Dalbergia brasiliensis Vogel Extracts in vitro Antioxidant Activity and Phytotoxic Effect in Lactuca sativa and Allium cepa

Abstract

Based on medicinal use of Dalbergia species, the present study aimed to evaluate D. brasiliensis leaves and barks crude extracts and fractions' antioxidant and phytotoxic potential, employing allelopathic essays and 3 antioxidant essays: Phosphomolybdenum complex reduction, DPPH free radical scavenging and TBARS (lipid peroxidation). Concerning Phosphomolybdenum complex reduction potential, leaves chloroform fraction (LCF) and barks ethyl acetate fraction (BEAF) overcame rutin's reduction potential in 90% and 23%. DPPH essay revealed leaves ethyl acetate fraction (LEAF) antioxidant potential (IC 50 de 40,629 µg/mL), given standards ascorbic acid (IC 50 = 4,503 µg/mL) and rutin (IC 50 = 7,330 µg/mL) activities. TBARS essay demonstrated that leaves hexane fraction (LHF) (51%) and barks ethyl acetate fraction (BEAF) (41%) stand out when compared to BHT antioxidant activity (54%). Regarding phytotoxicity, the lowest crude extracts and fractions concentration (250 µg/mL) employed were able to inhibit L. sativa and A. cepa primary root initial growth, specially leaves hexane fraction (LHF) and leaves chloroform fraction (LCF). Dalbergia brasiliensis fractions might function as stress inhibitors in oxidant systems and alter meristem cellular division, due to present substances' activity.

Antiplasmodial, antioxidant and toxicological study of leaves extracts of Dalbergia katangensis Lecheneaud (Fabaceae) from Eastern DR Congo

“…This study (figure 1) confirms the interesting antioxidant activity of aqueous and methanolic extracts of Dalbergia katangensis leaves previously reported [20]. At the genus level, The antioxidant activity at DPPH, of the leaves of some species of the genus Dalbergia has been previously reported, in particular for D. paniculata Roxb, IC50 = 70.6 µg/mL [42], D. odorifera T.C.Chen, IC50 = 5.1 ± 0.12 µg/mL [43], D. brasiliensis Vogel, IC50 = 74.5 ± 1.3 µg/mL [44] and D. sissoo, IC50 = 3.2 µg/mL [45]. Obviously, D. katangensis (ME IC50 = 0.58 μg/mL) exhibits an antioxidant activity greater than that of the above-mentioned plant species.…”

Toxicidade preliminar dos extratos e frações obtidas das folhas e das cascas do caule da Dalbergia brasiliensis

“…A espécie D. brasiliensis é uma arvore caducifólia, com 4 a 20 m de altura (CARVALHO, 2004) o qual ocorre de forma natural no Brasil (Cerrado e Floresta Atlântica), com poucos estudos na literatura. A atividade antioxidante desta espécie foi relatada por Dalarmi et al (2017), o qual constataram que todos os extratos provenientes das cascas e das folhas, apresentaram atividade, principalmente as frações clorofórmio e acetado de etila da casca e as frações hexano e clorofórmio da folha.…”