The effect of ant-plant (Hydnophytum formicarum) ethanol extract on collagen fibers for wound healing after tooth extraction in the guinea pig (Cavia cobaya)

Abstract

Introduction: Tooth extraction causes tissue damage around the socket. Wound healing is essential for repairing damaged tissues. One of the medicinal plants often used is ant-plant (Hydnophytum formicarum), which contains flavonoids, triterpenoids, saponins and tannins. This research was aimed to determine the effect of ant-plant ethanol extract on the density of collagen fibers for wound healing after tooth extraction in the guinea pig (Cavia cobaya). Methods: The type of research was true experimental with a post-test only control group design. The study used 48 male guinea pig divided into 4 groups. Experimental groups received lower left incisor extraction, the control group was orally administered with 0.5% CMC, and the treatment group was administered with ant-plant extract dissolved with 0.5% CMC with dose of 4.65 mg, 6.2 mg, and 9.3 mg respectively; each guinea pig was given 3 cc of the extract 3 times a day until the termination day. On day 3, 7, 14, and 21 respectively, the guinea pigs were terminated and to make the histological preparations. The difference in collagen fibers density of the two observation groups was tested with one-way ANOVA, and the LSD test to analyse the significance between groups. Results: Application of ant-nest ethanol extract showed significant effects on the density of collagen fibers in the wound healing process after tooth extraction. Results of the one-way ANOVA test showed significant value on all treatment groups (p < 0.05). There were also found significant differences between the control group and the treatment group (p < 0.05). Conclusion: Ant-plant (Hydnophytum formicarum) ethanol extract increase the collagen fibers density on the wound healing after tooth extraction with the effective dose of 4.65 mg.Keywords: Ant-plant, Hydnophytum formicarum, wound healing, collagen, guinea pig, Cavia cobaya.

Effects of the Hydnophytum formicarum plant extract on collagen density, angiogenesis, wound length, and re-epithelialization in wound healing: Experimental study on rats

“…9,10 A study conducted by Velanita et al found that the ad ministration of the Hydnophytum formicarum plant ex tract orally at a dose of 4.65 mg was able to significantly increase the production of collagen fibers in wound heal ing in the oral cavity after tooth extraction. 11 This is in contrast to the results of the present study, which indi cated that collagen density in the control and treatment groups did not present significantly different values, although there was a statistically significant interaction between the use of the extract and the necropsy day, af fecting collagen density. This interaction indicates that the Hydnophytum formicarum plant extract may change the value of collagen density when used for 14 days.…”

“…Moreover, the extract was applied topically, whereas in previous studies, it was administered orally. 9,11 With regard to angiogenesis, this study indicates that the topically applied Hydnophytum formicarum plant extract can significantly reduce angiogenesis that occurs during the early, middle and late prolifera tion phases. This result is consistent with research by Ismardianita et al, who concluded that the oral adminis tration of the Hydnophytum formicarum plant extract at a dose of 6.2 mg was able to significantly reduce angio genesis in the proliferation phase.…”