Bacteriophage Therapy of Methicillin Resistant Staphylococcus Aureus Biofilms on Orthopaedic Implants in Rabbit Model

Nath, Gopal

doi:10.24947/baojort/1/1/104

Cited by 2 publications

(5 citation statements)

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“…One liter volume of water was collected in the sterile bottle from one foot below the surface. Further, the samples were immediately transferred to the laboratory and were processed as per the method described by Gupta et al (2016) with slight modifications. Before treatment with chloroform, the samples were centrifuged for 10778 × g for 10 min in a refrigerated centrifuge (Remi CPR-24 plus).…”

Section: Source Sample Collection and Processing Of Water Samplesmentioning

confidence: 99%

“…Bacteriophage isolation was performed using double agar overlay method with slight modification against this strain from different water sources (river, ponds, and sewer) (Gupta et al, 2016). Bacterial lawn culture (10 8 CFU/ml suspensions) was made and was incubated for 2 h. Water specimens from different water bodies were treated with 1% chloroform (v/v) for 20 min and centrifuged for 10 min at 10778 × g, which was dropped over the bacterial lawn and the plates were incubated for overnight at 37˚C.…”

Section: Isolation and Purification Of Specific Bacteriophagesmentioning

confidence: 99%

“…Bacteriophages were isolated as per the standardized protocol described by Nath et al 2019 (Gupta et al, 2016;, while phage counting was done by soft agar overlay method of . The bacteriophages specificity was tested against isolated XDR K. pneumoniae clinical strains using plaque formation by using a soft agar overlay method.…”

Section: Isolation Of Bacteriophagesmentioning

confidence: 99%

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RETRACTED: Study on the Effect of Oral Administration of Bacteriophages in Charles Foster Rats With Special Reference to Immunological and Adverse Effects

et al. 2021

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Numerous pre-clinical and clinical studies have recently demonstrated the significant role of phage therapy in treating multidrug-resistant bacterial infections. However, only a few researchers have focused on monitoring the phage-mediated adverse reactions during phage therapy. Besides adverse reactions, immunological response after short- and long-term oral administration of bacteriophages is also lacking. In this study, we administered the bacteriophages orally against Klebsiella pneumoniae XDR strain in dosages of 1015 PFU/ml and a 1020 PFU/ml (still higher) to Charles Foster rats as a single dose (in acute toxicity study) and daily dosage for 28 days (in sub-acute toxicity study). One milliliter suspension of bacteriophages was administered through the oral gavage feeding tube. No adverse effect was observed in any of the experimental as well as in the control animals.Further, an insignificant change in food and water intake and body weight was observed throughout the study period compared with the control group rats. On the 28th day of phage administration, blood was collected to estimate hematological, biochemical, and cytokines parameters. The data suggested no difference in the hematological, biochemical, and cytokine profile compared to the control group. No significant change in any of the treatment groups could be observed on the gross and histopathological examinations. The cytokines estimated, interleukin-1 beta (IL-1β), IL-4, IL-6, and INF-gamma, were found within the normal range during the experiment. The results suggested no adverse effect, including the severe detrimental impact on oral administration of high (1015 PFU/ml) and very high dose (1020 PFU/ml) of the bacteriophages cocktail. The high and long-term oral administration of bacteriophages did not induce noticeable immunological response as well.

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Section: Source Sample Collection and Processing Of Water Samplesmentioning

confidence: 99%

Section: Isolation and Purification Of Specific Bacteriophagesmentioning

confidence: 99%

Section: Isolation Of Bacteriophagesmentioning

confidence: 99%

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RETRACTED: Study on the Effect of Oral Administration of Bacteriophages in Charles Foster Rats With Special Reference to Immunological and Adverse Effects

et al. 2021

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“…Several preclinical studies have shown the great potential of phages in curing the infection in both acute and chronic infections in animal model. [2][3][4] We have also shown their efficacy against Pseudomonas aeruginosa biofilm in mice model. 5 In the present study, we have evaluated the efficacy of bacteriophages by surface application on chronic wound specifically caused by Staphylococcus aureus, Escherichia coli, and Pseudomonas aeruginosa.…”

Section: Introductionmentioning

confidence: 84%

“…Several preclinical studies have shown the great potential of phages in curing the infection in both acute and chronic infections in animal model. 2-4 We have also shown their efficacy against Pseudomonas aeruginosa biofilm in mice model. 5…”

Section: Introductionmentioning

confidence: 86%

Bacteriophage Therapy of Chronic Nonhealing Wound: Clinical Study

Gupta

Singh

Shukla

et al. 2019

The International Journal of Lower Extremity Wounds

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Background: A chronic wound usually results due to halt in the inflammatory phase of wound healing. Bacterial infections and biofilm formation are considered to be the basic cause of it. Chronic wounds significantly impair the quality of life. Antibiotics are now failing due to biofilm formation emergence of drug-resistant bacteria. Objective: This study aims to see the effect of bacteriophage therapy in chronic nonhealing wound infected with the following bacteria: Escherichia coli, Staphylococcus aureus, and Pseudomonas aeruginosa. Subject: Patients with chronic nonhealing wound not responding to conventional local debridement and antibiotic therapy were included in the study. The age of patients ranged between 12 and 60 years. Method: A total of 20 patients selected and tissue biopsies and wound swabs were taken for isolation of the bacteria. After confirmation of organism, a cocktail of customized bacteriophages was topically applied over the wound on alternate days till the wound surface became microbiologically sterile. Mean bacterial count and clinical assessment were done and compared at the time of presentation and after 3 and 5 doses of application. Results: A significant improvement was observed in the wound healing, and there were no signs of infection clinically and microbiologically after 3 to 5 doses of topical bacteriophage therapy. Seven patients achieved complete healing on day21 during follow up while in others healthy margins and healthy granulation tissue were observed. Conclusion: Topical bacteriophage application may be quite effective therapy for the treatment of chronic nonhealing wounds.

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Bacteriophage Therapy of Methicillin Resistant Staphylococcus Aureus Biofilms on Orthopaedic Implants in Rabbit Model

Cited by 2 publications

References 0 publications

RETRACTED: Study on the Effect of Oral Administration of Bacteriophages in Charles Foster Rats With Special Reference to Immunological and Adverse Effects

RETRACTED: Study on the Effect of Oral Administration of Bacteriophages in Charles Foster Rats With Special Reference to Immunological and Adverse Effects

Bacteriophage Therapy of Chronic Nonhealing Wound: Clinical Study

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