RESUMO -As espécies Ipomoea grandifolia, I. nil e Merremia aegyptia tornaram-se importantes infestantes em diferentes sistemas de cultivo, causando problemas principalmente na colheita. O objetivo deste trabalho foi determinar o comportamento germinativo dessas espécies em diferentes condições de temperatura (15, 20, 25, 30 e 35 o C), luz (presença e ausência) e profundidade de semeadura (0; 0,5; 1; 5; 10; 12; 15; e 20 cm) em solos com diferentes texturas. Os resultados evidenciaram que as espécies apresentaram maior germinação na faixa de temperatura entre 20 e 25 ºC. Houve maior capacidade de germinação quando elas foram submetidas à ausência de luz. Entre as espécies estudadas, I. grandifolia apresentou maior capacidade de germinar em maiores profundidades no solo arenoso. No solo argiloso, as espécies de Ipomoea spp. avaliadas apresentaram maior germinação na superfície. M. aegyptia germinou melhor na superfície do solo arenoso comparado ao argiloso, porém apresentou melhor capacidade de germinação em maiores profundidades no solo argiloso em relação às demais espécies avaliadas. Palavras-chave: biologia, plantas daninhas, corda-de-viola.ABSTRACT -Ipomoea grandifolia, I. nil, and Merremia aegyptia have been important weeds to different crops, causing problems mainly in culture systems. The objective of this work was to evaluate the germinative behaviour of these species under different conditions of temperature (15, 20, 25, 30, and 35 o C), light (presence and absence) and sowing depth (0; 0,5; 1; 5; 10; 12; 15; and 20 cm) in soils with different textures. The results showed that species incubated at 20 up to 25 ºC presented optimal germination. The species presented better germination capacity under no light condition. In relation to soil texture, the results showed that I. grandifolia presented better capacity of germination at greater depths in sandy-clay soil. Both Ipomoea species presented better germination on heavy-clay soil surface. M. aegyptia presented better germination on sandy-clay surface compared to heavy-clay soil. However, germination at greater depths was better in heavy-clay soil.

Sourgrass(Digitaria insularis, L. Mez ex Ekman)is a weed that requires high rates of glyphosate ((N-[phosphonomethyl]-glycine) forcontrol, verylittle of the herbicide applied isabsorbed by theleaves. Morphological and histological differences in leaves of glyphosate-resistant and glyphosate-susceptible plantsshould explain the contrast of variance between herbicide susceptibility. Leaves of different growth andphenological stages were collected and submitted to histological andelectron microscopy scanning analysis. Those plants were also submitted to a glyphosate dose-response curve analysis. The results suggest thatbiotypes exhibits differences infoliar structures that can influence the uptake and translocation of glyphosate. A thinparenchyma, lesser distance amongvascular bundles, and higher phloem sizewere found in resistant biotypes (differences close to 10%). Minor stomata number and higher epicuticular waxy deposition in stomata and leaf surface were found in resistant plants when leaves came from regrowth, leading to a possiblelesser herbicide absorption. Resistant plants showed absence of trichomes in late stages of development (64% less trichomes than susceptible plants). The higher concentration of trichomes in susceptible plants can be an absorption pathfor glyphosate getthrough the cuticle.Keywords: Absorption, herbicide resistance, parenchyma,trichomes A anatomia foliar de capim-amargoso influencia na resistência ao glyphosate? ResumoO capim-amargoso (Digitaria insularis, L. Mez ex Ekman) é uma plantadaninha que requer altas dosesde glifosato ((N-[fosfonometilo] glicina) para seu controle, sendo pouco do herbicida aplicado absorvido pelas folhas. Diferenças morfológicas e histológicas em folhas de biótipos resistentes e suscetíveis ao glifosato podem explicar a susceptibilidade diferencial ao herbicida. Folhas de diferentes estádios de crescimento de plantas foram coletadas e submetidas à análise histológica e de microscopia eletrônica de varredura. Essas plantas foram também submetidas a uma curva de dose-resposta ao glifosato. Os resultados sugerem diferenças foliares que podem influenciar a absorção e translocação de glyphosate. Um parênquima menos espesso, menores distâncias entre feixes vasculares, e maior tamanho de floema no biótipo resistente em relação ao biótipo suscetível foram observados (diferenças próximo a 10%). Menor número de estômatos e maior deposição de cera epicuticular em estômatos e superfície foliar nos biótipos resistentes foram encontradas quando a folha veio do rebrote, levando a uma possível menor absorção do herbicida. Em biótipos resistentes encontrou-se aausência de tricomas nos últimos estágios do desenvolvimento (64% a menos do que os tricomas plantas suscetíveis). A maior concentração de tricomas em plantas sensíveis, podem ser uma via de absorção para o glyphosate por entre a cutícula. Palavras-chave:Resistência a herbicidas, absorção, tricomas, parênquima

