Abstract:Background
Fibroblasts (FBs) have been widely used as a typical in vitro cell model for investigating the biological processes and cell pathophysiological mechanisms. However, FBs are prone to senescence in cell culture process after several passages. Thus, a new approach to cell culture is quite required to enhance the viability of cells.
Objective
To explore a novel method of primary cell culture based on skin FBs.
Methods
Dermal tissue blocks were obtained from BALB/c neonatal mice and randomly divided i… Show more
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