Biochemical characterisation, enteropathogenicity and antimicrobial resistance plasmids of clinical and environmental Aeromonas isolates

Chaudhury, Abhijit; Nath, Gopal; Shukla, B. N.; Sanyal, Sudip

doi:10.1099/00222615-44-6-434

Cited by 23 publications

(18 citation statements)

References 14 publications

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“…DePaola et al (8) found similarly high proportions of OTCresistant aeromonads from catfish and their environments (58 to 83%), where the drug was routinely used in medicated feed. Other comparable investigations of motile aeromonads from different freshwater environments report considerably lower tetracycline resistance levels (7,10,11,23,28,42). One explanation may be that, once acquired, the resistance genes are maintained within the population, protecting the bacteria from tetracyclines produced by other members of the microflora or residues in agricultural or domestic effluents.…”

Section: Discussionmentioning

confidence: 93%

“…Some species, however, have been isolated from local or generalized human infections (7,15,20). By contrast, many members of the group are recognized as primary pathogens to a wide range of cold-blooded animals, in particular to fish (4,6,40).…”

mentioning

confidence: 99%

“…Both types of resistance have been reported to be encoded by transferable plasmids within the genus Aeromonas (1,3,7,14). Several classes of tetracycline resistance determinants have been described on R-plasmids within this group of bacteria (1,2,5,8,9,30).…”

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confidence: 99%

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Incidence, Distribution, and Spread of Tetracycline Resistance Determinants and Integron-Associated Antibiotic Resistance Genes among Motile Aeromonads from a Fish Farming Environment

Schmidt¹,

Bruun²,

Dalsgaard³

et al. 2001

Appl Environ Microbiol

252

192

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A collection of 313 motile aeromonads isolated at Danish rainbow trout farms was analyzed to identify some of the genes involved in high levels of antimicrobial resistance found in a previous field trial (A. S. Schmidt, M. S. Bruun, I. Dalsgaard, K. Pedersen, and J. L. Larsen, Appl. Environ. Microbiol. 66:4908-4915, 2000), the predominant resistance phenotype (37%) being a combined oxytetracycline (OTC) and sulphadiazine/trimethoprim resistance. Combined sulphonamide/trimethoprim resistance (135 isolates) appeared closely related to the presence of a class 1 integron (141 strains). Among the isolates containing integrons, four different combinations of integrated resistance gene cassettes occurred, in all cases including a dihydrofolate reductase gene and a downstream aminoglycoside resistance insert (87 isolates) and occasionally an additional chloramphenicol resistance gene cassette (31 isolates). In addition, 23 isolates had "empty" integrons without inserted gene cassettes. As far as OTC resistance was concerned, only 66 (30%) out of 216 resistant aeromonads could be assigned to resistance determinant class A (19 isolates), D (n ‫؍‬ 6), or E (n ‫؍‬ 39); three isolates contained two tetracycline resistance determinants (AD, AE, and DE). Forty OTC-resistant isolates containing large plasmids were selected as donors in a conjugation assay, 27 of which also contained a class 1 integron. Out of 17 successful R-plasmid transfers to Escherichia coli recipients, the respective integrons were cotransferred along with the tetracycline resistance determinants in 15 matings. Transconjugants were predominantly tetA positive (10 of 17) and contained class 1 integrons with two or more inserted antibiotic resistance genes. While there appeared to be a positive correlation between conjugative R-plasmids and tetA among the OTCresistant aeromonads, tetE and the unclassified OTC resistance genes as well as class 1 integrons were equally distributed among isolates with and without plasmids. These findings indicate the implication of other mechanisms of gene transfer besides plasmid transfer in the dissemination of antibiotic resistance among environmental motile aeromonads.

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Section: Discussionmentioning

confidence: 93%

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confidence: 99%

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Incidence, Distribution, and Spread of Tetracycline Resistance Determinants and Integron-Associated Antibiotic Resistance Genes among Motile Aeromonads from a Fish Farming Environment

Schmidt¹,

Bruun²,

Dalsgaard³

et al. 2001

Appl Environ Microbiol

252

192

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“…Like enteric Gram-negative bacteria, the emergence of resistance among aeromonads will be accelerated by the clinical use of antibiotics (Chaudhury et al, 1996).…”

Section: Resultsmentioning

confidence: 99%

Antibiotic resistance of Aeromonas hydrophila isolated from marketed fish and prawn of South India

Vivekanandhan

Savithamani

Hatha³

et al. 2002

International Journal of Food Microbiology

162

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A total of 319 strains of Aeromonas hydrophila were isolated from 536 fish and 278 prawns for a 2-year period. All the strains were tested for resistance to 15 antibiotics and 100% of the strains was resistant to methicillin and rifampicin followed by bacitracin and novobiocin (99%). Only 3% of the strains exhibited resistance against chloramphenicol. The multiple antibiotic resistance (MAR) indexing of A. hydrophila strains showed that all of them originated from high-risk sources. D

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“…Like Gram negative bacilli, the emergence of resistance among Aeromonads will be accelerated by the extensive clinical use of antibiotics (Ko and Chung, 1995;Chaudhury et al, 1996). Such high level of multiple drug resistance may arise from selective pressure due to the indiscriminate use of antibiotics.…”

Section: Multiple Antibiotic Resistance (Mar) Index Of Aeromonas Hydrmentioning

confidence: 99%

Slime producing, heavy metals and antibiotics resistance in Aeromonas hydrophila isolated in Tunisia

Saidi¹,

Lagha²,

Abdallah³

et al. 2013

Afr. J. Microbiol. Res.

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Aeromonas hydrophila strains isolated from different naturally polluted environments (ten from wastewater, six from bay used for aquaculture, eight from sea coast water and six from fish) were subjected to 13 antibiotics, and to four heavy metals (Copper, Cobalt, Zinc and Mercury) by using agar diffusion and agar dilution methods, respectively. In addition, effect of heavy metals on slime production was also investigated. Results of the antibiotic resistance agreed with those of heavy metals resistance, however, treated wastewater and bay strains were much tolerant than seawater and fish bacteria. The range of metal concentrations that was tolerated in the liquid media yielded information on the tolerance levels of A. hydrophila to different tested concentrations of metals. Copper and zinc were the best tolerated metals. Mercury was the most toxic component for all bacteria. Almost all A. hydrophila produced slime and a small number of strains have changed their morphotype under the heavy metals concentration. Our results have shown that Tunisian aquatic biotopes have a significant proportion of antibiotic and heavy metal resistant to A. hydrophila.

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Biochemical characterisation, enteropathogenicity and antimicrobial resistance plasmids of clinical and environmental Aeromonas isolates

Cited by 23 publications

References 14 publications

Incidence, Distribution, and Spread of Tetracycline Resistance Determinants and Integron-Associated Antibiotic Resistance Genes among Motile Aeromonads from a Fish Farming Environment

Incidence, Distribution, and Spread of Tetracycline Resistance Determinants and Integron-Associated Antibiotic Resistance Genes among Motile Aeromonads from a Fish Farming Environment

Antibiotic resistance of Aeromonas hydrophila isolated from marketed fish and prawn of South India

Slime producing, heavy metals and antibiotics resistance in Aeromonas hydrophila isolated in Tunisia

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