The Effect of Ethanol Extract Of Sarang Semut Plant (Hypnophytum Formicarum) to Granulation Tissue for Wound Healing After Teeth Extraction Experimental Research on Marmot (Cavia cobaya)

Abstract

Tooth extraction is a common thing, more often after extraction patients have any complication, to prevent it, physician provide some drugs from chemicals that often give adverse effects, so that required for a safe substitute medicine, which derived from herbs. One of the herbs are often used by people in the Mentawai Islands is a sarang semut's root species of Hypnophytum formicarum jack. This study aimed to determine the effect of sarang semut's extract orally for granulation tissue formation in wound healing after tooth extraction. This research is experimental with posttest only control group design. Subjects were 48-animal guinea pigs, were divided into 4 groups: group I (control 0.5% CMC, treatment group II, III, IV with 4,65mg, and 9.3 mg 6,2 mg dose tuber ethanol extract dissolved anthill in CMC 0.5%), were given 3 ml orally, 3 times a day until the day of decapitation. Early research conducted identification and phytochemical screening, this plant belongs to the species Hypnophytum formicarum jack, contain flavonoids, phenols, triterpenoids and tannins. The data was analyzed with non-parametric Kruskal-Wallis, to see the significance between doses used Mann-Whitney. The results showed that the extract of sarang semut effect (p < 0.05) on the formation of granulation system in wound healing after tooth extraction. The most effective concentration was 4.65 mg

Effects of the Hydnophytum formicarum plant extract on collagen density, angiogenesis, wound length, and re-epithelialization in wound healing: Experimental study on rats

“…Moreover, the extract was applied topically, whereas in previous studies, it was administered orally. 9,11 With regard to angiogenesis, this study indicates that the topically applied Hydnophytum formicarum plant extract can significantly reduce angiogenesis that occurs during the early, middle and late prolifera tion phases. This result is consistent with research by Ismardianita et al, who concluded that the oral adminis tration of the Hydnophytum formicarum plant extract at a dose of 6.2 mg was able to significantly reduce angio genesis in the proliferation phase.…”

“…This result is consistent with research by Ismardianita et al, who concluded that the oral adminis tration of the Hydnophytum formicarum plant extract at a dose of 6.2 mg was able to significantly reduce angio genesis in the proliferation phase. 9 By contrast, research conducted by Putri and Ismardianita found that the orally administered Hydnophytum formicarum plant extract at a dose of 4.65 mg was able to significantly increase angio genesis. 13 Wound healing with minimal to no scarring oc curs in the oral mucosa and the fetal skin in the uterus, pre senting less angiogenesis as compared to wound healing in the skin; however, the capillaries become mature more rapidly.…”

“…A study conducted by Ismardianita et al found that the oral administration of the Hydnophytum formicarum plant extract had an inversely proportional relationship with the number of macrophages in the inflammatory phase of wound healing after tooth extraction. 9 Macro phages play an important role in the secretion of cytokines to attract fibroblasts that become an essential component in the proliferation phase. 10 As already known, fibroblasts are responsible for important actions that support wound healing, such as the production of collagen, angiogenesis, wound contraction, and reepithelialization.…”

“…4 Previous studies determined that decreasing the number of macrophages in the inflamma tory phase could be correlated with the suppressed fibro blast activities. 9,10 A study conducted by Velanita et al found that the ad ministration of the Hydnophytum formicarum plant ex tract orally at a dose of 4.65 mg was able to significantly increase the production of collagen fibers in wound heal ing in the oral cavity after tooth extraction. 11 This is in contrast to the results of the present study, which indi cated that collagen density in the control and treatment groups did not present significantly different values, although there was a statistically significant interaction between the use of the extract and the necropsy day, af fecting collagen density.…”