Effect of bioregulatory tripeptides on the culture of skin cells from young and old rats

Abstract

We studied the effects of synthesized tripeptides T-32 (Glu-Asp-Ala), T-33 (Glu-Asp-Arg), T-34 (Glu-Asp-Gly), T-36 (Glu-Asp-Pro), and T-38 (Lys-Glu-Asp) on organotypic skin cultures of young and old rats. In skin explants from young rats, all peptides except T-34 produced a stimulating effect on cell proliferation. In skin explants from old rats, tripeptide T-38 produced a marked stimulatory effect on proliferation. Immunocytochemical study of the proapoptotic p53 protein expression showed that cell proliferation increased due to less pronounced apoptosis. The capacity of the studied tripeptides to promote cell proliferation in the skin tissues of young and old animals provides the basis for further study of these substances as preparations boosting the regenerative processes in the skin, including those at age-associated pathology.

A Newly Improved Method of Primary Cell Culture: Tissue Block with Continuous Adhesion Subculture in Skin Fibroblast

“…Previously, the tissue block was often cut into about 1 × 1mm 2 in size with neat and smooth edges (Proudfoot and Shanahan 2011; Voicekhovskaya et al 2012;Mutlu et al 2020). Nevertheless, we, after many pre-experiments, found that 2×2mm 2 size of tissue block was optimum because the too-small tissue block tended to shrink during the adherent process and be unfavorable to reuse, whereas the toolarge one kept the cells poor in nutrients and easy to die.…”