Physicochemical and Anti-UVB-Induced Skin Inflammatory Properties of Lacticaseibacillus paracasei Subsp. paracasei SS-01 Strain Exopolysaccharide

Abstract

The exopolysaccharide secreted by Lacticaseibacillus paracasei subsp. paracasei SS-01 strain (LP-EPS) is isolated and purified from yogurt. It is a polysaccharide with a branched and multi-stranded structure, which exists in a smooth rod-like or cloud-like state, and possesses a good thermal stability and a molecular weight of 49.68 kDa (±4.436%). LP-EPS shows a high antioxidant capacity, anti-inflammatory and anti-sensitizing activity during in vitro experimental studies, with half clearance (IC50) rates of 0.449, 1.314, and 2.369 mg/mL for the ABTS, DPPH, and OH radicals, respectively, and a half inhibition rate (IC50) of hyaluronidase of 1.53 mg/mL. A cell-based assay, enzyme-linked immunosorbent assay (ELISA), and quantitative real-time fluorescence PCR (qRT-PCR) show that LP-EPS effectively treats or ameliorates the skin inflammatory responses triggered by UVB irradiation, as evidenced by a highly significant decrease in the secretion of inflammatory factors by human skin keratinocytes (HaCaT), and a highly significant downregulation of the mRNA expression of MAPK/AP-1 pathway cytokines.

Isolation and characterization of exopolysaccharide derived from Lacticaseibacillus paracasei AS20(1) with probiotic potential and evaluation of its antibacterial activity

“…Previous studies on EPS of L. paracasei and L. paracasei strains reported its various bioactivities, including antioxidant (Xiao et al 2021), anti-inflammatory, antisensitizing (Su et al 2022), anti-biofilm (Bhat and Bajaj 2019), anti-allergenic (Gibson et al 2017), emulsification and hypocholesterolaemic activities and also prebiotic effect (Bhat and Bajaj 2019). Also, it has been reported that this EPS inhibits the enterobacteria and increases the production of propionic and butyric acid (Bengoa et al 2020).…”

“…2021), anti‐inflammatory, anti‐sensitizing (Su et al . 2022), anti‐biofilm (Bhat and Bajaj 2019), anti‐allergenic (Gibson et al . 2017), emulsification and hypocholesterolaemic activities and also prebiotic effect (Bhat and Bajaj 2019).…”

Two Laminaria japonica Fermentation Broths Alleviate Oxidative Stress and Inflammatory Response Caused by UVB Damage: Photoprotective and Reparative Effects

“…To damage HaCaT cells, 10 mJ/cm 2 UVB was used. References [ 42 , 43 ] performed ROS content determination on damaged HaCaT cells that cultured in a 6-well plate. A ROS Detection Kit was selected for the experiment, the intracellular ROS fluorescence content was observed under a fluorescence inverted microscope (CKX53, Olympus LS, Tokyo, Japan) and the fluorescence intensity was recorded.…”

“…The molecular weights of LJ-Wp, LJ-Gp, and LJ-Yp were determined by GPC-LS-IR, and the morphological characteristics of them were determined using an FEI Nova Nano SEM 450 instrument. The specific experimental method refer to Zhang et al [ 43 ].…”