Determination of Antibiotic Susceptibility Pattern of Clinical Isolates of Salmonella Typhi and Escherichia coli

Abstract

The aim of the study is to determine the antibiotic sensitivity pattern of clinical isolates of Salmonella typhi and Escherichia coli. The isolates were obtained from stool sample of typhoid fever patients attending Muhammad Abdullahi wase Specialist Hospital Kano. Identification of the bacterial isolates was done using conventional standard laboratory methods including Gram staining, cultural characterization and biochemical test. The sensitivity pattern of Salmonella typhi and Escherichia coli was tested against commercially prepared antibiotics sensitivity disc using Kirby-Bauer method. Based on the finding of this study, most of the antibiotics are active against the isolates. Salmonella typhi was susceptible to Travid, Reflacin, Ciprofloxacin, Streptomycin and Gentamicin but resistance to Ampicillin. However, its sensitivity to Septrin Augmentin, Ceporex and Nalidixic acid is intermediate. On the other hand, Escherichia coli were found resistant to Augmentin, Ceporex and Ampicillin while sensitive to Reflacin, Taravid, Septri and Streptomycin while Escherichia coli were found intermediate sensitive to Ciprofloxacin and Gentamicin. Statistical analysis of the result showed that there is no significant different on the susceptibility of the organism to the antibiotics used at p<0.05.