Antibiotic resistance of Aeromonas hydrophila isolated from marketed fish and prawn of South India

Vivekanandhan, G.; Savithamani, K.; Hatha, A.A. Mohamed; Lakshmanaperumalsamy, P.

doi:10.1016/s0168-1605(02)00009-0

Cited by 163 publications

(104 citation statements)

References 16 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…It is considered a serious economic problem, in addition to the fact that led to the indiscriminate use of antibiotics, which has increased resistance of bacteria to antibiotics worldwide (Vivekanandhan et al, 2002;Vieira, 2003).…”

Section: Discussionmentioning

confidence: 99%

Proposed method for agglutinating antibody titer analysis and its use as indicator of acquired immunity in pacu, Piaractus mesopotamicus

et al. 2014

View full text Add to dashboard Cite

Antibody can be assessed by agglutinating antibody titer which is a quantitative measure of circulating antibodies in serum from fish previously immunized. The antibody evaluation has been performed with different fish species, and is considered a reliable method that can be applied to confirm several hypothesis regarding acquired immunity, even in conjunction with precise methods to describe immune mechanisms. In order to provide appropriate analytical methods for future studies on the specific immune system of native fish, the present study standardized on assay to measure the serum agglutinating antibody titer produced after immunization with inactivated A. hydrophila and levamisole administration in pacu. It was possible to determine the agglutinating antibodies titer in a satisfactorily way in pacu immunized with inactive A. hydrophila, and the highest titers were observed on fish fed with levamisole.Keywords: fish, methodology, immunology, acquire immunology, humoral immunity. Aglutinante de anticorpos como indicador de imunidade adquirida de pacu Piaractus mesopotamicus ResumoOs anticorpos podem ser avaliados pelo título aglutinante de anticorpos, que é uma medida quantitativa de anticorpos no soro de peixe previamente imunizados. A determinação do título de anticorpos foi realizada com diversas espécies de peixes e é considerado um método confiável que pode ser aplicado para confirmar diversas hipóteses que envolvam o sistema adquirido de defesa, mesmo em conjunto com métodos precisos, para descrever mecanismos imunes. A fim de prover métodos analíticos adequados para futuros estudos sobre o sistema imune específico de peixes nativos, o presente estudo aperfeiçoou o ensaio para avaliar o título aglutinante de anticorpos em soro de pacu imunizados com A. hydrophila e alimentados com levamisol. Foi possível determinar o título aglutinante de anticorpos de forma satisfatória, em pacus imunizados com A. hydrophila inativa, e os maiores títulos foram observados em peixes alimentados com levamisol.Palavras-chave: peixe, metodologia, imunologia, imunidade adquirida, imunidade humoral.

show abstract

Section: Discussionmentioning

confidence: 99%

Proposed method for agglutinating antibody titer analysis and its use as indicator of acquired immunity in pacu, Piaractus mesopotamicus

et al. 2014

View full text Add to dashboard Cite

show abstract

“…Multiple antibiotic resistance (MAR) has been registered for A. hydrophila isolated from freshwater fish farms in association with a variety of drugs, commonly used as feed additives (Aoki et al 1971;Pettibone et al 1996;Son et al 1997;Vivekanandhan et al 2002). The main problem involving the use of antibiotics against aeromonas infections is the development of resistance by these bacteria (Aoki et al 1971), generally related to the presence of plasmids (Ansary et al 1992).…”

Section: Introductionmentioning

confidence: 99%

Prevalence and antibiotic susceptibility of Aeromonas hydrophila isolated from freshwater fishes

