“…This is probably because L. curvatus fermentation breaks down Euryale ferox polysaccharides and polypeptides into lower molecular weight polysaccharides and polypeptides. In our earlier investigation, we confirmed that the molecular weight of Dendrobium officinale polysaccharides was reduced both before and after fermentation [ 26 ]. Changes in the content and compound level structure of bioactive substances during fermentation due to microbial metabolism could explain these findings.…”
This study investigated the effects of Lactobacillus curvatus fermentation on the oxidative stress attenuating effects of Euryale ferox on H2O2-induced human skin fibroblasts (HSF). The results showed that Lactobacillus curvatus fermentation (i) increases the content of the various bioactive components of Euryale ferox and is found to have smaller molecular weights of polysaccharides and polypeptides; (ii) increases the overall intracellular and extracellular antioxidant capacity of H2O2-induced HSF while reducing reactive oxygen species (ROS) levels. Superoxide dismutase (SOD), glutathione peroxidase (GSH-Px), and catalase (CAT) all showed simultaneous increases in activity. Aside from that, the Nrf2 and MAPK signaling pathways are activated to regulate downstream-associated proteins such as the Bax/Bcl-2 protein ratio, matrix metalloproteinase 1 (MMP-1) activity, and human type I collagen (COL-1). These results suggested that the fermentation of Euryale ferox with Lactobacillus curvatus enhances its antioxidant capacity and attenuates apoptosis and senescence caused by oxidative stress.
“…This is probably because L. curvatus fermentation breaks down Euryale ferox polysaccharides and polypeptides into lower molecular weight polysaccharides and polypeptides. In our earlier investigation, we confirmed that the molecular weight of Dendrobium officinale polysaccharides was reduced both before and after fermentation [ 26 ]. Changes in the content and compound level structure of bioactive substances during fermentation due to microbial metabolism could explain these findings.…”
This study investigated the effects of Lactobacillus curvatus fermentation on the oxidative stress attenuating effects of Euryale ferox on H2O2-induced human skin fibroblasts (HSF). The results showed that Lactobacillus curvatus fermentation (i) increases the content of the various bioactive components of Euryale ferox and is found to have smaller molecular weights of polysaccharides and polypeptides; (ii) increases the overall intracellular and extracellular antioxidant capacity of H2O2-induced HSF while reducing reactive oxygen species (ROS) levels. Superoxide dismutase (SOD), glutathione peroxidase (GSH-Px), and catalase (CAT) all showed simultaneous increases in activity. Aside from that, the Nrf2 and MAPK signaling pathways are activated to regulate downstream-associated proteins such as the Bax/Bcl-2 protein ratio, matrix metalloproteinase 1 (MMP-1) activity, and human type I collagen (COL-1). These results suggested that the fermentation of Euryale ferox with Lactobacillus curvatus enhances its antioxidant capacity and attenuates apoptosis and senescence caused by oxidative stress.
“…A UVA-induced fibroblast damage model was then established to study the protective effects of RRT fruit fermentation broth on UVA-induced oxidative stress [ 43 ]. In this study, the ROS level in human embryonic skin (HES) cells after UVA irradiation was first detected, and then the protective ability of RRT fruit fermentation broth on fibroblasts was evaluated by detecting the enzyme activity of intracellular antioxidant enzymes such as CAT, GSH-px, and SOD, as well as the total antioxidant capacity and lipid oxidation level in cells.…”
This study takes the fruit of Rosa roxburghii Tratt (RRT) as a fermentation substrate and carries out a quantitative visual analysis of the domestic and foreign literature on screenings of five different lactic acid bacteria to obtain a fermentation broth. Systemic anti-photoaging effects are analyzed at the biochemical, cellular, and molecular biological levels. DPPH and ABTS free radical scavenging activities are used to verify the antioxidant capacity of the RRT fruit fermentation broth in vitro. Human embryonic skin fibroblasts (HESs) are used to establish a UVA damage model, and the antioxidant capacity of the RRT fruit fermentation broth is verified in terms of intracellular reactive oxygen species (ROS) and antioxidant enzyme activity. RT-qPCR and ELISA are used to detect the expression of TGF-β/Smad, MMPs, and the MAPK/AP-1 and Nrf2/Keap-1 signaling pathways in order to explore the anti-oxidation and anti-photoaging effects of the RRT fruit fermentation broth by regulating different signaling pathways. The results show that an RRT fruit fermentation broth can effectively protect cells from oxidative stress caused by UVA and has significant anti-photoaging effects, with the co-cultured Lactobacillus Yogurt Starter LYS-20297 having the highest overall effect.
“…According to the previous method and further improved, [35] a Tsk gel 2000 SWxl 300 mm × 7.8 mm reversed-phase chromatographic column was used to prepare 5 mg/mL of BSA (Mr = 6,7000), vitamin B12 (Mr = 1,335), and oxidized glutathione The mixed solution of peptide (Mr = 614) was used as the standard to make a standard curve, and the signal was detected at 280 nm. Peptide sequence detection.…”
Section: Methodsmentioning
confidence: 99%
“…Antioxidation. According to our previous method, [35] the Fenton method and DPPH method were used to detect the scavenging ability of YTCY-EPs on hydroxyl radicals and nitrogen free, and a Reactive Oxygen Species Assay Kit (Beyotime, S0033S) was used to detect the ROS content in the cells.…”
As a probiotic, Weizmannia coagulans (W. coagulans) is often used in food and medicine to regulate intestinal ora and resist speci c in ammation. In this study, the anti-acne e cacy and mechanism of YTCY extracellular proteins (YTCY-EPs) from a strain of W. coagulans are analyzed. The main components of YTCY-EPs, extracted and separated from the fermentation broth, are peptides ranging from 1.51-11.44 kDa, accounting for about 80%. Among the peptides identi ed by LC/MS-MS, YTCY A-F possess the properties of antimicrobial peptides, while YTCY 1-4 possess antioxidative properties. These peptides have a strong effect on Cutibacterium acnes (C. acnes) and signi cantly inhibit Staphylococcus aureus. The adhesion of YTCY-EPs has a 50% inhibition rate. It is found that YTCY-EPs possess strong antioxidant and anti-in ammatory properties, and can reduce the downstream TLR2/NF-κB and MAPKs/AP-1 pathways by regulating the nuclear translocation of NF-κB and AP-1 in vitro. The transcriptional expression of in ammatory cytokines, in ammatory chemokines, and matrix metalloproteinase genes is also regulated, thereby slowing the recruitment of in ammatory cells and the development of in ammation, and increasing keratinocyte mobility. YTCY-EPs can also effectively solve such problems as erythema, papules, cysts, skin lesions, hyperkeratinization, and desquamation caused by C. acnes in rabbit ears. Additionally, the treatment effectively improves the condition of wounds and in ammation. The results of this study prove that YTCY-EPs can be used as a potential anti-acne raw material in cosmetics.
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