Ocotea nutans (Nees) Mez (Lauraceae): chemical composition, antioxidant capacity and biological properties of essential oil

Abstract

The present study was undertaken to assess the potential uses of the essential oil obtained from Ocotea nutans (Nees) Mez. The hydrodistilled essential oil from O. nutans leaves was analyzed by gas chromatography-mass spectrometry. Fifty-eight compounds representing 87,29% of the total leaf essential oil components were identified, of which biciclogermacrene(11.41%), germacrene-D (4.89%), bisabolol-11-ol(3.73%) and spathulenol (3.71%) were the major compounds. The essential oil from O. nutans were tested for antibacterial activity using the minimum inhibitory concentracion (MIC) method, Artemia salina method, larvicidal activity in Aedes aegypti, and antioxidant capacity.The antioxidant activity measured by the phosphomolybdenum complex and Prussian blue method had positive results. The minimum inhibitory concentration for the microorganisms tested allowed moderate inhibitionfor Enterococcus faecalis (MIC=500 µg/mL). Artemia salina were toxic to the organisms in the study (LC 50 = 71,70 μg /mL). The essential oil showed remarkable larvicidal activity potencial (LC 50 = 250 µg/mL). The present results showed that O. nutans essential oil has potential biological uses.

GC-MS Profile and Enhancement of Antibiotic Activity by the Essential Oil of Ocotea odorífera and Safrole: Inhibition of Staphylococcus aureus Efflux Pumps

“…According to Nazzaro et al [34], Gram-negative bacteria are more resistant to the penetration of essential oils due to the presence of an outer layer of lipopolysaccharides. Accordingly, Betim et al [35], comparing the antibacterial activities of O. odorífera and O. nutans, demonstrated that the essential oil of O. odorífera presented more potent effects. In addition, they found that both essential oils were more effective against Gram-positive bacteria, corroborating the findings of the present research.…”

Atividade larvicida de extratos de Diplodia pinea frente à Aedes aegypti

“…A metodologia empregada para avaliação quantitativa da atividade larvicida foi adaptada de Garcez et al, (2013) e Betim et al, (2019). Para a avaliação da atividade dos extratos, ovos de A. aegypti (cepa Rockefeller cedida pela Fundação Oswaldo Cruz) foram eclodidos em laboratório a 25 ± 3 °C, umidade relativa de 80%, em estufa BOD, e alimentados com ração para peixes (Alcon basic, complexo MEP 200) até o terceiro estágio de desenvolvimento larval.…”

Preliminary evaluation of the larvicidal activity of extracts and fractions from Ocotea nutans (Nees) Mez against Aedes aegypti

“…This native Brazilian species inhabits mixed ombrophilus forests and is found in the states of Paraná, Sao Paulo, Espírito Santo, Minas Gerais, and Bahia 6 . The essential oil of O. nutans contains bicyclogermacrene (11.41%), germacrene-D (4.89%), bisabolol-11-ol (3.73%), and spathulenol (3.71%) as the major compounds; it shows toxicity against A. aegypti and controls third-stage larvae 7 , with an LC 50 of 250 µg.mL -1 .…”

“…The following methodology was adapted from Betim et al 7 and Garcez et al 9 . The eggs of A. aegypti (Rockefeller strain provided by the Oswaldo Cruz Foundation) were reared under laboratory conditions (27±3 °C, relative humidity of 80%, and incubated in a Bio-Oxygen Demand incubator) by feeding with fish feed (Aldon basic, MEP 200 complex) from hatching until the third stage of larval development.…”

“…Narciso et al 10 examined the larvicidal properties of lignin extracted from the hexane fractions of stems from O. cymbarum Kunth and reported high mortality against A. aegypti larvae. Betim 7 et al examined essential oils (terpenoid-based constituents) from O. nutans , and this mixture could control third-stage larvae, with an LC 50 of 250 µg.mL -1 . Terpenes and derivatives have larvicidal properties, and it is suggested that their liposoluble characteristics have a strong influence on the mortality of larvae 11 .…”