No abstract

No abstract

No abstract

No abstract

A B S T R A C T Nephrogenous cyclic AMP (NcAMP), total cyclic AMP excretion (UcAMP), and plasma immunoreactive parathyroid hormone (iPTH), determined with a multivalent antiserum, were prospectively measured in 55 control subjects, 57 patients with primary hyperparathyroidism (10 HPT), and 10 patients with chronic hypoparathyroidism.In the group with 1°HPT, NcAMP was elevated in 52 patients (91%), and similar elevations were noted in subgroups of 26 patients with mild (serum calcium c 10.7 mg/dl) or intermittent hypercalcemia, 19 patients with mild renal insufficiency (mean glomerular filtration rate, 64 mllmin), and 10 patients with moderate renal insufficiency (mean glomerular filtration rate, 43 ml/min). Plasma iPTH was increased in 41 patients (73%).The development of a parametric expression for UcAMP was found to be critically important in the clinical interpretation of results for total cAMP excretion. Because of renal impairment in a large number of patients, the absolute excretion rate of cAMP correlated poorly with the hyperparathyroid state. Expressed as a function of creatinine excretion, UcAMP was elevated in 81% of patients with 10 HPT, but the nonparametric nature of the expression led to a number of interpretive difficulties. The expression of cAMP excretion as a function of glomerular filtration rate was developed on the basis of the unique features of cAMP clearance in man, and this expression, which provided elevated values in 51 (89%) ofthe patients with 10 HPT, avoided entirely the inadequacies of alternative expressions.Results for NcAMP and UcAMP in nonazotemic and azotemic patients with hypoparathyroidism confirmed An abstract ofthis work appeared in 1976. Clin. Res. 24: 456A.

No abstract

No abstract

Patients with adrenal cortical insufficiency exhibit an increased taste sensitivity for sodium chloride (NaCl) (1). Treatment with carbohydrate-active steroids returns the abnormal threshold to normal levels (1). In a variety of acute and chronic debilitating diseases, a number of them exhibiting major abnormalities of water and electrolyte metabolism, taste threshold for NaCi is comparable to that observed in healthy subjects (1). Taste thresholds for all modalities are decreased in cystic fibrosis, but they do not change after treatment with steroids (2).The present study was designed to examine in detail taste thresholds in adrenal insufficiency and to explore the effect of adrenal cortical hormones on taste. METHODSThe subjects of this study were 13 normal volunteers, aged 6 to 35, two patients with anterior pituitary insufficiency, aged 21 and 36, and seven patients with Addison's disease, aged 25 to 65. All patients with Addison's disease had clinical features of this disease, urinary 17-hydroxycorticosteroids that were below 2 mg per 24 hours and did not increase with adrenocorticotropin (ACTH), 40 U given intravenously over 8 hours, each day for 5 days. Both patients with anterior pituitary insufficiency had hypothyroidism, hypogonadism, and adrenal insufficiency, with urinary 1 7-hydroxycorticosteroids in J.M. and S.P. of 1.1 and 2.2 mg per day, respectively, rising to 27 and 10.9 mg per day, respectively, with ACTH. All patients remained on an air-conditioned metabolic ward and ate a normal diet, which was well tolerated even when they were not receiving treatment. Sodium intake was 100 to 200 mEq per day. Body weight, determined with metabolic scales daily on arising, was used to provide a gross estimate of changes in the volume of body fluids. The patients were studied under each of three conditions: a) untreated for 4 or more days, b) treated with desoxycorticosterone acetate (DOCA), 20 mg per day for 1 to 7 days, and c) treated with prednisolone (A1F), 20 mg per day for 2 to 5 days.In addition, detection thresholds for the taste of NaCl were measured between 7 and 8 a.m. in three normal Caucasian males, aged 18 to 20, before and during treatment with Pitressin. The two levels of sodium intake during Pitressin were 9 mEq and 150 mEq per day. Taste was evaluated by measuring the detection threshold. A detection threshold is defined as the lowest concentration of test solution that can be consistently distinguished from distilled water. Thresholds were measured by one observer in the early morning hours in the normal subjects and in the morning or evening hours, or both, in the patients with adrenal insufficiency. Occasional retesting of the patient by another observer gave comparable results on each occasion. At the time of testing, the patients had not smoked or eaten for at least 1 hour. After the mouth was rinsed with distilled water, the solutions of test substance or glass-distilled water were administered as single drops and placed on alternate sides of the anterior one-third of the tongue.Each...