ABSTRACT… Objectives:To determine the effects of Allium sativum essential oil (ASEO) phytotherapy on serum triglycerides, total cholesterol, HDLc, LDLc and blood cell counts in albino rat model. Study design: Experimental study. Setting and Duration: Animal House, Sindh Agriculture University and Isra University Hyderabad from May 2014 to January 2015. Materials and Methods: 60 albino rats were divided into four groups. Controls were given Placebo. Experimental rat groups were given ASEO 100 mg/kg, 200 mg/kg and 300 mg/kg orally for 30 days. Cardiac puncture was performed for blood sampling. Research variables were analyzed on Statistix 10.0 (USA). Results: Blood lipids showed significant reduction in various blood lipid fractions. Serum LDLc exhibited with a concomitant rise in serum HDLc (p =0.0001) in high ASEO treated rats. Red blood cells, white blood cells and platelet showed significant improvement ASEO fed rats (p=0.001). Conclusion: Allium sativum essential oil (ASEO) phytotherapy showed a rise in HDLc and a reduction in LDLc, triglycerides and total cholesterol with improvement in red blood cell counts.
Key words:Allium sativum essential oil Blood lipids LDLc HDLc Blood cell counts Rats. Article Citation: Irshad F, Mawani H, Naz S. Allium sativum essential oil (ASEO); effect of supplementation on serum triglycerides, total cholesterol, HDLC, LDLC and blood cell counts in albino rats. Professional Med J 2017;24(4):612-616.
… Objectives: To analyze the hepatoprotective effects of Curcuma longa (CL) against carbon tetrachloride (CCl 4 ) induced chemical injury in experimental rats. Study Design: Experimental study. Setting: Indus Medical College in collaboration with Animal house of Sindh Agriculture University Tando Jam. Period: March 2016 to August 2016. Methodology: Sixty adult male rats were selected according to inclusion and exclusion criteria. Rats were divided randomly into 3 groups -as group A. controls, group B -received CCl 4 and group C-received CCl 4 + CL orally. Blood samples were taken after 4 weeks of therapy by cardiac puncture. 5μ thick liver tissue sections were stained for light microscopy examination. Analysis of data was performed on Statistix 9.0 (USA) at statistical significance of p-value ≤ 0.5. Results: Liver cell biochemical markers of injury and histopathological examination show the Curcuma longa is effective against carbon tetrachloride induced liver injury (P <0.05). Liver histology was improved by the curcuma longa therapy. Conclusion: Liver aminotransferase and histology were improved significantly by the curcuma longa therapy in carbon tetrachloride induced liver injury.
Objectives: Evaluate the mitigating effect of aqueous extract of Ginkgobiloba (GkbE) on liver enzymes and histology in carbon tetrachloride (CCl4) induced liverinjury in albino rat. Study design: Experimental study. Setting and Duration: Animal house,Bhitai Dental and Medical College Mirpurkhas and Agriculture University Tando Jam fromAnimal house from May 2015 - August 2016. Subjects and Methods: Sixty rats were equallydivided into 3 groups Group 1- Controls (0.9% isotonic saline), Group 2- (CCl4 CCl4 1.0mg/kg intraperitoneal) and Group 3- (CCl4+ GkbE). Blood samples were collected at end ofexperiment from tail veins. Liver was obtained after rat sacrifice by cervical dislocation. Tissuewas fixed in formaldehyde and embedded in paraffin. Microscopy of 3μ tissue sections wasperformed after H & E staining. Statistix 10.0 (USA) software was used for data analysisat 95% confidence interval. Results: Four weeks GkbE administration in CCl4 rat showedsignificant amelioration of liver enzymes and improved liver histology (p=0.0001). In GkbEtreated rats, the histological changes of degeneration, fatty change, inflammatory cellinfiltration, sinusoid congestion and necrosis was minimal (p=0.0001). GkbE was provedof mitigating the hepatocellular injury inflicted by carbon tetrachloride. Conclusion: GkbEmitigates the carbon tetrachloride induced liver injury in rat model. GkbE may be used in drugand chemical induced liver injury.
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