Antibacterial and Cytotoxic Actions of Chloroform Crude Extract of Leptadenia hastata (Pers) Decnee

Umaru, Isaac John; Badruddin, Fasihuddin A.; Assim, Zaini; Umaru, Hauwa A.

doi:10.4172/2471-2663.1000139

Cited by 11 publications

(14 citation statements)

References 14 publications

(10 reference statements)

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“…The issue of antibacterial resistance emanating has led to the search for new antimicrobial agents. Due to the problem of microbial resistance to antibiotics, attention is given toward biologically active components isolated from plant species commonly used as herbal medicine, as they may offer a new source of antimicrobial activities [14]. Our search for antioxidant and antibacterial activity from this plant Leptadenia hastata ethyl acetate extract revealed an antioxidant and antibacterial activity.…”

Section: Resultsmentioning

confidence: 99%

“…Preparation of agar plates was performed based on method described by Pundir & Jain [15] as reported by Umaru IJ, et al [14]. Nutrient agar was prepared according to manufacturer's instruction with 14 g of dried agar dissolved in 500mL distilled water.…”

Section: Preparation Of Agar Platesmentioning

confidence: 99%

“…Inoculation of the bacteria was carried out in a biohazard cabinet and the procedure was based on method described by Umaru IJ, et al [14]. Approximately 1mL of the ready bacterial broth was transferred into mini centrifuge tubes.…”

Section: Plate Inoculationmentioning

confidence: 99%

“…The leaves are up to 10cm long, mostly ovate and light green. The flowers are cream or yellowish green [7]. Leptadenia hastata is typically grown in tropical dry lands in sandy soil.…”

Section: Introductionmentioning

confidence: 99%

“…Most important effects of these plants are because of compounds, such as alkaloids, tannins, flavonoids, terpenoids, saponins, and phenolic compounds. The plants are now in great demand in the developing world for primary health care not because they are inexpensive but because of their therapeutic, performance, better compatibility and acceptability with human body with low toxicity and minimal side effects [14]. The aim of the study was to evaluate antioxidant and biological activity of the extract obtained from the ethyl acetate extract of the roots of Leptadenia Hastata (Pers.)…”

Section: Introductionmentioning

confidence: 99%

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Antioxidant and Biological Activities of Leptadenia Hastataethyl Acetate Roots Extracts

U¹

2018

MACIJ

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Background: Leptadenia hastata (Pers) Decne is one of the most important medicinal plants in West African traditional medicine for the treatment of inflammation, Ant gonorrhea, stomach ache, hypertension, catarrh, skin diseases, trypanosomiasis and Wound healing. Much attention has been paid to this medicinal plant because of its traditional uses. The aim of the study was to evaluate antioxidant and biological activity of the extract obtained from the ethyl acetate extract of the roots of Leptadenia Hastata (Pers.) Decne. Material and Method: Antioxidant activity of extracts was evaluated by DPPH and the bacterial effect of the plant Leptadenia hastata was evaluated for antimicrobial activity in vitro by agar desk diffusion method with four standard bacterial strains (Staphylococcus aureus, Escherichia coli, Salmonella typhi, Kleibsilia pneumonia). Result: The ethyl acetate possessed strong radical scavenging activity (IC501.889). The extracts were also effective against all Gram-positive and Gram negative bacteria tested, especially for Staphylococcus aureus and Escherichia coli with an inhibition potential of 1.03 ± 0.12 and 1.30 ± 0.10 respectively at 500ppm. Conclusion: These findings give a scientific basis to the traditional usage of Leptadenia hastata (Pers) Decne, also showing its potential as rich sources of natural antioxidant compounds. Thus is a potential candidate to be used as an antioxidant agent, for treatment of other ailment as well as infectious diseases.

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Section: Resultsmentioning

confidence: 99%

Section: Preparation Of Agar Platesmentioning

confidence: 99%

Section: Plate Inoculationmentioning

confidence: 99%

“…The leaves are up to 10cm long, mostly ovate and light green. The flowers are cream or yellowish green [7]. Leptadenia hastata is typically grown in tropical dry lands in sandy soil.…”

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Antioxidant and Biological Activities of Leptadenia Hastataethyl Acetate Roots Extracts

U¹

2018

MACIJ

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Biosynthesis, characterization, and antibacterial activity of ZnO nanoaggregates using aqueous extract from Anacardium occidentale leaf: comparative study of different precursors

