Objective: Myrmecodia pendens is often used as a traditional medicine to treat various diseases. A previous study showed that ethanol extract of sarang semut plant (hypnophytum furmicarum jack) 4.65 mg was effective in fastening establishment of socket granulation tissue after tooth extraction; likewise, the myrmecodia pendens at 3% also fastens the healing process of soft-tissue wound after tooth extraction. The aim of this study is to assess the effect of ethanol extract of 10% myrmecodia pendens on the expression of TGF-β1 and determine the number of osteoblasts that remain after tooth extraction. Material and Methods:This study used 24 male marmots divided into 2 groups. All of the groups were extracted in the 1st left-incisor mandibula and 0.1 ml of 0.5% CMC was applied into the socket control group. The treatment group was given 0.1 ml ethanol extract of myrmecodia pendens 10% dissolved with CMC 0.5%. On day 3, 7, 14 and 21, marmots were decapitated and histopathologic preparations were observed to study the expression of TGF-β1 and the number of osteoblast cells. Data were analyzed using Kruskall-Wallis test with a probability p < 0.05. Results: Based on the Kruskal-Wallis test, there was a significant difference in the TGF-β1 expression and the number of osteoblast cells after the administration of ethanol extract of myrmecodia pendens between the control group and treatment group treatment (p < 0.05). Conclusion:The ethanol extract of myrmecodia pendans can fasten the healing of the wound resulting from tooth extraction; it accelerates the healing process by increasing the expression of TGF-β1 and the number of osteoblast cells.Keywords: Myrmecodia pendens, Osteoblasts, TGF-β1, Tooth extraction Cite this Article: Ismardianita E, Nasrul E, Yanwirasti, Hemiawati M. 2017. Effect of ethanol extract of myrmecodia pendens on TGF-β1 expression and osteoblast cells after tooth extraction (experimental research on cavia cobaya). Journal of Dentomaxillofacial Science 2(3): 150-154.
Tooth extraction is a common thing, more often after extraction patients have any complication, to prevent it, physician provide some drugs from chemicals that often give adverse effects, so that required for a safe substitute medicine, which derived from herbs. One of the herbs are often used by people in the Mentawai Islands is a sarang semut's root species of Hypnophytum formicarum jack. This study aimed to determine the effect of sarang semut's extract orally for granulation tissue formation in wound healing after tooth extraction. This research is experimental with posttest only control group design. Subjects were 48-animal guinea pigs, were divided into 4 groups: group I (control 0.5% CMC, treatment group II, III, IV with 4,65mg, and 9.3 mg 6,2 mg dose tuber ethanol extract dissolved anthill in CMC 0.5%), were given 3 ml orally, 3 times a day until the day of decapitation. Early research conducted identification and phytochemical screening, this plant belongs to the species Hypnophytum formicarum jack, contain flavonoids, phenols, triterpenoids and tannins. The data was analyzed with non-parametric Kruskal-Wallis, to see the significance between doses used Mann-Whitney. The results showed that the extract of sarang semut effect (p < 0.05) on the formation of granulation system in wound healing after tooth extraction. The most effective concentration was 4.65 mg
Streptococcus sp adalah salah satu bakteri yang banyak ditemukan pasca pencabutan gigi, untuk itu pencegahan infeksi dapat dilakukan dengan memberikan terapi antibiotik. Cabe rawit (Capsicum Frustecens, L) adalah salah satu tanam herbal yang memiliki efek sebagai anti mikroba terhadap bakteri Streptococcus Sp. Tujuan penelitian ini adalah untuk mengetahui pengaruh pemberian ekstrak cabai rawit (Capsicum Frustecens, L) terhadap bakteri Streptococcus Sp pada soket pasca pencabutan gigi. Jenis penelitian adalah eksperimental laboratorium dengan rancangan Post test only control group design, sampel penelitian yang digunakan adalah koloni bakteri Streptococcus sp yang dikultur langsung dari soket pasien pasca pencabutan gigi. Pengenceran ekstrak cabai rawit (Capsicum Frustecens, L) yaitu menggunakan 4 konsentrasi (20%, 40%, 60% dan 80%). Data hasil penelitian di uji dengan one way anova. Hasil penelitian menunjukan nilai rata-rata konsentrasi 20% adalah (11,01 mm), konsentrasi 40% (18,45 mm), konsentrasi 60% (18,62 mm) dan pada konsentrasi 80% yaitu19,34 mm, uji Anova didapat nilai fhitung> ftabel yaitu 6,211>3.24 dan p=0,005<0,05, dapat disimpulkan ekstrak cabai rawit (Capsicum Frustecens, L) dapat menghambat pertumbuhan bakteri Streptococcus sp pada konsentrasi 20%, 40%, 60% dan 80%, uji LSD ekstrak cabai rawit (Capsicum Frustecens, L). Hasil penelitian pengaruh pemberian ekstrak cabe rawit (Capsicum Frustecens, L) terhadap bakteri Streptococcus Sp pada soket pasca pencabutan gigi dengan dosis yang efektif 40%.