e FILHO R.V.2 RESUMO -O glyphosate é um herbicida não seletivo que inibe a produção de aminoácidos aromáticos essenciais à sobrevivência de plantas. Esse herbicida é disponibilizado no mercado em distintas formulações que podem apresentar diferenças no controle de espécies. A mistura de herbicidas é uma ferramenta importante no controle de plantas daninhas resistentes, sendo uma opção à mistura de inibidores da ACCase ao herbicida glyphosate. Este trabalho contou com a aplicação de diferentes misturas de quatro graminicidas e três formulações de glyphosate em dois estádios vegetativos de plantas de capim-amargoso resistente a esse herbicida. O controle químico foi avaliado por notas visuais de controle, aos 7, 14 e 28 dias após a aplicação, e pela medida de biomassa fresca das plantas, aos 28 dias depois da aplicação. As misturas foram avaliadas como antagonísticas, sinergísticas ou aditivas, segundo o modelo proposto por Colby. Dos tratamentos utilizados, conclui-se que todos são efetivos no manejo da resistência de Digitaria insularis, com exceção do uso de glyphosate isolado, porém o controle deve ser feito em estádios iniciais de desenvolvimento da planta. Em estádios mais desenvolvidos, o melhor controle se deu na associação de quizalofop às formulações sal de amônio e sal potássico de glyphosate; contudo, outras medidas de controle, além da associação de produtos, devem ser tomadas. Palavras-chave: graminicidas, Digitaria insularis, associação de herbicidas. ABSTRACT -Glyphosate is a non-selective herbicide that inhibits the production of essential amino acids for plant survival. This herbicide is available in the market in different formulations

The physicochemical properties of soil are fundamental to quantification of the fate of herbicides. Thus, the aim of this research was to evaluate the fate of diuron, hexazinone, and metribuzin in five soils (Clay-1, Clay-2, Loam-1, Loam-2, and Sand), presenting variation in clay content, cation exchange capacity (CEC), pH, and organic carbon (OC). Herbicides radiolabeled with C were applied, and theC-CO released from mineralization was trapped in 0.2 mol L sodium hydroxide solution. The degradation ratio, as well as herbicide-bound residues (non-extractable), transformation products, and residues extractable from soil, was also evaluated. Average C-CO evolution accumulated for diuron mineralization was higher (22.24%) than hexazinone (7.73%) and metribuzin (3.20%). The degradation time half-life (DT50) values for hexazinone correlated with soil OC content. Although no correlation between soil properties and DT50 values was found for metribuzin, the degradation rate and total degree of mineralization were low in sand soil for metribuzin. Regarding diuron, OC content and CEC value appear to be related to mineralization and degradation rate, respectively. Differences in soil properties can influence the persistence and fate of herbicides, affecting their impact on the environment, weed control, and possible effects on subsequent crops.

