One hundred and eight strains of Aeromonus from clinical and environmental samples were speciated. Seven species were identified, the most prevalent of which was A. hydrophila. Experimental studies in an animal model with 36 representative strains of different species revealed that all strains could cause significant fluid accumulation in rabbit ileal loops. Of 107 strains showing single or multiple antimicrobial resistance, the highest incidence of resistance was shown for p-lactam antibiotics other than cefotaxime. Transferable resistance plasmids, encoding resistance to ampicillin, cephalexin, cefoxitin, erythromycin and strains. A further markers, including plasmid DNA. The furazolidone, either alone or in combination, were detected in 35 proportion of strains could be cured of one or more resistance resistance to nalidixic acid, and this was accompanied by the loss of plasmids ranged in size between 85.6 and >150 kb.
16S rDNA PCR improves rates of detection of bacteria in pleural fluid, and can detect bacterial species in a single assay as well as identifying unusual and unexpected causal agents.
Complexes of vanillin-4-methyl-4-phenyl-3-thiosemicarbazone with cobalt(II), nickel((II), copper(II) and zinc(II) metal ions were synthesized and characterized by elemental analysis, molar conductance, magnetic moment, infrared spectra, electronic spectra, 1 H and 13 C NMR, mass spectrum and thermal analysis. The low molar conductance values indicate that all the complexes are non-electrolytes. Spectral studies indicate that the ligand act in a tridentate manner coordinating with the metal ion through sulphur, oxygen and nitrogen atoms. Based on magnetic moment measurements, spectral studies and thermal analysis, suitable geometry was proposed for the metal complexes. Thermal stability of the complexes was ascertained on the basis of thermo gravimetric analysis and the lattice parameters, space group and grain size of the ligand and complexes were determined by using powder XRD measurements. Surface morphology of ligand and complexes were studied using SEM analysis. Antimicrobial studies were carried out against gram-positive, gram-negative bacteria and two fungal pathogens.
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