The present study was undertaken to assess the potential uses of the essential oil obtained from Ocotea nutans (Nees) Mez. The hydrodistilled essential oil from O. nutans leaves was analyzed by gas chromatography-mass spectrometry. Fifty-eight compounds representing 87,29% of the total leaf essential oil components were identified, of which biciclogermacrene(11.41%), germacrene-D (4.89%), bisabolol-11-ol(3.73%) and spathulenol (3.71%) were the major compounds. The essential oil from O. nutans were tested for antibacterial activity using the minimum inhibitory concentracion (MIC) method, Artemia salina method, larvicidal activity in Aedes aegypti, and antioxidant capacity.The antioxidant activity measured by the phosphomolybdenum complex and Prussian blue method had positive results. The minimum inhibitory concentration for the microorganisms tested allowed moderate inhibitionfor Enterococcus faecalis (MIC=500 µg/mL). Artemia salina were toxic to the organisms in the study (LC 50 = 71,70 μg /mL). The essential oil showed remarkable larvicidal activity potencial (LC 50 = 250 µg/mL). The present results showed that O. nutans essential oil has potential biological uses.
The essential oil composition of the Ocotea bicolor, native plant of Brazil, was studied for the first time. The essential oil of the leaf was obtained by hydrodistillation and analyzed by GC/MS. The analytical procedure revealed a predominance of sesquiterpenes, δ-cadinene (7.39%), β-sesquiphellandrene (6.67%), β-elemene (5.41%) and α-cadinol (5,23%). The essential oil was submitted to brine shrimp toxicity evaluation, antioxidant and antibacterial tests. The antioxidant activity by the formation of phosphomolybdenum complex method presented positive results. The minimum inhibitory concentration (MIC) values were higher than 1000 μg/mL for the microorganisms tested. Toxicity activity revealed LC50 results of 40.10 (μg/mL), being toxic to the organisms in this study.
Based on medicinal use of Dalbergia species, the present study aimed to evaluate D. brasiliensis leaves and barks crude extracts and fractions' antioxidant and phytotoxic potential, employing allelopathic essays and 3 antioxidant essays: Phosphomolybdenum complex reduction, DPPH free radical scavenging and TBARS (lipid peroxidation). Concerning Phosphomolybdenum complex reduction potential, leaves chloroform fraction (LCF) and barks ethyl acetate fraction (BEAF) overcame rutin's reduction potential in 90% and 23%. DPPH essay revealed leaves ethyl acetate fraction (LEAF) antioxidant potential (IC 50 de 40,629 µg/mL), given standards ascorbic acid (IC 50 = 4,503 µg/mL) and rutin (IC 50 = 7,330 µg/mL) activities. TBARS essay demonstrated that leaves hexane fraction (LHF) (51%) and barks ethyl acetate fraction (BEAF) (41%) stand out when compared to BHT antioxidant activity (54%). Regarding phytotoxicity, the lowest crude extracts and fractions concentration (250 µg/mL) employed were able to inhibit L. sativa and A. cepa primary root initial growth, specially leaves hexane fraction (LHF) and leaves chloroform fraction (LCF). Dalbergia brasiliensis fractions might function as stress inhibitors in oxidant systems and alter meristem cellular division, due to present substances' activity.
This study aimed to investigate the anatomy and histochemistry of Mollinedia clavigera leaves and stems through photonic microscopy and scanning electron microscopy. Noteworthy features of leaves were: presence of paracytic stomata on both surfaces; simple as well as bifurcate non-glandular trichomes; prismatic calcium oxalate crystals; flat-convex midrib with a central and two dorsal bundles; concave-convex HIGHLIGHTS This is the first report of the microscopic study of Mollinedia clavigera Light microscopy, SEM, EDS and histochemistry analysis were made to support anatomical and chemical characteristics Non-glandular unicellular, long uniseriate, and short bifurcate trichomes were observed • Prismatic crystals of calcium oxalate were observed on the surfaces of leaves Homem, I. C. M.; et al
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