The ethanol production in Brazil is carried out by fed-batch or continuous process with cell recycle, in such way that bacterial contaminants are also recycled and may be troublesome due to the substrate competition. Addition of sulphuric acid when inoculum cells are washed can control the bacterial growth or alternatively biocides are used. This work aimed to verify the effect of chlorine dioxide, a well-known biocide for bacterial decontamination of water and equipments, against contaminant bacteria (Bacillus subtilis, Lactobacillus plantarum, Lactobacillus fermentum and Leuconostoc mesenteroides) from alcoholic fermentation, through the method of minimum inhibitory concentration (MIC), as well as its effect on the industrial yeast inoculum. Lower MIC was found for B. subtilis (10 ppm) and Leuconostoc mesenteroides (50 ppm) than for Lactobacillus fermentum (75 ppm) and Lactobacillus plantarum (125 ppm). Additionally, these concentrations of chlorine dioxide had similar effects on bacteria as 3 ppm of Kamoran® (recommended dosage for fermentation tanks), exception for B. subtilis, which could not be controlled at this Kamoran® dosage. The growth of industrial yeasts was affected when the concentration of chlorine dioxide was higher than 50 ppm, but the effect was slightly dependent on the type of yeast strain. Smooth yeast colonies (dispersed cells) seemed to be more sensitive than wrinkled yeast colonies (clustered cells/pseudohyphal growth), both isolated from an alcoholproducing unit during the 2006/2007 sugar cane harvest. The main advantage in the usage of chlorine dioxide that it can replace antibiotics, avoiding the selection of resistant populations of microorganisms.

Glyphosate is now the most widely used herbicide; after years of frequent utilization, resistant weeds were selected, mainly due to widespread adoption of glyphosate-resistant crops and no-tillage sowing system. Increasing difficulty in controllingChloris polydactylawith glyphosate has been noticed in agricultural areas. Here, the susceptibility level of variousC. polydactylaaccessions from Brazil is examined. Two whole-plant studies were conducted to confirm the presence and extent of glyphosate susceptibility among accessions, which involved the application of seven glyphosate doses on four accessions. The four accessions showed different glyphosate susceptibilities. The “Matão” accession presented major tolerance for glyphosate compared to “Palotina” accessions. “Jaboticabal” plants showed an intermediary susceptibility. The resistance factor (RF) was 3.76 between the “Matão” and “Palotina” accessions. All biotypes died at 2,880 g ae ha−1glyphosate.

Glyphosate has been associated with control failures for Spermacoce verticillata in some Brazilian States. The objective of this work was to evaluate and determine the possible causes of this and propose alternative herbicides to use. Glyphosate was applied at three plant stages of development (2-4 leaves, 4-6 leaves and full bloom) where foliar absorption and translocation of glyphosate to various plants parts and time were analysed using the 14 C technique. Data were submitted to nonlinear regressions and analysis of variance, where means were compared by a Tukey test at 5% probability. Plant control by the application of different herbicides (19) in the same three stages of development of weed was evaluated. Twenty-one days after herbicide application, control was visually evaluated and data analysed and means were compared.Due to absorption and/or translocation problems, S. verticillata plants were not controlled by glyphosate. Plants with 4-6 leaves showed lower absorption and translocation of the herbicide to the leaf/root regions compared with plants at the beginning of their development. Plants at full bloom showed lower translocation of the herbicide to the root. In addition to the application of glyphosate at early stages of development, the application of paraquat, flumioxazin and mixtures of glyphosate with flumioxazin or cloransulam is recommended. Late applications could result in poor control, principally if glyphosate is used. Therefore, early applications are strongly recommended for control of this species.

To gain a better understanding of the physiology of the herbicide aminocyclopyrachlor in young plants of trumpet flower, the uptake and translocation were evaluated after the application of herbicide. This was determined by treating individual leaves with formulated herbicides plus (14)C-aminocyclopyrachlor after the application of the formulated herbicide. This experiment used a randomized experimental design with three replications. In addition, field studies were conducted to assess the effectiveness of foliar applications of aminocyclopyrachlor in association with metsulfuton-methyl. The plant absorbed 20% of the herbicide applied. The translocation percentage did not surpass 5% of the total amount applied. Only 1% of the herbicide applied was translocated to the roots. Rate of 40 + 13 g a.i. 100 L(-1) of aminocyclopyrachlor+metsulfuron-methyl was effective to control T. stans.