Sarder¹,

Khan²,

Saha³

et al. 2016

J. Fish.

View full text Add to dashboard Cite

CitationSarder H, Khan T, Saha ML, Punom NJ, Mandal SC and Rahman MS (2016) Prevalence and antibiotic susceptibility of Aeromonas hydrophila isolated from freshwater fishes. Journal of Fisheries 4(3): 411-419. DOI: 10.17017/jfish.v4i3.2016.177 Abstract Aeromonas hydrophila is an opportunistic microorganism. It is a secondary biological agent that contributes to the occurrence of fish diseases and its deterioration. This research was undertaken to determine the prevalence of A. hydrophila in some freshwater fishes collected from three different fish markets of Dhaka City and to test their antibiotic susceptibility. Total bacterial count and total aeromonas on different aeromonas selective media were enumerated using serial dilution technique. Bacterial isolates were characterized to identify A. hydrophila using biochemical tests and with comparison to reference strain (ATCC 7966). The lowest Aeromonas count was detected to be 2.83±0.40×10 2 cfu/g in Anabas testudineus and the highest was 1.03±0.153×10 3 cfu/g in Oreochromis mossambicus. On market basis highest aeromonas count was found in Anando Bazar (8.10±1.09×10 2 cfu/g) and lowest in Hatirpool Bazar (5.63±0.90×10 2 cfu/g) with no significant difference. Maximum susceptibility to amikacin and gentamicin was observed whereas all of the isolates were found resistant to a commonly used antibiotic amoxycillin. The obtained results point that antimicrobial susceptibility was more or less similar regardless of the origin of the samples collected. All the fishes investigated in this study contained A. hydrophila in their different organs.

show abstract

“…Infectious diseases caused by these pathogens result in more than 85% mortality in fingerling and yearling grass carp, leading to huge economic losses (Jiang, 2009;Jang et al, 2010). Preventing these infectious diseases is still a great challenge because of environmental concerns associated with chemical treatments and the emergence of multiple resistances to antibiotics (Vivekanandhan et al, 2002). Thus, extensive research on the molecular genetics of grass carp and a deeper understanding of their immune defense mechanisms are required to develop more efficient management strategies for disease control.…”

Section: Introductionmentioning

confidence: 99%

Differential expression of interleukin-12 p35 and p40 subunits in response to Aeromonas hydrophila and Aquareovirus infection in grass carp, Ctenopharyngodon idella

Pandit¹,

Shen²,

Xu³

et al. 2015

Genet. Mol. Res.

View full text Add to dashboard Cite

ABSTRACT. The grass carp (Ctenopharyngodon idella) aquaculture industry in Asia is prone to bacterial and viral hemorrhagic diseases. Effective adjuvants for vaccine formulation are the need of the hour for control of these diseases and long-term sustainability of grass carp farming. In this study, the involvement of interleukin-12 (IL-12) from grass carp (gcIL-12) in anti-bacterial and anti-viral immune responses was demonstrated via expression profiles of gcIL-12 subunits in immune tissues of the fish, following infection by Aeromonas hydrophila and Aquareovirus. Additionally, cDNA of the gcIL-12 subunits, p35 and p40 was cloned and characterized. We found that most of the structurally and functionally important features of vertebrate orthologues were conserved in gcIL-12 subunits, p35 and p40, with some features specific to grass carp. High levels of gcIL-12 p35 expression in the brain and gills suggest that IL-12 plays an important role in neural and immune systems. High expression levels in the heart, blood, and immune-related tissues suggest an important role in circulation and the immune system as well. Infections by both, A. hydrophila and Aquareovirus stimulated the mRNA expression of gcIL-12 subunits, p35 and p40 in most immune tissues. Significant upregulation or downregulation of gcIL-12 subunits, p35 and p40 by bacterial and viral infection confirms their potential role in anti-bacterial and anti-viral immune responses in fish.

show abstract

Antibiotic resistance of Aeromonas hydrophila isolated from marketed fish and prawn of South India

Cited by 163 publications

References 16 publications

Proposed method for agglutinating antibody titer analysis and its use as indicator of acquired immunity in pacu, Piaractus mesopotamicus

Proposed method for agglutinating antibody titer analysis and its use as indicator of acquired immunity in pacu, Piaractus mesopotamicus

Prevalence and antibiotic susceptibility of Aeromonas hydrophila isolated from freshwater fishes

Differential expression of interleukin-12 p35 and p40 subunits in response to Aeromonas hydrophila and Aquareovirus infection in grass carp, Ctenopharyngodon idella

Contact Info

Product

Resources

About