant and anti-photoaging ability of Lactobacillus plantarum fermented Rhodiola rosea. Compared with the unfermented Rhodiola rosea, Lactobacillus plantarum fermented Rhodiola rosea has better DPPH free radical and hydroxyl free radical scavenging ability, significantly reduces the content of reactive oxygen species (ROS), and improves the antioxidant level. Further studies have shown that the Lactobacillus plantarum fermented Rhodiola rosea can activate the Nrf2/Keap1 signaling pathway and up-regulate heme oxygenase-1 (HO-1), NAD(P)H quinone dehydrogenase 1 (NQO1), catalase (CAT) and glutathione Peptide peroxidase (GSH-Px), and protect fibroblasts from oxidative stress caused by UVA. On the other hand, Lactobacillus plantarum fermented Rhodiola rosea significantly reduces the activity of metalloproteinases in the cell, thereby increasing the collagen and elastin in the cell, alleviating the photoaging caused by UVA. Finally, we concluded that the antioxidant capacity and anti-photoaging ability of Lactobacillus plantarum fermented Rhodiola rosea are better than that of unfermented Rhodiola rosea.

The exopolysaccharide secreted by Lacticaseibacillus paracasei subsp. paracasei SS-01 strain (LP-EPS) is isolated and purified from yogurt. It is a polysaccharide with a branched and multi-stranded structure, which exists in a smooth rod-like or cloud-like state, and possesses a good thermal stability and a molecular weight of 49.68 kDa (±4.436%). LP-EPS shows a high antioxidant capacity, anti-inflammatory and anti-sensitizing activity during in vitro experimental studies, with half clearance (IC50) rates of 0.449, 1.314, and 2.369 mg/mL for the ABTS, DPPH, and OH radicals, respectively, and a half inhibition rate (IC50) of hyaluronidase of 1.53 mg/mL. A cell-based assay, enzyme-linked immunosorbent assay (ELISA), and quantitative real-time fluorescence PCR (qRT-PCR) show that LP-EPS effectively treats or ameliorates the skin inflammatory responses triggered by UVB irradiation, as evidenced by a highly significant decrease in the secretion of inflammatory factors by human skin keratinocytes (HaCaT), and a highly significant downregulation of the mRNA expression of MAPK/AP-1 pathway cytokines.

It is well known that exposure to sunlight is the main cause of oxidative stress in human dermal fibroblasts cells. UVA radiation (320-400 nm) can increase the production of reactive oxygen species (ROS) in cells. By reducing the content of ROS, some active enzyme substances with various antioxidant effects can affect oxidative stress. 1 The enzyme antioxidant system mainly includes superoxide dismutase (SOD), catalase (CAT), and glutathione peroxidase

Purpose Damage caused by oxidative stress leads to the premature aging of cells. Mogrosides, the main active components of Siraitia grosvenorii , have strong antioxidant activity; however, it is unclear whether mogroside V (MV) exerts these effects in skin cells. This was investigated in the present study by evaluating the protective effects of MV against oxidative damage induced by hydrogen peroxide (H 2 O 2 ) in skin fibroblasts. Methods Mouse skin fibroblasts (MSFs) were treated with H 2 O 2 and cell viability, total antioxidant capacity, reactive oxygen species (ROS) production, malondialdehyde (MDA) content, and antioxidant enzyme activity were assessed. Results Treatment with MV reduced the ROS level and MDA content in MSFs treated with H 2 O 2 . This was accompanied by increased superoxide dismutase (SOD), glutathione peroxidase (GSH-Px), and catalase (CAT) activities. Conclusion MV reduces H 2 O 2 -induced oxidative stress and enhances endogenous antioxidant activity in skin fibroblasts. Thus, MV can potentially be used as an ingredient in anti-aging cosmetic products.

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