Droepenu

Asare

Wee

et al. 2021

Beni-Suef Univ J Basic Appl Sci

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Background Various parts of Anacardium occidentale plant possess curative qualities like antidiabetic, anti-inflammatory, antibacterial, antifungal, and antioxidant. Aqueous extract of this plant leaf was used in biosynthesizing zinc oxide (ZnO) nanoaggregates using two precursors of zinc salt (zinc acetate dihydrate [Zn(CH3COO)2∙2H2O] and zinc chloride [ZnCl2]). The synthesized ZnO samples were used in a comparative study to investigate the antibacterial activity against selected Gram-positive and Gram-negative microbes [Staphylococcus aureus, Exiguobacterium aquaticum (Gram +ve) and Escherichia coli, Klebsiella pneumoniae, Acinetobacter baumannii (Gram −ve)]. The synthesized ZnO nanoaggregates from the two precursors were characterized using Fourier transform infrared spectroscopy (FT-IR), scanning electron microscopy (SEM), transmission electron microscopy (TEM), and energy-dispersive x-ray spectroscopy (EDX) techniques. Results Micrographs of SEM and TEM confirmed nanoparticles agglomerated into aggregates. While spherical nanoaggregates were identified in samples prepared from Zn(CH3COO)2∙2H2O, flake-like structures were identified in samples synthesized from ZnCl2. Particle size determined by TEM was 107.03 ± 1.54 nm and 206.58 ± 1.86 nm for zinc acetate dihydrate and zinc chloride precursors respectively. ZnO nanoaggregate synthesized using zinc acetate as precursor gave higher antibacterial activity than its counterpart, zinc chloride with K. pneumonia recording the highest inhibition zone of 2.08 ± 0.03 mm (67.53%) whereas S. aureus recorded the least inhibition zone of 1.06 ± 0.14 mm (34.75%) for ZnO nanoaggregate from zinc chloride precursor. Also, antibacterial activity increases with increasing concentration of the extract in general. However, A. baumannii, E. aquaticum, and K. pneumoniae did not follow the continuity trend with regards to the 250 ppm and 500 ppm concentrations. Conclusion Biosynthesis of ZnO nanoaggregates using aqueous extract of A. occidentale leaf from zinc acetate dihydrate and zinc chloride as precursors was successful with the formation of nanospheres and nanoflakes. The study suggested that A. occidentale sp. could be an alternative source for the production of ZnO nanoparticles and are efficient antibacterial compounds against both Gram +ve and Gram −ve microbes with its promising effect against infectious bacteria.

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Determination of Antibiotic Susceptibility Pattern of Clinical Isolates of Salmonella Typhi and Escherichia coli

Ali

2018

ARR

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The aim of the study is to determine the antibiotic sensitivity pattern of clinical isolates of Salmonella typhi and Escherichia coli. The isolates were obtained from stool sample of typhoid fever patients attending Muhammad Abdullahi wase Specialist Hospital Kano. Identification of the bacterial isolates was done using conventional standard laboratory methods including Gram staining, cultural characterization and biochemical test. The sensitivity pattern of Salmonella typhi and Escherichia coli was tested against commercially prepared antibiotics sensitivity disc using Kirby-Bauer method. Based on the finding of this study, most of the antibiotics are active against the isolates. Salmonella typhi was susceptible to Travid, Reflacin, Ciprofloxacin, Streptomycin and Gentamicin but resistance to Ampicillin. However, its sensitivity to Septrin Augmentin, Ceporex and Nalidixic acid is intermediate. On the other hand, Escherichia coli were found resistant to Augmentin, Ceporex and Ampicillin while sensitive to Reflacin, Taravid, Septri and Streptomycin while Escherichia coli were found intermediate sensitive to Ciprofloxacin and Gentamicin. Statistical analysis of the result showed that there is no significant different on the susceptibility of the organism to the antibiotics used at p<0.05.

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Antibacterial and Cytotoxic Actions of Chloroform Crude Extract of Leptadenia hastata (Pers) Decnee

Cited by 11 publications

References 14 publications

Antioxidant and Biological Activities of Leptadenia Hastataethyl Acetate Roots Extracts

Antioxidant and Biological Activities of Leptadenia Hastataethyl Acetate Roots Extracts

Biosynthesis, characterization, and antibacterial activity of ZnO nanoaggregates using aqueous extract from Anacardium occidentale leaf: comparative study of different precursors

Determination of Antibiotic Susceptibility Pattern of Clinical Isolates of Salmonella Typhi and Escherichia coli

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