Introduction: Tooth extraction causes tissue damage around the socket. Wound healing is essential for repairing damaged tissues. One of the medicinal plants often used is ant-plant (Hydnophytum formicarum), which contains flavonoids, triterpenoids, saponins and tannins. This research was aimed to determine the effect of ant-plant ethanol extract on the density of collagen fibers for wound healing after tooth extraction in the guinea pig (Cavia cobaya). Methods: The type of research was true experimental with a post-test only control group design. The study used 48 male guinea pig divided into 4 groups. Experimental groups received lower left incisor extraction, the control group was orally administered with 0.5% CMC, and the treatment group was administered with ant-plant extract dissolved with 0.5% CMC with dose of 4.65 mg, 6.2 mg, and 9.3 mg respectively; each guinea pig was given 3 cc of the extract 3 times a day until the termination day. On day 3, 7, 14, and 21 respectively, the guinea pigs were terminated and to make the histological preparations. The difference in collagen fibers density of the two observation groups was tested with one-way ANOVA, and the LSD test to analyse the significance between groups. Results: Application of ant-nest ethanol extract showed significant effects on the density of collagen fibers in the wound healing process after tooth extraction. Results of the one-way ANOVA test showed significant value on all treatment groups (p < 0.05). There were also found significant differences between the control group and the treatment group (p < 0.05). Conclusion: Ant-plant (Hydnophytum formicarum) ethanol extract increase the collagen fibers density on the wound healing after tooth extraction with the effective dose of 4.65 mg.Keywords: Ant-plant, Hydnophytum formicarum, wound healing, collagen, guinea pig, Cavia cobaya.
Introduction: Tooth extraction causing injuries, to accelerate wound healing was given medication. Drugs commonly used for wound healing after tooth extraction is povidone iodine act as bacteriostatic for all germs. Excessive use of povidone iodine can cause side effects such as itching, pain in the area around the wound, swelling and dermatitis, so it sought a safe substitute drugs derived from herbs. One of the herbs are often used by people in the Mentawai Islands is a Sarang Semut's root species of Hypnophytum formicarum. This study was aimed to determine the effect of sarang semut’s extract orally for angiogenesis in wound healing after tooth extraction. Methods: This research was experimental with posttest only control group design. Subjects were 48-animal marmots were divided into 4 groups, will be deprived of his left mandibular insisivus. Group I (control 0,5% CMC, treatment group II, III, IV with 4,65mg, and 9.3 mg 6,2 mg dose tuber ethanol extract dissolved anthill in CMC 0.5%), were given 3 ml orally, 3 times a day until the day of decapitation. Futher histological preprations made by HE staining and the observed area is apex, medial, servical,and lateral used 400x magnification. The data was analyzed with parametric test One-Way ANOVA, and to see the significance between dose used LSD test. Results: The results showed that the extract of sarang semut effect (p<0,05) on the angiogenesis in wound healing after tooth extraction. Conclusion: The most effective concentration was 4,65 mg.
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