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Perimenopausal disorders are among the serious problems affecting the medico biological, psychoso cial, and socio cultural spheres in the lives of the women who entered the climacteric period (peri menopause or climacterium) [9]. Today's women spend a third of their lives in the period of perimeno pause, and, therefore, the quality of life for the women in this age category is now under more careful exami nation [12]. First of all, this is relevant to the diagnos tics, prophylaxis, and correction of the disorders man ifested by psychoemotional and neurovegetative symptoms making up the climacteric syndrome (CS). Clinico epidemiological studies on the frequency rate for CS and premenopausal dysfunctional uterine bleedings give evidence for its present growth to 50-65% [1, 3, 4, 10]. The CS associated pathological manifestations disturb women's professional life, decrease their social activity, and bring changes to their interpersonal relationships [7,11].Despite a long history of studies on the CS patho genesis and its clinical manifestations, the qualifica tion of perimenopausal disorders still contains a lot of disputable and unsolved questions [8, 10, 13]. As before, the assessment of many significant factors that can affect the development of perimenopausal disor ders is not included. It relates, in the first place, to typological and personal specific characteristics, including type of nervous system, and effects of psy chological and social factors. In other words, a biopsy chosocial approach has not been applied so far to the problem of perimenopausal disorders. MATERIALS AND METHODSThe analytical material taken for our study was based on the results of investigations done in over 500 perimenopausal female subjects. Clinical, psy chological, and social indicators were studied within a biopsychosocial approach. The basic group was com posed of 416 investigated subjects aged from 42 to 55 years with perimenopausal disorders (residents of St. Petersburg and Leningrad oblast). The control group included 138 women from the same region with a norm corresponding course of their perimenopausal period. The two participant groups were comparable by their obstetrical-gynecological anamneses, diag nosed extragenital pathologies, and other epidemio logical characteristics.The following research methods were used: anam nestic; clinical, including observation, the modified menopausal index test (MMI) [16], and the vegetative nervous system status assessment [6]; experimental psychology, including the four dimensional symp tomatic neuroticism scale questionnaire by Alexand rowicz for the diagnostics of neurosis like disorders and impaired psychic adaptation, tests for assessing fears and personal anxiety by the Ch.D. SpielbergerYu.L. Hanin anxiety scale, tests for assessing the emo Abstract-Perimenopausal disorders belong to serious problems involving the medico biological, psychoso cial, and socio cultural aspects of the lives of the women who entered the perimenopausal period (climacte rium). A contingent of perimenopaus...

We studied the effects of synthesized tripeptides T-32 (Glu-Asp-Ala), T-33 (Glu-Asp-Arg), T-34 (Glu-Asp-Gly), T-36 (Glu-Asp-Pro), and T-38 (Lys-Glu-Asp) on organotypic skin cultures of young and old rats. In skin explants from young rats, all peptides except T-34 produced a stimulating effect on cell proliferation. In skin explants from old rats, tripeptide T-38 produced a marked stimulatory effect on proliferation. Immunocytochemical study of the proapoptotic p53 protein expression showed that cell proliferation increased due to less pronounced apoptosis. The capacity of the studied tripeptides to promote cell proliferation in the skin tissues of young and old animals provides the basis for further study of these substances as preparations boosting the regenerative processes in the skin, including those at age-associated pathology.

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Замедление инволютивных процессов в коже является актуальной задачей геронтокосметологии. В статье рассматриваются пептиды и аминокислоты, регулирующие функциональную активность клеток кожи. Методом иммуноцитохимии изучено влияние аминокислот лизина (Lys), глутаминовой кислоты (Glu), аспарагиновой кислоты (Asp), пептида KED (Lys-Glu-Asp) и полипептидного комплекса сосудов на экспрессию проапоптотических белков р53, р21, р16, р65 в органотипической культуре кожи крыс. Полипептидный комплекс сосудов и пептид KED оказывали более сильный антиапоптотический эффект на клетки кожи по сравнению с L-аминокислотами. Полипептидный комплекс сосудов и пептид KED соответственно на 39-69% и на 40-65% снижали экспрессию белков р53, р21, р16, р65 в культуре клеток кожи, т.е. их влияние на клетки кожи было сопоставимо. Можно полагать, действие полипептидного комплекса сосудов на клетки кожи достигается за счет пептида KED в его составе. Уменьшение экспрессии проапоптотических протеинов может указывать на способность пептида KED и полипептидного комплекса сосудов поддерживать функциональную активность фибробластов кожи при старении. Полученные данные указывают на перспективность дальнейшего изучения полипептидного комплекса сосудов и пептида KED в качестве геропротекторов, поддерживающих функциональную активность клеток кожи. Ключевые слова: апоптоз, кожа, культура клеток, пептид KED, полипептидный комплекс сосудов.

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