Objective. To ascertain the essential oil phytochemicals of the leaf and to test for the antibacterial and antioxidant properties of dichloromethane crude extract of Barringtonia asiatica leaf. Methods. The phytochemical screening of essential oils, extraction by hydrodistillation using the Clevenger apparatus, and analysis performed by gas chromatography equipped with a flame ionization detector (GC-FID). Antibacterial activity and the inhibition rate (mm) were determined using the agar disc method against four bacterial strains using tetracycline as positive control. The antioxidant potential of dichloromethane crude extract was investigated spectrophotometrically using 1,1-diphenyl-2-picrylhydrazyl. Results. The essential oil properties were reasonable with major phytochemicals like uncineol 30.9%, eicosane 27.4%, eicosane 21.6%, and 4-propyl-guaiacol 14.05%. The antibacterial activity of the dichloromethane crude extract showed broad-spectrum activity against Salmonella typhi, Escherichia coli, Staphylococcus aureus, and Klebsiella pneumoniae with inhibition value ranges between 2.50 ± 0.10 mm and 5.00 ± 0.06 mm. The dichloromethane crude extract exhibited strong antioxidant activities when compared to the standard. Conclusions. These results suggest that the leaves of Barringtonia asiatica is composed of essential compound as well as antibacterial and antioxidant properties from the crude extract; these are possible due to the presence of some bioactive compounds in the crude extract. The species also showed a reasonable amount of natural products in the essential oils from the hydrodistillation which can as well be used in the cosmetics and food industries.
Objective: The study presented here was carried out for the evaluation of bacterial and cytotoxic potential of chloroform extract of Leaves of Leptadenia hastata. The Bacterial effects was tested against 4 bacteria namely Salmonella typhi, Escherichia coli, Staphylococcus aureus, and Klebsielia pneumonia. Methods:The bacterial effect of the plant Leptadenia hastata was evaluated for potential antimicrobial properties in vitro by agar well diffusion method with four standard bacterial strains. Artemia salina; a brine shrimp species; was used to asses' cytotoxic activity of the plant extract.Result: Among the subjected four bacteria; in case of Staphylococcus aureus, E. coli and Salmonella typhi; their zone of inhibition was higher (1.13 ± 0.15, 1.23 ± 0.12 and 1.03 ± 0.06) at 1000 ppm concentration. Whereas in the case of Klebsielia pneumonia, the zone of inhibition was higher (1.13 ± 0.06) at 500 ppm. The extract exhibited effectiveness against the four species of bacteria was significant. Cytotoxicity property which was reflected in LC50 value and was found to be dose dependent manner Conclusion:The present results showed potential of the medicinal plant used by traditional herbal medical practitioners as natural anti-bacteria and can effectively be used for its significant action. It is also an effective cytotoxic agent and thus justify its use among herbal medical practitioners.
Objective: This study was carried out with an objective to investigate the antibacterial potential of five leaf extract of Leptadenia hastata plant on some bacteria. Methods:Leptadenia hastata extracts were evaluated for potential antimicrobial properties. The leaves of the plant were extracted with n-hexane, dichloromethane, ethyl acetate, chloroform and methanol and then vaporized to give respective extracts. Antimicrobial activity against Escherichia coli, salmonella typhi, staphylococcus aureus and Klebsielia pneumonia, was determined by an agar well diffusion method. The optical density of the broth using UV mini spectrophotometer and zone of inhibition by the crude extract were determined. Results:The results showed that of n-hexane, ethyl acetate, dichloromethane and methanol extracts of Leptadenia hastata, methanolic and chloroform extracts displayed more activity with 1.10±0.10 mm ab and 0.97±0.06 mm ab where a Significantly (p<0.05) higher compared to different extract at the same concentration b Significantly (p<0.05) lower compared to the control, than others at 25-1000 ppm per well of the extracts tested. Conclusion:The present results showed the potential of the medicinal plant used by traditional herbal medical practitioners as natural antimicrobial agents, thus can be further used to determine the bioactive products that may provide as leads in the development of new drugs.
The research work is all about drug discovering in plants through isolation and characterization in the cause of looking an agent for infectious and disease control.
The objective of this study was to evaluate if the plant extract could provide antifungal potential against some pathogenic fungi. Extract of Dichloromethane solvents was used for disc diffusion assay. The inhibitory concentration of the extract was performed by broth dilution method and zone of inhibition was studied by disc diffusion method at the concentration of 25, 50, 100, 250, 500 and 1000ppm in DMSO. Fluconazole was used as the reference control for antifungal study. The extract showed maximum inhibition potential of zone of inhibition against most of the pathogen (Aspergillus niger, Aspergillus flavus, Candida tropicalis and Fusarium oxysporium) used at concentration 25ppm to 250ppm with zone of inhibition (3.45±0, 3.33±0.12, 3.07±0.05 and 2.97±0.10mm respectively). The extract showed minimum inhibition potential against Fusarium oxysporium in all the concentration when compared with the control as well as to the other pathogens. Aspergillus niger and Aspergillus flavus was found to be more sensitive to Dichloromethane leaf extract followed by Candida tropical and lastly fusarium oxyspurium. The Present study indicates the potential usefulness of Dichloromethane leaf extract of Leptadenia hastata as antifungal agent.
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